Program Integration and Management

摘要 本计划项目的计划集成和管理部分将确保沟通 以使每个项目和核心能够进行自己的研究并 接口与其他接口。此外，该构成部分将确保负责任地管理 计划项目的项目和核心。如上所述，带有实验室组件的3个项目将使用DNA 或者来自完全相同的肿瘤的RNA。行政核心一直负责制定 为了获得肿瘤而进行合作，并将保持统一的数据(见附录x)，与 关于诊断阶段、诊断年份和随访生存信息的关键信息(死亡日期 黑色素瘤，其他原因死亡，随访时活着)；Biospecimen核心将接收肿瘤， 评估它们的组织病理学特征(见Biospecimen核心)，如Breslow厚度、太阳 弹力增生症、肿瘤浸润性淋巴细胞(TIL)等，将H&E送至UNC进行Aperio ScanScope扫描以 与所有研究人员分享，从每个肿瘤和正常组织中提取DNA和RNA(在 我们没有来自血液或淋巴结的生殖系DNA的情况)，将DNA发送到项目1以进行靶向 突变分析(包括肿瘤和生殖系，包括CNV的评估)，将DNA发送到项目2进行 甲基化检测，并将RNA发送到项目3，使用纳米串分析microRNA和信使核糖核酸。这个 生物信息核心公司将开发用于存储、管理和共享个人生成的数据的工具 项目。它还将帮助每个项目进行分析，而不是将可用于 影响试验和甲基化试验，以及miRNA和mRNA分析。这些数据将是 可用于项目4，以及来自每个项目的原始数据，因此集成可以如上所述地进行。 在每两周一次的电话会议期间，将作为每个项目的PI监测每个项目的进展情况 (贝里克、托马斯、赫尔南多-蒙格和沈阳)和科尔(贝里克、托马斯、布萨姆和戈尔洛夫)在 指导委员会以及几个关键成员(Armos、Begg、Ernstoff和Orlow)。我们已经确立了 个人之间牢固的信任关系和困难的问题已经解决，因此 我们期待着这些良好的互动继续下去。

Abstract The Program Integration and Management component of this Program Project will assure communication among the Projects and Cores so that each Project and Core is able to conduct their own research and to interface among the others. Furthermore, this component will assure the responsible management of the Projects and Cores of the Program Project. As noted, the 3 projects with a laboratory component will use DNA or RNA from the exact same tumors. The Administrative Core has been responsible for developing the collaborations in order to obtain tumors and will maintain the harmonized data (see Appendix x), with the critical information as to Stage at diagnosis, year of diagnosis and follow up with survival information (Dead of Melanoma, Dead of Other Causes, and Alive at Follow up); the Biospecimen Core will receive the tumors, evaluate them for histopathologic characteristics (see Biospecimen Core), such as Breslow thickness, solar elastosis, tumor infiltrating lymphocytes (TILs), etc., send the H&E to UNC for Aperio ScanScope scanning to share with all investigators, extract DNA and RNA from each tumor, as well as uninvolved normal tissue (in cases where we don’t have germline DNA from blood or lymph nodes), send DNA to Project 1 for targeted mutation analysis (both tumor and germline, including evaluation of CNVs), send DNA to Project 2 for methylation assays, and send RNA to Project 3 for analysis of microRNA and mRNA using Nanostring. The Bioinformatics Core will develop tools for storage, management and sharing data generated by individual projects. It will also assist each project in analysis beyond the “pipeline” analyses that will be available for the IMPACT assay and the methylation assays, as well as the miRNA and mRNA analyses. These data will be available to Project 4 as will the raw data from each project, so that the Integration can occur as described. Progress in each project will be monitored during the bi-weekly Teleconferences as the PI’s of each project (Berwick, Thomas, Hernando-Monge and Shen) and core (Berwick, Thomas, Busam and Gorlov) are on the Steering Committee along with several key members (Armos, Begg, Ernstoff and Orlow). We have established a strong trusting relationship among the individuals and difficult issues have already been worked out and so we anticipate continuation of these excellent interactions.