Program Integration and Management

项目整合与管理

• 批准号: 10268361
• 负责人: MARIANNE BERWICK
• 金额: $ 1.2万
• 依托单位: UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-06-01 至 2023-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10268361
• 关键词: Adjuvant Therapy; American Joint Committee on Cancer; Bioinformatics; Biological Assay; Biological Markers; Biology; Blood; Breslow Thickness; Characteristics; Collaborations; Communication; Communication Tools; DNA; Data; Diagnosis; Evaluation; Goals; Individual; Laboratories; Messenger RNA; Methylation; MicroRNAs; Monitor; Mutation; Mutation Analysis; Normal tissue morphology; Patients; RNA; Research; Research Personnel; Resource Sharing; Scanning; Sex Differences; Solar elastosis; Stage at Diagnosis; TNM; Teleconferences; Trust; Tumor-Infiltrating Lymphocytes; Work; analysis pipeline; data harmonization; data sharing; follow-up; lymph nodes; melanoma; member; methylome; nano-string; outcome forecast; programs; tool; tumor

The Program Integration and Management component of this Program Project will assure communication among the Projects and Cores so that each Project and Core is able to conduct their own research and to interface among the others. Furthermore, this component will assure the responsible management of the Projects and Cores of the Program Project. As noted, the 3 projects with a laboratory component will use DNA or RNA from the exact same tumors. The Administrative Core has been responsible for developing the collaborations in order to obtain tumors and will maintain the harmonized data (see Appendix x), with the critical information as to Stage at diagnosis, year of diagnosis and follow up with survival information (Dead of Melanoma, Dead of Other Causes, and Alive at Follow up); the Biospecimen Core will receive the tumors, evaluate them for histopathologic characteristics (see Biospecimen Core), such as Breslow thickness, solar elastosis, tumor infiltrating lymphocytes (TILs), etc., send the H&E to UNC for Aperio ScanScope scanning to share with all investigators, extract DNA and RNA from each tumor, as well as uninvolved normal tissue (in cases where we don’t have germline DNA from blood or lymph nodes), send DNA to Project 1 for targeted mutation analysis (both tumor and germline, including evaluation of CNVs), send DNA to Project 2 for methylation assays, and send RNA to Project 3 for analysis of microRNA and mRNA using Nanostring. The Bioinformatics Core will develop tools for storage, management and sharing data generated by individual projects. It will also assist each project in analysis beyond the “pipeline” analyses that will be available for the IMPACT assay and the methylation assays, as well as the miRNA and mRNA analyses. These data will be available to Project 4 as will the raw data from each project, so that the Integration can occur as described. Progress in each project will be monitored during the bi-weekly Teleconferences as the PI’s of each project (Berwick, Thomas, Hernando-Monge and Shen) and core (Berwick, Thomas, Busam and Gorlov) are on the Steering Committee along with several key members (Armos, Begg, Ernstoff and Orlow). We have established a strong trusting relationship among the individuals and difficult issues have already been worked out and so we anticipate continuation of these excellent interactions.

本计划项目的计划整合和管理组件将确保沟通 在项目和核心之间，使每个项目和核心能够进行自己的研究， 与其他人的接口。此外，该构成部分还将确保对 项目和项目的核心。如上所述，3个具有实验室组成部分的项目将使用DNA 或RNA的基因。行政核心负责制定 合作，以获得肿瘤，并将保持协调的数据（见附录x），与 关于诊断时的分期、诊断年份和随访生存信息的关键信息（死亡 黑色素瘤，其他原因死亡，随访时存活）;生物样本核心将接收肿瘤， 评估它们的组织病理学特征（见生物标本核心），如Breslow厚度，日光 弹性组织变性、肿瘤浸润淋巴细胞（TIL）等，将H&E发送至ESPER进行Aperio ScanScope扫描， 与所有研究人员共享，从每个肿瘤以及未涉及的正常组织中提取DNA和RNA（在 我们没有来自血液或淋巴结的生殖系DNA的情况下），将DNA发送到项目1进行靶向 突变分析（肿瘤和生殖系，包括CNV的评估），将DNA发送到项目2， 甲基化分析，并将RNA发送到项目3，用于使用Nanostring分析microRNA和mRNA。的 生物信息学核心将开发用于存储，管理和共享个人生成的数据的工具 项目它还将协助每个项目进行“编审中”分析以外的分析， IMPACT测定和甲基化测定，以及miRNA和mRNA分析。这些数据将 项目4和每个项目的原始数据一样可用，因此可以按照所述进行集成。 在每两周一次的会议期间，将作为每个项目的PI对每个项目的进度进行监控 （贝里克、托马斯、埃尔南多-蒙赫和沈）和核心（贝里克、托马斯、布萨姆和戈尔洛夫）在 指导委员会沿着有几个主要成员（阿莫斯，贝格，恩斯特夫和奥洛）。我们建立 个人之间的牢固信任关系和困难问题已经解决， 我们期待着这种良好的互动继续下去。