A bacterial biosensor for intracellular metal availability in the gut

用于肠道内细胞内金属可用性的细菌生物传感器

基本信息

  • 批准号:
    10353307
  • 负责人:
  • 金额:
    $ 5.85万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2022
  • 资助国家:
    美国
  • 起止时间:
    2022-04-22 至 2022-12-16
  • 项目状态:
    已结题

项目摘要

PROJECT SUMMARY Of the foodborne bacterial, protozoal and viral diseases, non-typhoidal Salmonella enterica cause the largest burden of illness and death worldwide. The most common human clinical isolates are Salmonella enterica serovars Typhimurium (S. Typhimurium, STm) and Enteriditis (S. Enteriditis). Infection can cause either a self- limiting gastroenteritis or a life-threatening, invasive disease in immunocompromised individuals. During enteric infection, STm adopts both extracellular and intracellular lifestyles, colonizing the intestinal lumen as well epithelial cells and phagocytes in the intestinal mucosa. Here we will study how host-mediated restriction of transition metals, known as nutritional immunity, affects STm colonization of the gut. The NRAMP family of proteins are “promiscuous” transporters of divalent cations that play a major role in metal ion homeostasis from bacteria to man. In mammals, there are two NRAMP genes, SLC11A1 and SLC11A2 in humans and Slc11a1 and Slc11a2 in mice. Slc11a1 is restricted to the myeloid lineage whereas Slc11a2 is ubiquitously expressed. Whilst the role of Slc11a1 in controlling intracellular bacterial and parasitic infections in macrophages and mice is undisputed, whether Slc11a2 also contributes to antimicrobial functions is unknown. Our general hypothesis is that intracellular metal ion availability differs in epithelial cells and phagocytes in the gut due to the distinct cell-type expression of SLC11A1 and SLC11A2. First, we will use microbial biosensors of metal ion concentrations to infect bovine ligated ileal loops, a highly relevant model of enteric salmonellosis in humans, and map the spatiotemporal distribution of metal ion deprivation in the gut. Second, we will define whether SLC11A2, the sole NRAMP family member expressed in intestinal epithelial cells, defends against invading pathogens via metal nutrient limitation. Completion of this proposal will close a significant knowledge gap about host-microbe competition for metal ions in the gut during enteric infection.
项目总结

项目成果

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Leigh Knodler其他文献

Leigh Knodler的其他文献

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{{ truncateString('Leigh Knodler', 18)}}的其他基金

A bacterial biosensor for intracellular metal availability in the gut
用于肠道内细胞内金属可用性的细菌生物传感器
  • 批准号:
    10763683
  • 财政年份:
    2022
  • 资助金额:
    $ 5.85万
  • 项目类别:
A bacterial biosensor for intracellular metal availability in the gut
用于肠道内细胞内金属可用性的细菌生物传感器
  • 批准号:
    10764453
  • 财政年份:
    2022
  • 资助金额:
    $ 5.85万
  • 项目类别:
Epithelial intrinsic inflammasomes direct host defense against gut microbes
上皮内在炎症小体直接引导宿主防御肠道微生物
  • 批准号:
    10801424
  • 财政年份:
    2018
  • 资助金额:
    $ 5.85万
  • 项目类别:
Epithelial intrinsic inflammasomes direct host defense against gut microbes
上皮内在炎症小体直接引导宿主防御肠道微生物
  • 批准号:
    10202411
  • 财政年份:
    2018
  • 资助金额:
    $ 5.85万
  • 项目类别:
Epithelial intrinsic inflammasomes direct host defense against gut microbes
上皮内在炎症小体直接引导宿主防御肠道微生物
  • 批准号:
    10428520
  • 财政年份:
    2018
  • 资助金额:
    $ 5.85万
  • 项目类别:

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