Mechanism of Action of Sec1p-like Proteins in Membrane Trafficking

Sec1p 样蛋白在膜运输中的作用机制

• 批准号: nhmrc : 102541
• 负责人: Nia Bryant
• 金额: $ 15.67万
• 依托单位: Garvan Institute of Medical Research
• 依托单位国家: 澳大利亚
• 项目类别: NHMRC Project Grants
• 财政年份: 2000
• 资助国家: 澳大利亚
• 起止时间: 2000-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/mechanism-action-sec1p-membrane-trafficking/78037
• 关键词: Mechanism; Action; Sec1p; like; Proteins

One of the most important evolutionary changes that has occurred is the development of intracellular compartments. All eukaryotic cells possess numerous membrane-encased structures which provide the basis for intracellular specialisation. For example, in order to degrade unwanted components cells have developed degradative enzymes. It is vital for the cell that these enzymes are sequestered away from other cellular components to avoid destruction of valuable molecules. In addition, the cell has developed a complex assembly line of modifications that are added to proteins in a specific order as they travel to their final destination within the cell. This necessitates the accurate passage of molecules between compartments, a process known as vesicle transport. To orchestrate the complex network of vesicular transport steps between all of the various intracellular compartments it is necessary to employ complex machinery to guide and check that these steps occur with high fidelity. The goal of our research proposal is to define the function of one of the molecules involved in this control process, the so-called Sec1p proteins. The strength of our proposal lies in the diversity of our approach. We intend to explore the molecular advantages of a relatively simple eukaryotic organism, a yeast cell, and apply the findings obtained from this cell to a more complex but highly related vesicular transport process; that of the insulin-regulated movement of a glucose transporter in mammalian fat and muscle cells. While we intend to apply our findings to the treatment of patients with diabetes, it is our ultimate goal to be able to learn more about this fundamental cell biological process so that we can apply our knowledge to understanding many different disease states.

已经发生的最重要的进化变化之一是细胞内间隔的发展。所有真核细胞都有许多被膜包裹的结构，这为细胞内的特化提供了基础。例如，为了降解不需要的成分，细胞开发了降解酶。对于细胞来说，将这些酶与其他细胞成分隔离开来，以避免破坏有价值的分子，这是至关重要的。此外，细胞还开发了一条复杂的修饰装配线，当蛋白质到达细胞内的最终目的地时，这些修饰会以特定的顺序添加到蛋白质中。这就需要分子在隔室之间准确通过，这一过程被称为囊泡运输。为了协调所有不同细胞内隔室之间的囊泡运输步骤的复杂网络，有必要使用复杂的机械来指导和检查这些步骤是否高保真地发生。我们的研究计划的目标是确定参与这一控制过程的分子之一的功能，即所谓的Sec1p蛋白。我们建议的优势在于我们方法的多样性。我们打算探索一种相对简单的真核生物--酵母细胞的分子优势，并将从该细胞获得的发现应用于更复杂但高度相关的囊泡运输过程；即哺乳动物脂肪和肌肉细胞中胰岛素调节的葡萄糖转运体的运动。虽然我们打算将我们的发现应用于糖尿病患者的治疗，但我们的最终目标是能够更多地了解这一基本的细胞生物学过程，以便我们能够将我们的知识应用于了解许多不同的疾病状态。