The role of hemocytes in mosquito arbovirus infection

血细胞在蚊子虫媒病毒感染中的作用

• 批准号: 10200006
• 负责人: Ryan C. Smith
• 金额: $ 18.12万
• 依托单位: IOWA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10200006
• 关键词: Address; Aedes; Antiviral Agents; Arbovirus Infections; Arboviruses; Biology; Bite; Blood; Cells; Chemicals; Chikungunya fever; Culicidae; Data; Dengue; Dengue Fever; Dengue Virus; Disease; Drosophila genus; Economic Burden; Ensure; Environment; Goals; Hemocytes; Hemolymph; Human; Immune; Immune response; Infection; Innate Immune System; Interruption; Invaded; Laboratories; Mediating; Mediator of activation protein; Midgut; Morbidity - disease rate; Mosquito-borne infectious disease; Oral; Outcome; Pathway interactions; Phagocytes; Population; Positioning Attribute; Process; RNA Interference; Role; Salivary Glands; Sentinel; Site; Techniques; Testing; Tissues; Tropism; Viral; Virus; Virus Diseases; Virus Replication; Yellow Fever; ZIKA; ZIKV infection; Zika Virus; antiviral immunity; base; chemical genetics; combat; disease transmission; experience; experimental study; extracellular vesicles; genetic approach; health economics; inhibitor/antagonist; insight; mortality; pathogen; response; reverse genetics; tissue tropism; transmission process; uptake; vector competence; vector mosquito; viral transmission; virus tropism; zika fever

PROJECT SUMMARY Mosquito arbovirus transmission causes hundreds of millions of human infections every with significant disease morbidity and mortality outcomes. However, despite the requirements of the mosquito host for virus replication and transmission, our understanding of these mechanisms is poorly understood. This includes the role of mosquito immune cells, or hemocytes, which may have both positive and negative influences on virus replication. Building on our expertise in hemocyte biology and host-pathogen interactions, we now look to examine the role of mosquito hemocytes on dengue (DENV) and Zika virus (ZIKV) infection. Using a chemical genetics approach, we provide preliminary data demonstrating our ability to deplete phagocytic immune cell populations in Aedes aegypti, which serve as the basis of our proposed experiments. Therefore, the studies in this proposal will examine the contributions of phagocytic immune cells on DENV and ZIKV infections, and explore the mechanisms by which these cells may mediate systemic RNAi responses in the mosquito host (Aim 1). In addition, we will also explore potential roles of hemocytes as a secondary site of virus amplification that may increase viral titers and aid dissemination to increase the likelihood of transmission (Aim 2). Together, we expect that the results of this study will provide new information into the mechanisms of virus replication in the mosquito host and define the integral role of hemocytes in mosquito vector competence to arbovirus infection.

项目总结

项目成果

Frequency matters: How successive feeding episodes by blood-feeding insect vectors influences disease transmission.

• DOI: 10.1371/journal.ppat.1009590
• 发表时间: 2021-06
• 期刊: PLoS pathogens
• 影响因子: 6.7
• 作者: Brackney DE;LaReau JC;Smith RC
• 通讯作者: Smith RC