权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Design and Analysis of Human Gene Mapping Studies

人类基因图谱研究的设计与分析

基本信息

批准号：
10200112
负责人：
MICHAEL L BOEHNKE
金额：
$ 48.93万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-07 至 2023-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10200112
关键词：
Address Base Sequence Biological Biology Bipolar Disorder Catalogs Chromosome Mapping Complex Genetic Trait Computer Simulation Computer software Computing Methodologies Consensus DNA DNA Sequence Data Data Set Development Disease Equilibrium Etiology Frequencies Funding Gene Frequency Genes Genetic Genetic Variation Genetic study Genome Genotype Genotype-Tissue Expression Project Goals Health Human Human Gene Mapping Human Genetics Human Genome Human Genome Project Human Resources Lead Linkage Disequilibrium Methods Michigan Minor Non-Insulin-Dependent Diabetes Mellitus Population Problem Sets Problem Solving Production Quality Control RNA Research Research Design Research Personnel Resources Sampling Schizophrenia Sequence Analysis Software Tools Statistical Methods Structure Test Result Testing Time Trans-Omics for Precision Medicine Variant base causal variant computer data analysis computerized tools cost cost effective data resource design disease classification disorder prevention disorder risk epigenomics exome sequencing experimental study genetic testing genetic variant genome sequencing genome wide association study human disease improved insight method development novel novel therapeutics open source risk prediction risk stratification success targeted treatment tool trait transcriptome sequencing web services

项目摘要

The Human Genome Project and follow-on projects such as 1000 Genomes, GTEx, ENCODE, and TOPMed provide powerful resources to identify genes that influence human health and disease and variability in disease-related quantitative traits (QTs). Along with these resources have come increasingly efficient tools to genotype, sequence, and annotate the genome, and to support computation across these data. These resources and tools will be critical as we continue to explore the genetic basis of human disease and disease-related QTs. In this proposal, we describe statistical and computational problems that arise in human gene mapping, with a particular focus on sequence analysis, genotype imputation, and quality control. We describe statistical methods to address these problems and software tools and web services to facilitate their use. We will test resulting methods, tools, and web services via computer simulation and analysis of data from complex trait genetics studies in which we are involved. Specifically, we will: (1) develop tools to detect and estimate DNA sample contamination that are agnostic to genetic ancestry; (2) develop a test for Hardy-Weinberg equilibrium of sequence-based or imputed genotypes in the presence of population structure and robust to sample contamination; (3) enable more accurate variant filtering and genotype calling from DNA sequence data in the presence of population structure and/or sample contamination; (4) develop methods to detect sample contamination in RNA- and epigenomic sequence data; (5) extend the Michigan Imputation Server (MIS) to increase power of a sequence-based association studies by supporting use of external controls from existing sequence data resources, augmenting an existing imputation reference panel with the investigator's sequenced samples, and checking for contamination; and (6) document, distribute, and support efficient software tools to support these methods. Under separate funding, we will apply the resulting methods to help understand the genetic basis of type 2 diabetes and related QTs, and of schizophrenia and bipolar disorder. Success in these aims will enable more rapid identification of variants that predispose to human disease and account for variability in disease-related QTs, and has the potential to lead to new insights into basic biology and disease etiology, identify novel therapies, improve targeting of therapies, assist in disease classification, and support more accurate disease risk prediction. The modest cost of statistical and computational methods development, and the impact of these methods across many studies, makes our proposed research highly cost effective.

人类基因组计划和后续项目，如1000基因组、GTEx、ENCODE和TOPMed