The biophysical roles of hair cell and calyx afferent ion channels in synaptic transmission between vestibular receptors and afferent nerves in mammals.

毛细胞和花萼传入离子通道在哺乳动物前庭受体和传入神经之间突触传递中的生物物理作用。

• 批准号: 10201561
• 负责人: Donatella Contini
• 金额: $ 15.99万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-15 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10201561
• 关键词: AMPA Receptors; Acceleration; Action Potentials; Address; Anterior; Architecture; Area; Autonomic nervous system disorders; Biological; Biophysics; Cells; Closure by clamp; Complex; Coupling; Development; Disease; Dizziness; Electrodes; Elements; Environment; Epithelial; Equilibrium; Exposure to; Face; Frequencies; Functional disorder; Future; Generations; Geometry; Glutamates; Hair Cells; House mice; In Situ; Ion Channel; Ions; Mammals; Mechanical Stimulation; Mechanics; Membrane Potentials; Microscopic; Mus; Neurotransmitters; Organ; Perilymph; Periodicity; Peripheral; Physiology; Potassium; Preparation; Property; Receptor Cell; Research; Risk; Role; Semicircular canal structure; Sensory Hair; Stimulus; Structure; Synapses; Synaptic Cleft; Synaptic Transmission; System; Testing; Turtles; Type I Hair Cell; Type II Hair Cell; Variant; Vertigo; afferent nerve; base; biophysical properties; cell envelope; cell type; driving force; experience; experimental study; extracellular; genetic manipulation; in vivo; innovation; otoconia; postsynaptic; quantum; rate of change; receptor; reconstruction; response; signal processing; transmission process; voltage

Project Summary Prior vestibular research has shown that afferent responses from semicircular canals and otolith organs deviate from the coherent mechanical stimulation imparted by the overlying accessory structures. This suggests further signal processing by hair cells (HCs) and primary afferent conductances, and by the HC–afferent synapse. Processing is complicated by the parallel modes of synaptic transmission between HCs and afferents, and the convergence of multiple HCs onto single afferents. Type I HCs are enveloped by an afferent calyx, creating a cup-shaped cleft between the two elements. By contrast, type II HCs synapse onto bouton endings and/or the external face of a calyx, with relatively small areas of cellular apposition. Further complexity is conferred by three classes of HC-to-afferent convergence. In the simplest configuration, HCs converge onto an afferent solely via bouton endings. Increased complexity is found at calyceal endings, either as simple calyces enveloping a single HC or as complex calyces where the afferent encompasses two or more HCs. The highest complexity occurs at dimorphic endings that contact both type I and II HCs via a combination of bouton and inner– and outer–face calyceal synapses. Prior experiments in turtles have shown that for calyceal endings, rapid excitatory synaptic transmission, via glutamatergic AMPA receptors, is modulated by K+, H+, and Ca2+ accumulation. In response to HC depolarization, there are dynamic changes in ion concentration in the cleft. These in turn impact responses in both the type I HCs and their afferents due to changes in the equilibrium potentials and driving forces for conductances facing the cleft. As a result, properties of these calyceal contacts are significantly different from those for HC and afferent conductances bathed in the bulk perilymph. Consequently, prior single-electrode biophysical experiments on either HCs or their afferents in situ, or using isolated cells, have been unable to dissect the contributions of HCs and afferents resulting from reciprocal interactions created by the unique volume of the synaptic cleft coupling the two. I now have preliminary biophysical results on isolated anterior semicircular canal epithelia in the mouse, Mus musculus. These experiments demonstrate that I will be able to extend and refine the development of a mammalian preparation in which I can characterize the ionic environment of the synaptic cleft and the biophysical characteristics of synaptic transmission between HCs and afferents under conditions where the membrane potentials of the HC and its associated afferent are controlled simultaneously. I will focus on two areas: (1) the gating of type I HC conductances exposed to the dynamic environment of the synaptic cleft; and (2) the integration of synaptic inputs from type I HCs on the internal face of the calyx and type II HCs synapsing either on the external face of the calyx, or on bouton endings of dimorphic afferents.

项目总结