Noninvasive Pesticide Biomonitoring Using Sweat Patches

项目总结 长期低剂量接触农用杀虫剂被广泛认为是对 农场工人。不幸的是，描述农场工人和农民之间农药暴露差异的来源 农药暴露的长期后果受到测量挑战的阻碍，特别是 缺乏一种简单、非侵入性的农药生物监测策略。汗斑提供了一种令人信服的 在实地研究中评估农药暴露的替代方法。有证据表明，农药代谢物 是在汗液中发现的，汗片现在被常规用于药物监测研究。这样做的目的是 跨学科研究是为了确定使用汗片进行农药暴露生物监测的可行性。 在拉丁裔农场工人中。我们将检验这样一种假设，即汗斑采样将允许敏感和 具有高合规率的一致测量。这项可行性研究的目的是：1)开发和 评估用于测量农药及其代谢物的实验室技术的准确性和精密度 从汗斑中提取，2)确定杀虫剂和 从汗液中获得的代谢物到从尿液中获得的代谢物。次要目标是记录挑战和 农场工人在使用(如打开、涂抹、佩戴、拆卸和储存)中遇到的问题 汗斑。与传统尿液筛查相比，汗贴的优点包括：贴片是非侵入性的， 显示出更高的合规性，并降低了该领域未来研究的财务成本。我们建议的工作 涉及实验室和现场程序的组合，随后的集成将导致 对职业健康有重大影响的高度翻译性项目。

PROJECT SUMMARY Chronic low-dose exposure to agricultural pesticides is widely accepted as a fundamental health threat to farmworkers. Unfortunately, delineating sources of variation in pesticide exposure among farmworkers and the long-term consequences of pesticide exposure is hampered by measurement challenges, particularly the absence of an easy, nonintrusive strategy for pesticide biomonitoring. Sweat patches offer a compelling alternative for assessing exposure to pesticides in field studies. Evidence indicates that pesticide metabolites are found in sweat, and sweat patches are being used routinely now in drug monitoring studies. The goal of this interdisciplinary study is to determine the feasibility of using sweat patches for biomonitoring pesticide exposure among Latino farmworkers. We will test the hypothesis that sweat patch sampling will allow for sensitive and consistent measurements with a high rate of compliance. The aims of this feasibility study are to: 1) develop and assess the accuracy and precision of laboratory techniques used to measure pesticides and their metabolites extracted from sweat patches and 2) determine the levels and variance in concentrations of pesticides and metabolites obtained from sweat to those obtained from urine. A secondary aim is to document challenges and problems experienced by farmworkers in using (e.g., opening, applying, wearing removing, and storing) the sweat patches. Advantages of sweat patches to traditional urine screening include the patch being noninvasive, showing increased compliance, and reducing financial cost for future research in this field. Our proposed work involves a combination of both laboratory and field procedures, and the subsequent integration will result in a highly translational project with substantial occupational health impact.