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HCMV-induced innate-like CD8 T cells and allogeneic HCT outcome

HCMV 诱导的先天样 CD8 T 细胞和同种异体 HCT 结果

基本信息

批准号：
10390447
负责人：
KATHARINE C HSU
金额：
$ 70.98万
依托单位：
SLOAN-KETTERING INST CAN RESEARCH
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-04-09 至 2026-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10390447
关键词：
Acute Myelocytic Leukemia Adopted Allogenic Blood Bone Marrow Transplantation CD28 gene CD3 Antigens CD8-Positive T-Lymphocytes CD8B1 gene Cell Line Cell Therapy Cells Clonality Collaborations Cytomegalovirus Cytotoxic T-Lymphocytes Development Down-Regulation Dysmyelopoietic Syndromes Epigenetic Process Event Exhibits Exposure to Fibroblasts Gene Expression Profile Genetic Genetic Transcription Goals Human Immune Immune response Immunologic Surveillance Immunosuppression In Vitro Individual Infection Investigation KLRD1 gene Leprosy Leukocytes Link Lymphocyte Lymphoid Lymphoid Cell Malignant Neoplasms Measurable Measures Memory Molecular Morbidity - disease rate Natural Killer Cells Organ Outcome Pathway interactions Patients Penetrance Peptides Phenotype Population Relapse Risk Sampling Signal Transduction Solid Source Specificity Surface T cell response T-Cell Development T-Lymphocyte Therapeutic Thymus Gland Transplant Recipients Viral Viral Physiology Virus Virus Diseases acute myeloid leukemia cell alpha-beta T-Cell Receptor beneficiary cancer cell clinical database curative treatments cytotoxic hematopoietic cell transplantation immune reconstitution imprint international center leukemia leukemia relapse member mortality neonate pathogen post-transplant programmed cell death protein 1 programs receptor recruit response seropositive transcription factor transcriptome sequencing tumor γδ T cells

项目摘要

PROJECT SUMMARY Human cytomegalovirus (HCMV) infects all populations with a penetrance of 50-100% and is kept latent by innate and adaptive surveillance. However, it is a significant cause of morbidity and mortality in conditions of immune reconstitution and suppression, such as in neonates and recipients of solid organ or hematopoietic cell transplants. The T cell response to HCMV through classical HCMV peptide-specific αβ cytotoxic T lymphocytes has been well-studied, and the development of NKG2C+ natural killer cells in response to HCMV infection and reactivation is under active investigation. In addition to these lymphocytes, however, large populations of αβ- TCR CD8 T cells that express NKG2C and other NK-associated receptors have also been observed in HCMV- seropositive healthy donors and patients. These innate-like NKG2C+ CD8 T cells appear to have broad activity against AML and HCMV-infected cells, no activity against uninfected allogeneic fibroblasts, and reduced expression of PD-1 in response to CD3 stimulation. RNAseq analysis has revealed that NKG2C+ CD8 T cells have reduced expression of the transcription factor Bcl11b, critical for cutting off alternative innate fates during the early thymic development of T cells. The central hypothesis of this proposal is that HCMV exposure induces an NKG2C+ CD8 T cell population by diverting clonotypic T cells toward an innate fate through the downregulation of Bcl11b, which alters TCR signaling and promotes alternative recognition pathways beneficial to leukemia patients. The first aim of the proposal is to evaluate the T cell identity of members of the NKG2C+ CD8 T cell population (clonality, TCR specificity and signaling) and how their transcriptional and epigenetic programs are altered from other CD8 T cells by Bcl11b loss. The second aim will assess the function of the NK- associated activating and inhibitory receptors on the NKG2C+ CD8 T cells, with the goal of identifying the mechanism behind their anti-tumor and anti-HCMV activity. Finally, in a collaboration with the Center for International Blood and Marrow Transplantation, an extensive hematopoietic cell transplantation patient sample bank and clinical database will be utilized to determine whether the post-transplantation emergence of an NKG2C+ CD8 T cell population impacts the risk of leukemia relapse and overall survival. Together, the results of these studies will elucidate not only the therapeutic potentials of this innate-like T cell population but also how adaptive and innate fates can be bridged.

项目总结