The role of epigenetic modifiers in regulating the developmental plasticity of cranial neural crest cells
表观遗传修饰剂在调节颅神经嵴细胞发育可塑性中的作用
基本信息
- 批准号:10211467
- 负责人:
- 金额:$ 22.77万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-04-01 至 2023-03-31
- 项目状态:已结题
- 来源:
- 关键词:ATAC-seqBinding SitesCartilageCell Differentiation processCell LineageCellsCephalicChondrocytesChromatinChromatin Remodeling FactorCompetenceCongenital AbnormalityDNA BindingDevelopmentDevelopmental BiologyDifferentiated GeneEVI1 geneEmbryoEpigenetic ProcessEtiologyExhibitsGene ExpressionGenesGeneticGenetic TranscriptionGenomicsGoalsGrowthHumanKnowledgeLinkMissionMolecularNatureNeural CrestNeural Crest CellNeurogliaNeuronal DifferentiationNeuronsPeripheral Nervous SystemProtein FamilyProteinsPublic HealthRegenerative MedicineRegulationRegulator GenesResearchRoleSeriesSignal TransductionSiteSystemTestingTimeTissuesTranscriptional RegulationTrigeminal SystemUnited States National Institutes of HealthXCL1 geneZebrafishbonecartilage cellcell typechromatin modificationcraniofacialcraniofacial tissuedevelopmental plasticitydisabilityhistone methyltransferasehistone modificationinsightloss of functionmigrationmorphogensmultipotent cellmutantneuromechanismprogenitorrecruitrepairedsingle cell sequencingsingle-cell RNA sequencingstemstem cellstissue repairtranscription factortranscriptometranscriptome sequencing
项目摘要
Summary
How stem progenitor cells maintain plasticity for proper cell fate determination over developmental time is a
fundamental question in developmental biology and regenerative medicine. Cranial neural crest cells (cNCCs)
are an excellent example of a well defined cellular lineage transition in which multipotent cells step through a
series of more restricted progenitors to give rise to diverse array of differentiated cell types, including neurons
and glia of the peripheral nervous system as well as craniofacial cartilage and bone. Thus, understanding the
genetic and epigenetic regulators in cNCC development is key to understanding how cell fate is determined as
well as how cells can be reprogrammed. We hypothesize that the cNCC cartilage/neuron/glial progenitor
retains plasticity through developmental time and cNCC fate acquisition is controlled by regulation of
chromatin accessibility by prdm3. The rationale for the proposed studies is that an in-depth understanding
of the specific factors involved in cNCC lineage transitions will provide insights into both normal developmental
plasticity of cNCCs as well as how progenitors can be reprogramed for tissue repair. We will test this
hypothesis in the following specific aims: 1) Test the hypothesis that prdm3 acts as a molecular cell fate
switch during cNCC differentiation. Here we will test the hypothesis that prdm3 activity is required in cNCCs
cell autonomously to promote the temporal recruitment of progenitors to cartilage by repressing neuronal cell
fate. 2) Test the hypothesis that the cartilage/neuronal/glial (CNG) progenitor retains plasticity through
developmental time and can be reprogramed by loss of prdm3. In Aim 2, hypothesis that CNG
progenitors retain plasticity over developmental time and into larval stages and are reprogramed with loss of
prdm3. 3) Test the hypothesis that Prdm3 regulates the timing of cNCC differentiation by controlling of
genomic accessibility. In Aim 3, we will test the hypothesis that loss of Prdm3 leads to global alterations in
chromatin state at cNCC progenitor genes, which in turn controls the timing of differentiation. Together, these
studies will reveal basic information of how cNCCs differentiate into specific cell types during development.
The results of this proposal have the potential to reveal important new insights into normal developmental
plasticity of cNCCs such that tissue reprograming can be developed for the repair of damaged craniofacial
tissues.
总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
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Kristin Artinger其他文献
Kristin Artinger的其他文献
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{{ truncateString('Kristin Artinger', 18)}}的其他基金
The role of epigenetic modifiers in regulating the developmental plasticity of cranial neural crest cells
表观遗传修饰剂在调节颅神经嵴细胞发育可塑性中的作用
- 批准号:
10805033 - 财政年份:2023
- 资助金额:
$ 22.77万 - 项目类别:
Reprogramming myogenic regulatory factors in RMS to promote differentiation and halt growth
重新编程 RMS 中的生肌调节因子以促进分化并阻止生长
- 批准号:
10682281 - 财政年份:2023
- 资助金额:
$ 22.77万 - 项目类别:
Genetic and epigenetic regulation of cranial neural crest differentiation
颅神经嵴分化的遗传和表观遗传调控
- 批准号:
10817293 - 财政年份:2023
- 资助金额:
$ 22.77万 - 项目类别:
Genetic and epigenetic regulation of cranial neural crest differentiation
颅神经嵴分化的遗传和表观遗传调控
- 批准号:
10316019 - 财政年份:2021
- 资助金额:
$ 22.77万 - 项目类别:
The role of epigenetic modifiers in regulating the developmental plasticity of cranial neural crest cells
表观遗传修饰剂在调节颅神经嵴细胞发育可塑性中的作用
- 批准号:
10352461 - 财政年份:2021
- 资助金额:
$ 22.77万 - 项目类别:
Genetic and epigenetic regulation of cranial neural crest differentiation
颅神经嵴分化的遗传和表观遗传调控
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10442617 - 财政年份:2021
- 资助金额:
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9906909 - 财政年份:2019
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The mechanisms regulating actin dynamics and polarized membrane transport during cell migration
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The mechanisms regulating actin dynamics and polarized membrane transport during cell migration
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10693336 - 财政年份:2018
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$ 22.77万 - 项目类别:
Function of chromatin modifiers in cranial neural crest development
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8913662 - 财政年份:2015
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