Whispering Gallery Mode Devices for the Rapid Detection of Pan-Filoviruses

项目摘要 丝状病毒，包括埃博拉病毒、马尔堡病毒和库瓦病毒，是一类具有 对健康的巨大影响。丝状病毒与非洲的许多疫情有关，包括 刚果民主共和国(刚果(金))持续的疫情，作为生物恐怖主义媒介的潜在风险，以及 向美国等非流行国家传播的潜在风险。在这两个方面都很关键 对暴发的管理以及对感染患者的治疗是特定和快速的诊断测试。 尽管这些病原体构成了严重的健康风险，但目前利用聚合酶链式反应和 ELISA有很大的局限性，包括需要集中式实验室、冷链保管和 在感染早期阶段诊断能力有限。这项提案旨在填补这一空白，并利用高度 敏感型光学传感器，用于快速检测丝状病毒 糖蛋白(GP)和埃博拉病毒可溶性糖蛋白(SGP)是丝状病毒的两个高度敏感的生物标志物。 WGM传感器是一种独特的光学设备，其中的光被限制在小体积内，因此领先 信号响应的巨大增强。除了它们的灵敏度之外，这些设备还提供 制造成本低廉且可扩展的优势，能够与传统 电子学，以及多路测量的可能性。与这些装置结合的将是抗体 对抗丝状病毒gp和sgp，后者是一种非常强大的前哨生物标志物，从几个小时到几天呈阳性 在感染者出现症状之前。这种学术和产业的伙伴关系将迅速发展。 以及对丝状病毒gp和sgp的敏感诊断试验，为后续的开发奠定了基础 将这项技术转化为一种强大的、可现场部署的设备，用于丝状病毒检测。

Project Summary Filoviruses, including the Ebola viruses, Marburg viruses, and Cueva virus, are a class of pathogens with enormous health implications. Filoviruses have been implicated in numerous outbreaks in Africa, including the ongoing outbreak in the Democratic Republic of Congo (DRC), potential risk as an agent of bioterrorism, and the potential risk of transmission to non-endemic countries such as the United States. Critical in both the management of outbreaks as well as the treatment of infected patients are specific and rapid diagnostic tests. Despite the critical health risk posed by these pathogens, current diagnostic techniques utilizing PCR and ELISAs have significant limitations, including the need for centralized laboratories, cold-chain custody, and limited diagnostic ability in the early stages of infection. This proposal seeks to fill this gap and leverage a highly sensitive class of optical sensors, whispering gallery mode (WGM) devices, for the rapid detection of filovirus glycoproteins (GP) and the ebolavirus soluble glycoprotein (sGP), two highly sensitive biomarkers for filoviruses. WGM sensors are a unique class of optical devices in which light is confined to a small volume, therefore leading to an enormous enhancement in signal response. In addition to their sensitivity, these devices also offer the advantages of inexpensive and scalable fabrication costs, the ability to be integrated with conventional electronics, and the potential for multiplexed measurements. In combination with these devices will be antibodies against filovirus GP and sGP, the latter an enormously powerful sentinel biomarker that is positive hours to days before presentation of symptoms in infected individuals. This academic-industry partnership will develop a rapid and sensitive diagnostic test for filovirus GP and sGP, and subsequently lay the foundation for the developing this technology into a powerful, field-deployable device for filovirus detection.