Whispering Gallery Mode Devices for the Rapid Detection of Pan-Filoviruses

项目摘要 丝状病毒，包括埃博拉病毒、马尔堡病毒和奎瓦病毒，是一类病原体， 巨大的健康影响。丝状病毒与非洲的许多疫情有关，包括 刚果民主共和国（DRC）正在爆发的疫情，作为生物恐怖主义的潜在危险，以及 潜在的风险传播到非流行国家，如美国。关键在于， 控制疾病爆发以及治疗受感染的病人都是特异和快速的诊断测试。 尽管这些病原体造成了严重的健康风险，但目前的诊断技术利用PCR和 ELISA有很大的局限性，包括需要集中实验室，冷链监管， 感染早期诊断能力有限。这项建议旨在填补这一空白，并利用一个高度 灵敏的光学传感器，回音壁模式（WGM）设备，用于快速检测丝状病毒 糖蛋白（GP）和埃博拉病毒可溶性糖蛋白（sGP），这是丝状病毒的两种高度敏感的生物标志物。 WGM传感器是一类独特的光学器件，其中光被限制在小体积内，因此领先于 信号响应的巨大增强。除了灵敏度，这些设备还提供 便宜和可扩展的制造成本的优点，与传统的集成的能力， 电子学和多路复用测量的潜力。与这些设备相结合的将是抗体 针对丝状病毒GP和sGP，后者是非常强大的哨兵生物标志物，在数小时至数天内呈阳性 在感染者出现症状之前。这种学术界与工业界的合作关系将迅速发展， 建立了丝状病毒GP和sGP的敏感诊断方法，为进一步开发丝状病毒GP和sGP的诊断试剂奠定了基础。 将这项技术转化为一种强大的、可现场部署的丝状病毒检测设备。

Project Summary Filoviruses, including the Ebola viruses, Marburg viruses, and Cueva virus, are a class of pathogens with enormous health implications. Filoviruses have been implicated in numerous outbreaks in Africa, including the ongoing outbreak in the Democratic Republic of Congo (DRC), potential risk as an agent of bioterrorism, and the potential risk of transmission to non-endemic countries such as the United States. Critical in both the management of outbreaks as well as the treatment of infected patients are specific and rapid diagnostic tests. Despite the critical health risk posed by these pathogens, current diagnostic techniques utilizing PCR and ELISAs have significant limitations, including the need for centralized laboratories, cold-chain custody, and limited diagnostic ability in the early stages of infection. This proposal seeks to fill this gap and leverage a highly sensitive class of optical sensors, whispering gallery mode (WGM) devices, for the rapid detection of filovirus glycoproteins (GP) and the ebolavirus soluble glycoprotein (sGP), two highly sensitive biomarkers for filoviruses. WGM sensors are a unique class of optical devices in which light is confined to a small volume, therefore leading to an enormous enhancement in signal response. In addition to their sensitivity, these devices also offer the advantages of inexpensive and scalable fabrication costs, the ability to be integrated with conventional electronics, and the potential for multiplexed measurements. In combination with these devices will be antibodies against filovirus GP and sGP, the latter an enormously powerful sentinel biomarker that is positive hours to days before presentation of symptoms in infected individuals. This academic-industry partnership will develop a rapid and sensitive diagnostic test for filovirus GP and sGP, and subsequently lay the foundation for the developing this technology into a powerful, field-deployable device for filovirus detection.