Whispering Gallery Mode Devices for the Rapid Detection of Pan-Filoviruses

用于快速检测泛丝状病毒的回音壁模式装置

• 批准号: 10081794
• 负责人: Gaya K. Amarasinghe
• 金额: $ 29.99万
• 依托单位: INTEGRATED BIOTHERAPEUTICS, INC.
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-06 至 2022-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10081794
• 关键词: Africa; Angola; Antibodies; Biological; Biological Markers; Bioterrorism; Biotin; Buffers; Case Fatality Rates; Cells; Cessation of life; Characteristics; Chemistry; Chiroptera; Cold Chains; Communicable Diseases; Complex; Containment; Country; Coupled; Coupling; Democratic Republic of the Congo; Detection; Development; Devices; Diagnosis; Diagnostic; Diagnostic Procedure; Diagnostic tests; Disease; Disease Outbreaks; Early Diagnosis; Ebola Hemorrhagic Fever; Ebola virus; Electronics; Elements; Ensure; Enzyme-Linked Immunosorbent Assay; Family; Fatality rate; Filoviridae Infections; Filovirus; Foundations; Frankfurt-Marburg Syndrome Virus; Gene Expression; Generations; Glycoproteins; Hand; Health; Hour; Human; Individual; Industry; Infection; Laboratories; Light; Liquid substance; Marburg Virus Disease; Measurement; Membrane Glycoproteins; Methods; Microbubbles; Monoclonal Antibodies; Nature; Netherlands; Nucleic Acid Amplification Tests; Optics; Pathogen detection; Patients; Performance; Phase; Process; RNA Viruses; Recovery; Resources; Risk; Running; Sampling; Sentinel; Serum; Signal Transduction; Silanes; Solid; Specificity; Streptavidin; Surface; Symptoms; Technology; Testing; Time; Uganda; United States; Viral Hemorrhagic Fevers; Virulence; Virus; Virus Diseases; Virus Replication; Western Africa; Work; Zaire Ebola virus; Zoonoses; base; cost; design; diagnostic assay; global health; hemorrhagic fever virus; industry partner; nanoparticle; new technology; optical sensor; pathogen; rapid detection; rapid diagnosis; response; sensor; single molecule; therapy development; transmission process; weapons

Project Summary Filoviruses, including the Ebola viruses, Marburg viruses, and Cueva virus, are a class of pathogens with enormous health implications. Filoviruses have been implicated in numerous outbreaks in Africa, including the ongoing outbreak in the Democratic Republic of Congo (DRC), potential risk as an agent of bioterrorism, and the potential risk of transmission to non-endemic countries such as the United States. Critical in both the management of outbreaks as well as the treatment of infected patients are specific and rapid diagnostic tests. Despite the critical health risk posed by these pathogens, current diagnostic techniques utilizing PCR and ELISAs have significant limitations, including the need for centralized laboratories, cold-chain custody, and limited diagnostic ability in the early stages of infection. This proposal seeks to fill this gap and leverage a highly sensitive class of optical sensors, whispering gallery mode (WGM) devices, for the rapid detection of filovirus glycoproteins (GP) and the ebolavirus soluble glycoprotein (sGP), two highly sensitive biomarkers for filoviruses. WGM sensors are a unique class of optical devices in which light is confined to a small volume, therefore leading to an enormous enhancement in signal response. In addition to their sensitivity, these devices also offer the advantages of inexpensive and scalable fabrication costs, the ability to be integrated with conventional electronics, and the potential for multiplexed measurements. In combination with these devices will be antibodies against filovirus GP and sGP, the latter an enormously powerful sentinel biomarker that is positive hours to days before presentation of symptoms in infected individuals. This academic-industry partnership will develop a rapid and sensitive diagnostic test for filovirus GP and sGP, and subsequently lay the foundation for the developing this technology into a powerful, field-deployable device for filovirus detection.

项目概要 丝状病毒，包括埃博拉病毒、马尔堡病毒和奎瓦病毒，是一类具有以下特征的病原体： 巨大的健康影响。丝状病毒与非洲的许多疫情爆发有关，包括 刚果民主共和国（DRC）持续爆发的疫情、作为生物恐怖主义媒介的潜在风险以及 传播到美国等非流行国家的潜在风险。在这两个方面都至关重要 疫情管理以及感染患者的治疗是具体而快速的诊断测试。 尽管这些病原体构成了严重的健康风险，但目前利用 PCR 和 ELISA 具有显着的局限性，包括需要集中实验室、冷链保管和 感染早期阶段的诊断能力有限。该提案旨在填补这一空白并利用高度 敏感级光学传感器、回音壁模式 (WGM) 设备，用于快速检测丝状病毒 糖蛋白（GP）和埃博拉病毒可溶性糖蛋白（sGP）是丝状病毒的两种高度敏感的生物标志物。 WGM 传感器是一类独特的光学设备，其中光被限制在很小的体积内，因此领先 信号响应的巨大增强。除了灵敏度之外，这些设备还提供 制造成本低廉且可扩展的优点，能够与传统技术集成 电子学，以及多重测量的潜力。与这些设备相结合的是抗体 针对丝状病毒 GP 和 sGP，后者是一种非常强大的哨兵生物标志物，在数小时至数天内呈阳性 在感染者出现症状之前。这种学术界与工业界的合作关系将迅速发展 并对丝状病毒GP和sGP进行灵敏的诊断检测，为后续的开发奠定基础 该技术已成为一种强大的、可现场部署的丝状病毒检测设备。