Novel VP30-host Interactions that Negatively Regulate Ebola Virus Infection

项目摘要/摘要 丝状病毒，埃博拉和马尔堡病毒(EBOV和MARV)，是一种新出现的负链RNA病毒 与严重病毒性出血热暴发有关。这些病毒的毒性和新出现的性质 人畜共患病原体使其成为对人类健康的重大威胁，潜在的生物恐怖主义因素，以及 NIAID A类优先病原体。父R01应用程序的总体目标是确定EBOV的特征 VP30(EVP30)，一种促进病毒转录的关键病毒蛋白，及其与宿主因子的相互作用。这 回应PA-21-071的补充要求--促进健康相关多样性的研究补充 研究(行政辅助-不允许临床试验)将支持受训者的培训和职业发展 目前参加了医学生培训计划，以发展她作为内科科学家的职业生涯。 扩展蛋白质相互作用和翻译后修饰的科学研究计划 研究将绘制丝状病毒蛋白的泛素化图谱。这些拟议的努力将增进我们的理解 丝状病毒与宿主之间的相互作用，并为职业发展提供机会。

Project Summary/Abstract The filoviruses, Ebola and Marburg viruses (EBOV and MARV), are emerging, negative-strand RNA viruses associated with outbreaks of severe viral hemorrhagic fever. The virulence and emerging nature of these zoonotic pathogens makes them a significant threat to human health, potential agents of bioterrorism, and NIAID category A priority pathogens. The overall goal of the parent R01 application is to characterize EBOV VP30 (eVP30), a key viral protein that facilitates viral transcription, and its interactions with host factors. This supplement request in response to PA-21-071 - Research Supplements to Promote Diversity in Health-Related Research (Admin Supp - Clinical Trial Not Allowed) will support the training and career development of trainee currently enrolled in a medical student training program to develop her career as a physician scientist. Scientific research program, which extends protein-protein interaction and post translational modification studies will map the ubiquitination of filoviral proteins. These proposed efforts will enhance our understanding of filoviral-host interactions and provide an opportunity for career development.