权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Stanford Tissue Mapping Center

斯坦福大学组织绘图中心

基本信息

批准号：
10213800
负责人：
GARRY P NOLAN
金额：
$ 167.55万
依托单位：
STANFORD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-19 至 2022-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10213800
关键词：
ATAC-seq Antibodies Architecture Area Atlases Back Biological Assay Blood Circulation Brain Death Cells Collection Colon Communities Complex Consent Data Data Set Detection Diarrhea Digestion Disease Duodenum Encyclopedia of DNA Elements Epitopes Food Gastrointestinal tract structure Genome Genomics Goals Health Heterogeneity Histologic Homeostasis Human Human BioMolecular Atlas Program Human body Imaging Techniques Immune response Immunity Individual Inflammatory Bowel Diseases Ingestion Intestines Large Intestine Length Maintenance Maps Mediating Metabolism Methodological Studies Methods Molecular Neurophysiology - biologic function Nutritional Organ Organ Donor Participant Phase Physiology Play Population Process Production Proteins Publications Research Personnel Resolution Role Sampling Shapes Small Intestines Structure System Techniques Technology Tissue Sample Tissues Vision absorption brain tissue cell motility data dissemination data sharing experience gut microbiome host microbiome human disease ileum indexing insight interest jejunum member microbiome nutrient absorption preservation scale up single cell analysis single-cell RNA sequencing

项目摘要

ABSTRACT: Overall The small bowel and colon are organs critical for maintaining homeostasis of the human body by mediating nutritional absorption upon the ingestion of food. Though both organs are extensive in length, there are known differences in function and cellular heterogeneity within different portions of each. Also, a cross section anywhere in the bowel reveals a complex layering of components involved in absorption and secretion, motility of gut contents, circulation, and immunity. In this submission, we propose the establishment of the Stanford Tissue Mapping Center (TMC) to map the complexity of the the small bowel and colon with cell-to-cell resolution in histologic sections, both along their lengths and across multiple individuals. To accomplish this goal, we will collect tissues from brain-dead organ donors with explicit consent for distribution among the HuBMAP consortium and open access genome data sharing (GDS). Two sets of technologies will then be employed. Tissue samples will be subjected to single cell (sc) ATAC-seq and scRNA-seq. These ‘omic profiles will then be spatially mapped back to histologic sections using a highly multiplexed system of antibody-tagged target epitopes, which we call CO-Detection by indEXing (CODEX). An integrative analysis will be performed to identify proteins important to establishing the identity of each cell population within the tissue.

摘要：总体小肠和结肠是维持人体内环境稳定的关键器官。在摄取食物时调节营养吸收。尽管这两个器官的长度都很长，每个细胞的不同部分在功能和细胞异质性方面都有已知的差异。另外，肠内任何一处的横切面显示了参与吸收的成分的复杂分层。以及分泌物、肠道内容物的运动、循环和免疫。在本意见书中，我们建议建立斯坦福组织测绘中心(TMC)以绘制小组织的复杂性肠和结肠组织切片中细胞对细胞的分辨率，无论是沿其长度还是横向多个个体。为了实现这一目标，我们将收集脑死亡器官捐赠者的组织明确同意在HuBMAP联盟之间分发和开放获取基因组数据共享 (GDS)。然后将采用两套技术。组织样本将接受单细胞培养 (Sc)atac-seq和scRNA-seq.然后，这些基因组图谱将被空间映射回组织学使用高度多元化的抗体标记的目标表位系统的部分，我们称之为索引联合检测(CODEX)。将进行综合分析以识别蛋白质对于确定组织内每个细胞群体的身份很重要。