MPM non-invasive imaging of biological interactions following drug delivery with micro-nanoprojection patches.

使用微纳米投影贴片给药后生物相互作用的 MPM 无创成像。

• 批准号: nhmrc : 456150
• 负责人: Prof Mark Kendall
• 金额: $ 26.91万
• 依托单位: The University of Queensland
• 依托单位国家: 澳大利亚
• 项目类别: NHMRC Project Grants
• 财政年份: 2007
• 资助国家: 澳大利亚
• 起止时间: 2007-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/mpm-non-invasive-nanoprojection-patches/82511
• 关键词: MPM; non; invasive; imaging; biological

The overarching aim of my research is to develop and evaluate effective, practical and reproducible physical methods for delivering genes and drugs to specific immunologically-sensitive cells in the skin to ultimately treat and vaccinate against human diseases. I recently patented a method using arrays of nano-scale projections on a patch to accurately, efficiently and safely deliver biomolecules not just to specific skin cells, but also to organelles within them. Conceptually, the delivery device is a set of microscopic nanoneedles coated with drug substance and applied to the skin as a small patch. The device is practical, needle-free and pain-free. The aim of this current project is to use the micro-nanoprojection array patches-configured to uniquely deliver biomolecules to cells within given strata-to find: 1) what delivery sites of antigen-expression plasmid- toll like receptor (TLR) agonist lead to strong humoral immune responses in the intact animal. 2) whether delivery of different TLR agonists have different effects on the maturation and migration of the different professional antigen presenting cells (APCs) in the skin, as visualised locally by Multi-Photon Microscopy (MPM). 3) whether differences in APC maturation and migration are associated with different systemic antibody responses. We will identify optimal delivery sites of drugs-vaccines to the skin (layer, cells targeted, duration of delivery) with MPM for desired systemic immune responses. This will have important contributions towards improving immunotherapeutics of major diseases via skin targeting with micro-nanoprojection array patch technologies (and other methods).

我的研究的首要目标是开发和评估有效，实用和可重复的物理方法，将基因和药物传递到皮肤中的特定免疫敏感细胞，以最终治疗和接种人类疾病。我最近申请了一种方法的专利，该方法使用贴片上的纳米级投影阵列，不仅可以准确，有效和安全地将生物分子传递到特定的皮肤细胞，还可以传递到细胞内的细胞器。从概念上讲，递送装置是一组涂覆有药物物质的微观纳米针，并作为小贴片施加到皮肤上。该装置实用、无针、无痛。本项目的目的是使用微-纳米突出物阵列贴片-被配置为独特地将生物分子递送到给定层内的细胞-以发现：1）抗原表达质粒-Toll样受体（TLR）激动剂的递送位点在完整动物中导致强烈的体液免疫应答。2)不同TLR激动剂的递送是否对皮肤中不同专职抗原呈递细胞（APC）的成熟和迁移具有不同的影响，如通过多光子显微镜（MPM）局部可见的。3)APC成熟和迁移的差异是否与不同的全身性抗体应答有关。我们将确定最佳的药物-疫苗的皮肤（层，细胞靶向，交付的持续时间）与MPM所需的全身免疫反应的交付网站。这将对通过使用微纳米投影阵列贴片技术（和其他方法）的皮肤靶向来改善重大疾病的免疫治疗做出重要贡献。