Center for Genetic Studies of Drug Abuse in Outbred Rats

项目总结/摘要（核心B：HS育种核心） N/NIH异质种群（HS）育种核心，在Leah Solberg Woods博士的指导下， 维克森林大学医学院（WFUSM）将为研究项目1-3提供HS大鼠。HS 动物从8个近交系大鼠品系远系繁殖并以最小化近亲繁殖的方式维持， 使数量性状基因座（QTL）的精细定位成为可能。索尔伯格·伍兹博士的实验室一直在维护 自2006年以来，她的实验室是北美唯一一个拥有这种独特资源的实验室。超过 在过去的四年里，作为P50卓越中心HS育种核心的一部分，HS大鼠群已经成为 国家资源。我们已经向P50内外的调查人员发送了老鼠。试点和可行性 通过P50资助的赠款已经为四个NIDA资助的使用HS大鼠的U 01赠款提供了资金。两 该核心的主要目标是：1）以最小化近亲繁殖的方式维持HS大鼠群体，2）运送 遗传多样的HS大鼠组的研究项目1-3。为了尽量减少近亲繁殖和控制遗传漂变， 并有足够的老鼠发送给调查人员在P50以及目前资助的U 01赠款， HS大鼠群将从64对繁殖鼠增加到92对繁殖鼠，并将使用随机繁殖来维持 战略，确保密切相关的动物不繁殖在一起。HS育种核心将运送HS大鼠 (male和女性）每年四到五次，研究项目1-3在四年的时间内，与研究 项目1在第5年接收有限数量的大鼠。通过提供一组遗传多样的HS大鼠， 研究项目1-3，本核心项目将为本P50中建议的研究提供基础，即 基因精细定位和识别与物质使用障碍相关的行为基因。

Project Summary/Abstract (Core B: HS Breeding Core) The N/NIH heterogeneous stock (HS) breeding core, under the direction of Dr. Leah Solberg Woods at the Wake Forest University School of Medicine (WFUSM), will provide HS rats to Research Project 1-3. HS animals are outbred from eight inbred rat strains and maintained in a manner that minimizes inbreeding, enabling the fine-mapping of quantitative trait loci (QTL). Dr. Solberg Woods’ laboratory has maintained the HS rat colony since 2006 and hers is the only laboratory in the North America to house this unique resource. Over the past four years as part of the P50 Center for Excellence HS Breeding Core, the HS rat colony has become a national resource. We have sent rats to investigators both inside and outside the P50. Pilot and Feasibility grants funded through the P50 have led to funding for four NIDA-funded U01 grants that use HS rats. The two main goals of this core will be to 1) maintain the HS rat colony in a way that minimizes inbreeding and 2) ship a genetically diverse set of HS rats to Research Projects 1-3. To minimize inbreeding and control genetic drift, as well as to have sufficient rat to send to investigators within the P50 as well as currently funded U01 grants, the HS rat colony will increase from 64 to 92 breeder pairs and will be maintained using a randomized breeding strategy, ensuring that closely related animals are not bred together. The HS Breeding Core will ship HS rats (male and female) four or five times per year to Research Projects 1-3 over a four year period, with Research Project 1 receiving a limited number of rats in year 5. By providing a genetically diverse set of HS rats to Research Projects 1-3, this Core will provide the back-bone for the studies proposed in this P50, namely the genetic fine-mapping and identification of genes involved in behaviors related to substance use disorders.