Center for Genetic Studies of Drug Abuse in Outbred Rats

项目摘要/摘要(核心B：HS育种核心) N/NIH异质种群(HS)育种核心，在Leah Solberg Wood博士的指导下 维克森林大学医学院(WFUSM)将为研究项目1-3提供HS大鼠。房协 动物是从8个近亲交配的老鼠品系中繁殖出来的，并以最大限度地减少近亲繁殖的方式进行维护， 实现数量性状基因座(QTL)的精细定位。索尔伯格·伍兹博士的实验室一直在维护HS 自2006年以来，她的实验室是北美唯一一个拥有这种独特资源的实验室。完毕 在过去的四年里，作为P50卓越HS繁育核心中心的一部分，HS鼠群已经成为 国家资源。我们已经向P50内外的调查人员发送了老鼠。试行和可行性 通过P50资助的赠款导致了四笔由NIDA资助的使用HS大鼠的U01赠款。两个人 这个核心的主要目标将是1)以最大限度地减少近亲繁殖的方式维持HS大鼠群体，2)运送 遗传多样性的HS大鼠集合到研究项目1-3。为了尽量减少近亲繁殖和控制遗传漂移，例如 并有足够的RAT发送给P50内的调查人员以及目前资助的U01赠款， HS大鼠群体将从增加到92对育种者，并将采用随机饲养的方式保持不变 战略，确保密切相关的动物不会一起繁殖。HS繁育核心将运送HS大鼠 (男性和女性)在四年内每年四到五次参加研究项目1-3，与研究 项目1在第5年接收有限数量的老鼠。通过提供一组遗传多样性的HS大鼠来 研究项目1-3，这一核心将为本P50中建议的研究提供基础，即 与药物使用障碍相关的行为相关基因的遗传精细定位和鉴定。

Project Summary/Abstract (Core B: HS Breeding Core) The N/NIH heterogeneous stock (HS) breeding core, under the direction of Dr. Leah Solberg Woods at the Wake Forest University School of Medicine (WFUSM), will provide HS rats to Research Project 1-3. HS animals are outbred from eight inbred rat strains and maintained in a manner that minimizes inbreeding, enabling the fine-mapping of quantitative trait loci (QTL). Dr. Solberg Woods’ laboratory has maintained the HS rat colony since 2006 and hers is the only laboratory in the North America to house this unique resource. Over the past four years as part of the P50 Center for Excellence HS Breeding Core, the HS rat colony has become a national resource. We have sent rats to investigators both inside and outside the P50. Pilot and Feasibility grants funded through the P50 have led to funding for four NIDA-funded U01 grants that use HS rats. The two main goals of this core will be to 1) maintain the HS rat colony in a way that minimizes inbreeding and 2) ship a genetically diverse set of HS rats to Research Projects 1-3. To minimize inbreeding and control genetic drift, as well as to have sufficient rat to send to investigators within the P50 as well as currently funded U01 grants, the HS rat colony will increase from 64 to 92 breeder pairs and will be maintained using a randomized breeding strategy, ensuring that closely related animals are not bred together. The HS Breeding Core will ship HS rats (male and female) four or five times per year to Research Projects 1-3 over a four year period, with Research Project 1 receiving a limited number of rats in year 5. By providing a genetically diverse set of HS rats to Research Projects 1-3, this Core will provide the back-bone for the studies proposed in this P50, namely the genetic fine-mapping and identification of genes involved in behaviors related to substance use disorders.