Biospecimen/Bioimaging Core

生物样本/生物成像核心

• 批准号: 10297621
• 负责人: Robert B West
• 金额: $ 31.89万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-15 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10297621
• 关键词: Antibodies; Atlases; Benign; Biopsy; Blood; Cancer Center Planning Grant; Cells; Communities; Consent; Cryopreservation; Data; Database Management Systems; Databases; Diagnosis; Education; Formalin; Freezing; Histology; Image; Immunohistochemistry; Infrastructure; International; Lasers; Link; Magnetic Resonance Imaging; Malignant neoplasm of prostate; Metals; Methods; Microdissection; Multiplexed Ion Beam Imaging; Paraffin Embedding; Pathology; Patients; Plasma; Process; Prostate; Prostatectomy; Quality Control; Radical Prostatectomy; Research; Research Personnel; Research Project Grants; Resolution; Resources; Scanning; Services; Stains; Standardization; Symptoms; Technology; Time; Tissue Embedding; Tissue Microarray; Tissue Procurements; Tissues; Training; Urology; bioimaging; demographics; digital; experience; laser capture microdissection; programs; prostate biopsy; training opportunity

ABSTRACT – BIOSPECIMEN / BIOIMAGING CORE The Biospecimen/Bioimaging Core leverages infrastructure of the Stanford Urology and Pathology departments, but augments services to benefit O’Brien Center investigators. Led by a qualified team, the centralized resource provides qualitative advantages and efficiencies of scale. The Core procures and provides Center investigators with needed biospecimens, including fresh, freshly-frozen, and formalin-fixed paraffin- embedded (FFPE) prostate issues. The Core also performs less visible but equally important activities, including patient consent, quality control/assurance, database management, clinicopathologic annotation, and regulatory compliance. In addition, the Core provides tissue characterization services, including histology, tissue microarrays, laser microdissection, and multiplex immunohistochemistry (IHC) using MIBI-TOF (Multiplexed Ion Beam Imaging by Time of Flight). MIBI-TOF is a powerful and robust technology that harnesses metal-tagged antibodies for the simultaneous quantification of 40 or more antibody targets, with superior spatial resolution and dynamic range. The Core also stores and provides portal access to images including prostate MRIs, whole-mount histology and IHC, and MIBI-TOF data, as well as an integrated multi- scale prostate Atlas spanning MRI to histology, cells and molecules. The Biospecimen/Bioimaging Core provides essential tissue procurement, characterization and imaging services to support the three Center Projects, the Education Enrichment and Opportunity Pool programs, as well as outside O’Brien Centers, P20 grant programs, and the broader benign urology community, .

摘要-生物标本/生物成像核心 生物标本/生物成像核心利用了斯坦福大学泌尿学和病理学的基础设施 部门，但增加服务，使奥布莱恩中心的调查人员受益。在一个合格的团队的领导下， 集中的资源提供质量优势和规模效率。核心采购和提供 中心研究者需要生物标本，包括新鲜标本、新鲜冷冻标本和福尔马林固定石蜡标本- 嵌入式（FFPE）前列腺问题。核心还执行不太明显但同样重要的活动， 包括患者知情同意、质量控制/保证、数据库管理、临床病理学注释，以及 合规性。此外，核心提供组织表征服务，包括组织学， 组织微阵列、激光显微切割和使用MIBI-TOF的多重免疫组织化学（IHC） （通过飞行时间的多路复用离子束成像）。MIBI-TOF是一种强大而稳健的技术， 利用金属标记的抗体同时定量40个或更多的抗体靶标， 上级空间分辨率和动态范围。Core还存储并提供对图像的门户访问 包括前列腺MRI，整体组织学和IHC，MIBI-TOF数据，以及集成的多- 将前列腺图谱从MRI扩展到组织学、细胞和分子。生物标本/生物成像核心 提供基本的组织采购、表征和成像服务，以支持三个中心 项目，教育丰富和机会池计划，以及外部奥布莱恩中心，P20 资助项目以及更广泛的良性泌尿外科界。