Multi-modal cell type atlases of somatosensory spinal cord neurons

体感脊髓神经元多模态细胞类型图谱

基本信息

  • 批准号:
    10743857
  • 负责人:
  • 金额:
    $ 40.07万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2022
  • 资助国家:
    美国
  • 起止时间:
    2022-07-15 至 2024-06-30
  • 项目状态:
    已结题

项目摘要

PROJECT SUMMARY/ABSTRACT The major objective of this proposal is to classify somatosensory neurons in a more integrated manner by capturing the transcriptomic, electrophysiological, and morphological properties of individual neurons in the spinal dorsal horn (SDH) using the Patch-seq technique. The SDH is critical for processing distinct modalities of sensation, such as touch, temperature, chemical, itch, and pain. Neurons in SDH are heterogenous and composed of a vast majority of excitatory and inhibitory interneurons that exhibit a wide range of morphological, physiological, and transcriptomic properties. Cell-type classification in the SDH provides a logistical and conceptual framework for understanding how cells and circuits govern somatic sensation. Attempts to classify neurons include morphological and/or electrophysiological properties, and more recently by transcriptomic features. However, linking transcriptomically defined cell types in SDH to their electrophysiological/morphological phenotypes remains a major challenge. The single-cell RNA-sequencing (scRNA-seq) coupled with patch clamp recording (Patch-seq) is a powerful approach that enables one to directly relate the transcriptomic features of a given neuron to the phenotypes of the same neuron, e.g., the neuron’s precise location, morphology, electrophysiological properties, and the functions. In the preliminary study, we have successfully used this technique to sample SDH neurons and acquired the neuron’s intrinsic firing properties and reconstructed morphology from patched neurons. In this proposal, we will use the Patch-seq technique to classify somatosensory neuron types in a more integrated manner by capturing the transcriptomic, electrophysiological, and morphological properties of individual neurons in mouse SDH. Our proposed study will bridge the missing link between transcriptomically defined cell types and morphological/electrophysiological properties of somatosensory neurons in the SDH, which provide a critical stepping-stone for illustrating how the SDH codes and relays distinct sensory modalities and how disruption of sensory processing in the SDH causes chronic pain and itch.
项目总结/文摘

项目成果

期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Characterization of synaptic structural plasticity in mouse spinal dorsal horn neurons.
  • DOI:
    10.1016/j.xpro.2023.102752
  • 发表时间:
    2023-12-15
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Ru, Qin;Magnusson, Jennifer;Li, Lingyong
  • 通讯作者:
    Li, Lingyong
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Lingyong Li其他文献

Lingyong Li的其他文献

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{{ truncateString('Lingyong Li', 18)}}的其他基金

Targeting Tiam1-mediated synaptic plasticity for the relief of opioid tolerance
靶向 Tiam1 介导的突触可塑性以缓解阿片类药物耐受
  • 批准号:
    10800301
  • 财政年份:
    2023
  • 资助金额:
    $ 40.07万
  • 项目类别:
The conserved mechanisms underlying different types of chronic pain
不同类型慢性疼痛的保守机制
  • 批准号:
    10677714
  • 财政年份:
    2022
  • 资助金额:
    $ 40.07万
  • 项目类别:
Targeting Tiam1-mediated synaptic plasticity for the relief of opioid tolerance
靶向 Tiam1 介导的突触可塑性以缓解阿片类药物耐受
  • 批准号:
    10512217
  • 财政年份:
    2022
  • 资助金额:
    $ 40.07万
  • 项目类别:
Multi-modal cell type atlases of somatosensory spinal cord neurons
体感脊髓神经元多模态细胞类型图谱
  • 批准号:
    10508739
  • 财政年份:
    2022
  • 资助金额:
    $ 40.07万
  • 项目类别:
Alternative polyadenylation(APA) mechanisms of comorbid mood disorders in chronic pain
慢性疼痛共病情绪障碍的替代多聚腺苷酸化(APA)机制
  • 批准号:
    10572902
  • 财政年份:
    2022
  • 资助金额:
    $ 40.07万
  • 项目类别:
The conserved mechanisms underlying different types of chronic pain
不同类型慢性疼痛的保守机制
  • 批准号:
    10747177
  • 财政年份:
    2022
  • 资助金额:
    $ 40.07万
  • 项目类别:

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