Automated Liquid Handler

7.项目摘要/摘要 该提案要求提供资金购买美国制造的哈密尔顿NGS STAR液体处理平台， 包括安装、培训和内华达州里诺市哈密尔顿公司提供的12个月保修。制度支持 帮助支付采购费用，并支付设施费用、人员工资和服务合同 仪器的使用寿命。该系统将与我们现有的下一代测序一起安装 仪器包括Illumina NovaSeq 6000、Illumina NextSeq 500、两个Illumina MiSeq平台、 国家癌症研究所(NCI)的10x基因组铬控制器和GenapSys测序平台- 指定Sidney Kimmel癌症中心(SKCC)收费服务MetaOmics共享资源核心设施，地址为 位于费城中部的托马斯·杰斐逊大学(TJU)。哈姆利顿NGS明星将使下一代 TJU的测序需求，包括SKCC及其区域财团合作伙伴Drexel大学。建基 在2006年，MetaOmics共享资源通过促进基因组学研究 数百个利用各种实验系统的研究项目。MetaOmics共享资源 最初提供的高通量测序使用两项生命技术/应用生物系统5500xl基因 分析仪和两台离子激流PGM仪器，由Illumina系统取代，包括 NovaSeq 6000，提供所有商业测序仪器中最高的吞吐量。这个 NovaSeq 6000是在2021年通过S10拨款获得的，它将从自动化样本处理中受益匪浅 以实现最佳运行。这些平台的推出刺激了测序需求的增长，超过了 我们的人工操作能力。收购汉密尔顿NGS STAR平台将确保实验室 在这个机构的支持下，特别是这里描述的NIH资助的调查人员以及年轻的 依赖MetaOmics共享资源的调查人员将继续他们的第一份NIH拨款提案 及时获得高性价比的服务。汉密尔顿NGS明星与Illumina合作 测序系统提供更高的精密度、重复性、更大的样本容量、更快的周转速度和 更具成本效益的测序。更高的效率和成本节约将支持和加快研究 遗传性疾病，复杂的特征，以及肿瘤发生、转移和治疗反应的机制。 这项建议的优点包括：来自NCI指定的SKCC和TJU管理部门的支持；领导力 私人投资和关键人员的经验和生物技术专业知识；TJU的大型基础设施 学术、临床和创新支柱；具有创新研究项目的高效教师队伍。采办 该设备的研发将对台湾大学的生物医学研究产生长期影响。

7. PROJECT SUMMARY/ABSTRACT This proposal requests funds for purchase of a US-manufactured Hamilton NGS STAR liquid handling platform, including installation, training, and a 12-month warranty from Hamilton Company, Reno, NV. Institutional support is to help defray purchasing cost and to cover facility costs, personnel salaries, and the service contract for the lifetime of the instrument. The system will be housed with our existing next generation sequencing instrumentation including the Illumina NovaSeq 6000, Illumina NextSeq 500, two Illumina MiSeq platforms, 10xGenomics Chromium Controller, and GenapSys sequencing platforms in the National Cancer Institute (NCI)- designated Sidney Kimmel Cancer Center (SKCC) fee-for-service MetaOmics Shared Resource core facility at Thomas Jefferson University (TJU) in central Philadelphia. The Hamliton NGS STAR will enable next-generation sequencing demands at TJU, including the SKCC and its regional consortium partner Drexel University. Founded in 2006, the MetaOmics Shared Resource has been instrumental in promoting genomics research by facilitating hundreds of research projects utilizing a wide variety of experimental systems. The MetaOmics Shared Resource initially offered high-throughput sequencing using two Life Technologies/Applied Biosystems 5500xl Genetic Analyzers and two Ion Torrent PGM instruments, which were replaced by Illumina systems including the NovaSeq 6000, offering the highest throughput of any commercially available sequencing instrument. The NovaSeq 6000, acquired through an S10 grant in 2021, would greatly benefit from automated sample handling for optimal operation. Availability of these platforms has spurred an increase in sequencing demand that exceeds our manual handling capabilities. Acquisition of the Hamilton NGS STAR platform will ensure that laboratories supported by this facility, especially the NIH-funded investigators described herein, as well as young investigators who rely on MetaOmics Shared Resource resources for their first NIH grant proposals, will continue to receive timely and cost-effective service. The Hamilton NGS STAR works in conjunction with Illumina Sequencing Systems to offer higher precision, reproducibility, increased sample capacity, faster turnaround and more cost-effective sequencing. Increased efficiency and cost savings will support and expedite research in genetic diseases, complex traits, and mechanisms of tumorigenesis, metastasis, and therapeutic response. Strengths of this proposal include: support from NCI-designated SKCC and TJU administration; leadership experience and biotechnology expertise of the PI and key personnel; the large-scale infrastructure of the TJU academic, clinical, and innovation pillars; highly productive faculty with innovative research projects. Acquisition of this equipment will have long-lasting impact on biomedical research at TJU.