Cancer Genomics

癌症基因组学共享资源 2006年7月，为了更好地满足基梅尔癌症中心调查人员的需求，前核酸和 定制微阵列设备被重组为癌症基因组学共享资源。中心 聘请了一名新的董事、经理和高级技术员。这一新资源提供了最先进的分子 促进基础、转化性和临床研究项目的分析和专业知识。通过担任 通过各种平台提供全基因组和针对基因的服务的集中资源，它提供了 服务范围广，质量控制高，调查人员成本低。此共享资源是 为应对基因阵列的技术进步和高通量需求而建立 表达分析和全基因组分析以研究发病机制、治疗方法、遗传 癌症研究中的敏感性和基因发现。对于基因表达，该设施提供内部 用于microRNA分析的打印幻灯片以及使用Affymetrix基因芯片平台的商业阵列。 基于微阵列的mRNA表达谱定义的差异表达转录本得到确认 实时荧光定量RT-PCR检测。由于信息学是最重要的，癌症基因组学分享了 资源无缝链接到新的生物统计共享资源，并与 蛋白质设施。此外，它还为DNA序列分析提供服务，吞吐量增加 通过开发基于网络的程序来订购寡核苷酸来提交DNA测序， 检索排序结果并计算共享资源使用情况。为基于Taqman的客户提供服务 SNP分型、使用单碱基延伸(SBE)和焦磷酸测序的多重SNP基因分型 ABI 3730、Beckman Coulter GenomeLab SNPstream和焦磷酸测序PSQ HS 96A的化学 平台和用于全基因组关联的Affymetrix SNP映射基因芯片 研究、连锁分析和拷贝数变异。它支持基于DNA序列和基因分型 项目范围从单一SNPs到定制的多重基因再到全基因组面板。共享资源 随着阵列技术领域的变化而灵活适应。此共享资源将扩展其 服务包括染色质占有率和与分化、描绘相关的表观遗传学研究 了解正常状态和疾病状态之间的差异，并监测对药物的反应。常规微阵列 新技术研讨会由Affymetrix，Inc.(圣诞老人)等大公司每两个月举办一次 加利福尼亚州克拉拉)、安捷伦技术公司(加利福尼亚州圣克拉拉)、Luminex Corp.(德克萨斯州奥斯汀)、Beckman Coulter、 公司(加利福尼亚州富勒顿)和应用生物系统公司(加利福尼亚州福斯特城)。此外，来自用户的演示文稿 为所有中心成员和TJU社区每月安排一次。

CANCER GENOMICS SHARED RESOURCE In July 2006, to better meet the needs of Kimmel Cancer Center investigators, the former Nucleic Acid and Custom Microarray Facilities were restructured into the Cancer Genomics Shared Resource. The center hired a new director, manager and senior technician. This new resource provides state-of-the-art molecular assays and expertise to facilitate projects in basic, translational and clinical research. By serving as a centralized resource with a variety of platforms for genome-wide and gene-targeted services, it offers a wide spectrum of services, high quality control and low costs to investigators. This shared resource was established in response to technological advances in gene arrays and demand for high-throughput expression analysis and genome-wide analyses to investigate pathogenesis, therapeutics, genetic susceptibility and gene discovery in cancer research. For gene expression, this facility offers in-house printed slides for microRNA analysis as well as commercial arrays using the Affymetrix GeneChip platform. Differentially expressed transcripts defined by microarray-based mRNA expression profiling are confirmed by real-time RT-PCR. Since informatics is of paramount importance, the Cancer Genomics Shared Resource operates seamlessly linked to the new Biostatistic Shared Resource and has close links to the protein facility. In addition, it provides service for DNA sequence analysis, with increased throughput of DNA sequencing submission through development of a web-based program for ordering oligonucleotides, retrieving sequencing results and accounting for Shared Resource usage. Service is provided for Taqmanbased SNP typing, multiplex SNP genotyping using single-base extension (SBE) and pyrosequencing chemistry on ABI 3730, Beckman Coulter GenomeLab SNPstream and Pyrosequencing PSQ HS 96A platforms, respectively, and Affymetrix SNP Mapping GeneChips for use in genome-wide association studies, linkage analysis and copy number variation. It supports DNA sequence-based and genotyping projects ranging from single SNPs through custom multiplex to whole-genome panels. The shared resource has the flexibility to adapt, as the field of array technology changes. This shared resource will expand its services to include chromatin occupancy and epigenetic studies associated with differentiation, delineation of differences between normal and diseased states, and monitoring response to drugs. Regular microarray and novel technology seminars are provided bimonthly by major companies such as Affymetrix, Inc. (Santa Clara, CA), Agilent Technologies, Inc. (Santa Clara, CA), Luminex Corp. (Austin, TX), Beckman Coulter, Inc. (Fullerton, CA) and Applied Biosystems (Foster City, CA). In addition, presentations from users are arranged on a monthly basis for all center members and the TJU community.