Cancer Genomics

癌症基因组学共享资源 2006年7月，为了更好地满足Kimmel癌症中心研究人员的需求，前核酸和 定制微阵列设施被重组为癌症基因组学共享资源。中心 聘请了新的主任，经理和高级技术员。这种新的资源提供了最先进的分子 分析和专业知识，以促进基础，转化和临床研究项目。通过充当 集中的资源与各种平台的基因组范围和基因靶向服务，它提供了一个 服务范围广，质量控制高，调查费用低。这个共享资源是 为了应对基因阵列技术的进步和高通量的需求， 表达分析和全基因组分析，以研究发病机制，治疗，遗传 癌症研究中的易感性和基因发现。对于基因表达，该设施提供内部 用于microRNA分析的打印载玻片以及使用Affyssin GeneChip平台的商业阵列。 通过基于微阵列的mRNA表达谱确定的差异表达转录本得到证实 实时RT-PCR。由于信息学是至关重要的，癌症基因组学共享 资源无缝链接到新的生物统计共享资源，并与 蛋白质设施此外，它还提供DNA序列分析服务， 通过开发用于订购寡核苷酸的基于网络的程序提交DNA测序， 检索排序结果并说明共享资源的使用情况。服务基于Taqman SNP分型，使用单碱基延伸（SBE）和焦磷酸测序的多重SNP基因分型 ABI 3730、Beckman Coulter GenomeLab SNPstream和焦磷酸测序PSQ HS 96A上的化学 平台，分别和用于全基因组关联的AffyssSNP作图基因芯片 研究、连锁分析和拷贝数变异。它支持基于DNA序列和基因分型 项目范围从单一SNP到定制的多路复用到全基因组面板。共享资源 具有适应阵列技术领域变化的灵活性。这种共享资源将扩大其 服务包括染色质占有率和与分化、描绘 正常和疾病状态之间的差异，以及监测对药物的反应。规则微阵列 和新技术研讨会由大公司如Affybet，Inc.（圣诞老人 Clara，CA）、Agilent Technologies，Inc.（Santa Clara，CA），Luminex Corp.（Austin，TX），Beckman Coulter， Inc.（Fullerton，CA）和Applied Biosystems（Foster City，CA）。此外，用户的演示文稿 每月为所有中心成员和TJU社区安排。

CANCER GENOMICS SHARED RESOURCE In July 2006, to better meet the needs of Kimmel Cancer Center investigators, the former Nucleic Acid and Custom Microarray Facilities were restructured into the Cancer Genomics Shared Resource. The center hired a new director, manager and senior technician. This new resource provides state-of-the-art molecular assays and expertise to facilitate projects in basic, translational and clinical research. By serving as a centralized resource with a variety of platforms for genome-wide and gene-targeted services, it offers a wide spectrum of services, high quality control and low costs to investigators. This shared resource was established in response to technological advances in gene arrays and demand for high-throughput expression analysis and genome-wide analyses to investigate pathogenesis, therapeutics, genetic susceptibility and gene discovery in cancer research. For gene expression, this facility offers in-house printed slides for microRNA analysis as well as commercial arrays using the Affymetrix GeneChip platform. Differentially expressed transcripts defined by microarray-based mRNA expression profiling are confirmed by real-time RT-PCR. Since informatics is of paramount importance, the Cancer Genomics Shared Resource operates seamlessly linked to the new Biostatistic Shared Resource and has close links to the protein facility. In addition, it provides service for DNA sequence analysis, with increased throughput of DNA sequencing submission through development of a web-based program for ordering oligonucleotides, retrieving sequencing results and accounting for Shared Resource usage. Service is provided for Taqmanbased SNP typing, multiplex SNP genotyping using single-base extension (SBE) and pyrosequencing chemistry on ABI 3730, Beckman Coulter GenomeLab SNPstream and Pyrosequencing PSQ HS 96A platforms, respectively, and Affymetrix SNP Mapping GeneChips for use in genome-wide association studies, linkage analysis and copy number variation. It supports DNA sequence-based and genotyping projects ranging from single SNPs through custom multiplex to whole-genome panels. The shared resource has the flexibility to adapt, as the field of array technology changes. This shared resource will expand its services to include chromatin occupancy and epigenetic studies associated with differentiation, delineation of differences between normal and diseased states, and monitoring response to drugs. Regular microarray and novel technology seminars are provided bimonthly by major companies such as Affymetrix, Inc. (Santa Clara, CA), Agilent Technologies, Inc. (Santa Clara, CA), Luminex Corp. (Austin, TX), Beckman Coulter, Inc. (Fullerton, CA) and Applied Biosystems (Foster City, CA). In addition, presentations from users are arranged on a monthly basis for all center members and the TJU community.