Onco-Genomics Shared Resources

肿瘤基因组学共享资源

• 批准号: 10443638
• 负责人: SION LLEWELYN WILLIAMS
• 金额: $ 18.99万
• 依托单位: UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-07-10 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10443638
• 关键词: ATAC-seq; Applications Grants; Award; Bioinformatics Shared Resource; Biological Assay; Biometry; Cancer Center Support Grant; Cancer Control; Cells; ChIP-seq; Charge; Chemistry; Chromium; Comprehensive Cancer Center; Consultations; DNA sequencing; Data Analyses; Education; Epigenetic Process; Experimental Designs; Feedback; Flow Cytometry Shared Resource; Funding; Gene Expression; Genomics; Grant; Hour; Intervention; Laboratories; Libraries; Liquid substance; Malignant Neoplasms; Molecular; Nucleic Acids; Peer Review; Preparation; Quality Control; Research; Research Personnel; Research Priority; Resource Sharing; Resources; Running; Sampling; Science; Services; Societies; Specialized Center; Surveys; System; Technology; Time; Training; Translational Research; Tumor Biology; Validation; Work; cancer genomics; cost effective; data quality; design; epigenomics; experimental study; functional genomics; instrumentation; leukemia/lymphoma; member; nano-string; next generation sequencing; peer support; programs; satisfaction; service providers; transcriptome sequencing; transcriptomics

PROJECT SUMMARY: ONCO-GENOMICS SHARED RESOURCE The Onco-Genomics Shared Resource (OGSR) provides members of the Sylvester Comprehensive Cancer Center (Sylvester) access to technology platforms and assays for studying the genomics and epigenetics of cancer that are far too specialized for any one laboratory to support on its own. The OGSR employs four full- time technicians and one part-time technician who are cross-trained on workflows and quality control assessments. The facility is charged with providing rapid turnaround times, a service menu tailored to Sylvester investigator needs, and cost-effective use of resources. Next generation sequencing (NGS) capacity is maximized with automated library preparation and the ability to simultaneously run two different sequencing chemistries. State-of-the-art instrumentation includes two Illumina NextSeq 500 benchtop sequencers, a Perkin Elmer Zephyr G3 NGS liquid handler, a 10x Genomics Chromium single cell controller, and a nanoString MAX system. NGS services support RNA-seq of all types and DNA-seq for epigenetics workflows, such as ChIP-seq and ATAC-seq. Additionally, staff work closely with investigators to develop workflows for challenging library types and single cell experiments. The OGSR also offers basic services for investigators who lack laboratory resources, such as sample extraction and quality control, to assist in furthering translational research. In coordination with the Biostatistics and Bioinformatics Shared Resource (BBSR) and the Flow Cytometry Shared Resource (FCSR), the OGSR provides expert support for experimental design and data interpretation to optimize grant applications and drive post-award science. In 2017, the OGSR provided services to 27 Sylvester investigators (89% of whom were supported by peer-reviewed funding). Sylvester members comprise 69% of total OGSR users.

项目摘要：肿瘤基因组学共享资源 肿瘤基因组学共享资源(OGSR)提供西尔维斯特综合癌症组织的成员 中心(西尔维斯特)获得技术平台和分析，以研究基因组学和表观遗传学 癌症太过专门化，任何一个实验室都无法单独支持。OGSR雇用了四名全职- 接受过工作流程和质量控制交叉培训的兼职技术员和一名兼职技术员 评估。该设施负责提供快速周转时间，服务菜单量身定做 西尔维斯特调查人员的需求，以及对资源的成本效益使用。下一代排序(NGS)容量 通过自动化文库准备和同时运行两种不同测序的能力最大化 化学药学。最先进的仪器包括两个Illumina NextSeq 500台式测序仪，一个Perkin 埃尔默·泽菲尔G3 NGS液体处理器、10倍基因组铬单电池控制器和NanoStringMax 系统。NGS服务支持所有类型的rna-seq和表观遗传学工作流的dna-seq，例如Chip-seq。 和ATAC-seq.此外，工作人员与调查人员密切合作，制定挑战图书馆的工作流程 类型和单细胞实验。OGSR还为缺乏实验室的调查人员提供基本服务 提供样本提取和质量控制等资源，以协助进一步开展翻译研究。在……里面 与生物统计学和生物信息学共享资源(BBSR)和流式细胞术的协调 共享资源(FCSR)，OGSR为实验设计和数据解释提供专家支持 优化拨款申请，推动获奖后的科学研究。2017年，OGSR向27家企业提供服务 西尔维斯特研究人员(其中89%得到同行审查资金的支持)。西尔维斯特成员包括 占OGSR用户总数的69%。