Onco-Genomics Shared Resources

肿瘤基因组学共享资源

• 批准号: 9789585
• 负责人: SION LLEWELYN WILLIAMS
• 金额: $ 19.42万
• 依托单位: UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-07-10 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9789585
• 关键词: ATAC-seq; Applications Grants; Award; Bioinformatics Shared Resource; Biological Assay; Biometry; Cancer Center Support Grant; Cancer Control; Cells; ChIP-seq; Charge; Chemistry; Chromium; Comprehensive Cancer Center; Consultations; DNA sequencing; Data Analyses; Data Quality; Education; Epigenetic Process; Experimental Designs; Feedback; Flow Cytometry Shared Resource; Funding; Gene Expression; Genomics; Grant; Hour; Intervention; Laboratories; Libraries; Liquid substance; Malignant Neoplasms; Molecular; Nucleic Acids; Peer Review; Preparation; Quality Control; Research; Research Personnel; Research Priority; Resource Sharing; Resources; Running; Sampling; Science; Services; Societies; Specialized Center; Surveys; System; Technology; Time; Training; Translational Research; Tumor Biology; Validation; Work; cancer genomics; cost effective; design; epigenomics; experimental study; functional genomics; instrumentation; leukemia/lymphoma; member; nano-string; next generation sequencing; peer support; programs; satisfaction; service providers; transcriptome sequencing; transcriptomics

PROJECT SUMMARY: ONCO-GENOMICS SHARED RESOURCE The Onco-Genomics Shared Resource (OGSR) provides members of the Sylvester Comprehensive Cancer Center (Sylvester) access to technology platforms and assays for studying the genomics and epigenetics of cancer that are far too specialized for any one laboratory to support on its own. The OGSR employs four full- time technicians and one part-time technician who are cross-trained on workflows and quality control assessments. The facility is charged with providing rapid turnaround times, a service menu tailored to Sylvester investigator needs, and cost-effective use of resources. Next generation sequencing (NGS) capacity is maximized with automated library preparation and the ability to simultaneously run two different sequencing chemistries. State-of-the-art instrumentation includes two Illumina NextSeq 500 benchtop sequencers, a Perkin Elmer Zephyr G3 NGS liquid handler, a 10x Genomics Chromium single cell controller, and a nanoString MAX system. NGS services support RNA-seq of all types and DNA-seq for epigenetics workflows, such as ChIP-seq and ATAC-seq. Additionally, staff work closely with investigators to develop workflows for challenging library types and single cell experiments. The OGSR also offers basic services for investigators who lack laboratory resources, such as sample extraction and quality control, to assist in furthering translational research. In coordination with the Biostatistics and Bioinformatics Shared Resource (BBSR) and the Flow Cytometry Shared Resource (FCSR), the OGSR provides expert support for experimental design and data interpretation to optimize grant applications and drive post-award science. In 2017, the OGSR provided services to 27 Sylvester investigators (89% of whom were supported by peer-reviewed funding). Sylvester members comprise 69% of total OGSR users.

项目概要：肿瘤基因组学共享资源 肿瘤基因组学共享资源（OGSR）为西尔维斯特综合癌症研究所的成员提供了 中心（西尔维斯特）获得技术平台和分析研究的基因组学和表观遗传学 这些癌症过于专业化，任何一个实验室都无法单独支持。OGSR雇用了四名全职员工- 1名全职技术人员和1名兼职技术人员，他们接受过工作流程和质量控制方面的交叉培训 评估。该设施负责提供快速的周转时间， 西尔维斯特调查员的需求，并具有成本效益的资源使用。下一代测序（NGS）能力 通过自动化文库制备和同时运行两种不同测序的能力， 化学最先进的仪器包括两台Illumina NextSeq 500台式测序仪，一台Perkin测序仪， Elmer Zephyr G3 NGS液体处理器、10 x Genomics Chromium单细胞控制器和nanoString MAX 系统NGS服务支持所有类型的RNA-seq和用于表观遗传学工作流程的DNA-seq，例如ChIP-seq 和ATAC-seq.此外，工作人员与调查人员密切合作，制定工作流程， 类型和单细胞实验。OGSR还为缺乏实验室的研究人员提供基本服务 资源，如样品提取和质量控制，以协助进一步的转化研究。在 与生物统计学和生物信息学共享资源（BBSR）和流式细胞术协调 OGSR为实验设计和数据解释提供专家支持 优化拨款申请并推动奖后科学。2017年，OGSR为27个 西尔维斯特研究人员（其中89%得到同行评审资金的支持）。西尔维斯特的成员包括 69%的OGSR用户。