Molecular Diagnostic Tools for Patient Oriented Field Studies in Infectious Diseases

用于以患者为导向的传染病现场研究的分子诊断工具

• 批准号: 10443710
• 负责人: ERIC R HOUPT
• 金额: $ 15.76万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10443710
• 关键词: Antimicrobial Resistance; Area; Award; Childhood; Clinical Trials; Clinical Trials Design; Communicable Diseases; Communities; Cryptosporidium; Data; Data Analyses; Development; Diagnosis; Diagnostic; Diagnostics Research; Diarrhea; Drug resistance; Escherichia coli Vaccines; Etiology; Faculty; Fellowship; Funding; Grant; Infection; Infectious Diseases Research; International; K-Series Research Career Programs; Malaria; Mentors; Midcareer Investigator Award in Patient-Oriented Research; Molecular Diagnosis; Multidrug-Resistant Tuberculosis; NIH Program Announcements; Population; Research; Research Personnel; Schools; Series; Serotyping; Site; Streptococcus pneumoniae; Therapeutic; Therapeutic Studies; Time; Tuberculosis; United States National Institutes of Health; Universities; Validation; Virginia; Work; base; diagnostic tool; enteric infection; enterotoxigenic Escherichia coli; experience; field study; medical schools; meetings; molecular diagnostics; patient oriented; patient oriented research; primary outcome; programs; secondary outcome; skills; symposium

I am a Clinician Investigator in the Division of Infectious Diseases at University of Virginia and am submitting this one-time K24 renewal application to further extend my molecular diagnostics research into patient oriented research (POR) and to provide protected time to mentor both my direct and institutional trainees. In the 15 years since completion of ID fellowship I have developed a research program in several domains of tropical infectious diseases, now largely focused on the development of molecular diagnostic tools for research. Aim 1: In the first term of this grant I established the University of Virginia School of Medicine K-award mentoring program, which entails a series of Symposia and meetings geared towards both those applying for and those who have already received NIH K or similar awards. This K24 grant will allow me to continue and extend this important program further. This grant will also allow protected time to directly mentor my trainees in POR, which currently consists of 3 K23 recipients and 1 Infectious Disease fellow, working in areas spanning MDR TB, pediatric TB, childhood diarrhea, and antimicrobial resistance, each with a diagnostic emphasis. Aim 2: I will also extend my personal research program into POR. Currently funded work includes the use of molecular diagnostics for both case- and population-level etiologic diagnosis of diarrhea and molecular diagnosis of MDR Tuberculosis. The most mature work involves diarrheal etiology, and our latest data strongly supports entering into clinical trials for Enterotoxigenic E. coli vaccines and new Cryptosporidium therapeutics. This K24 will allow me protected time to insert these molecular diagnostics into these trials as primary or secondary outcomes and gain needed skills in clinical trial design and data analysis. Finally, consonant with the K24 mechanism, I will expand my research new areas such as molecular diagnostics for antimicrobial resistance.

我是弗吉尼亚大学传染病系的临床研究员，现提交 这一次K24续订申请进一步将我的分子诊断学研究扩展到以患者为中心 研究(POR)，并提供有保障的时间来指导我的直接和机构实习生。在15个国家中 在完成ID奖学金后的几年里，我在热带的几个领域制定了一个研究计划 传染病，现在主要集中在开发分子诊断工具进行研究。目标1： 在这笔赠款的第一个任期内，我建立了弗吉尼亚大学医学院K奖导师 计划，其中包括一系列针对申请者和申请者的研讨会和会议 已经获得NIH K或类似奖项的人。这笔K24拨款将允许我继续并延长这一计划 更重要的计划。这笔拨款还将允许受保护的时间直接指导我在POR中的实习生， 目前由3名K23获奖者和1名传染病研究员组成，在跨越MDR的地区工作 结核病、儿童结核病、儿童腹泻和抗菌素耐药性，每一项都有诊断重点。目标2：我 还将把我的个人研究项目扩展到POR。目前资助的工作包括使用分子 腹泻的病例和人群病原学诊断及耐多药的分子诊断 肺结核。最成熟的工作涉及腹泻病因学，我们的最新数据有力地支持进入 进入产肠毒素大肠杆菌疫苗和隐孢子虫新疗法的临床试验。这款K24将 允许我有保护的时间将这些分子诊断作为主要或次要试验插入到这些试验中 临床试验设计和数据分析方面的成果和所需技能。最后，与K24的辅音 机制方面，我将拓展我的研究新领域，如抗菌素耐药性的分子诊断。

项目成果

Etiology of severe invasive infections in young infants in rural settings in sub-Saharan Africa.

• DOI: 10.1371/journal.pone.0264322
• 发表时间: 2022
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Mduma E;Halidou T;Kaboré B;Walongo T;Lompo P;Museveni J;Gidabayda J;Gratz J;Guga G;Kimathi C;Liu J;Mdoe P;Moshiro R;Petzold M;Singlovic J;Guillerm M;Gomes MF;Houpt ER;Halleux CM
• 通讯作者: Halleux CM

Evaluation of Molecular Serotyping Assays for Shigella flexneri Directly on Stool Samples.

直接在粪便样品上的志贺氏菌柔韧性分子血清分型测定评估。

• DOI: 10.1128/jcm.02455-20
• 发表时间: 2021-01-21
• 期刊: Journal of clinical microbiology
• 影响因子: 9.4
• 作者: Liu J;Pholwat S;Zhang J;Taniuchi M;Haque R;Alam M;Ochieng JB;Jones JA;Platts-Mills JA;Tennant SM;Houpt E
• 通讯作者: Houpt E

Clofazimine for Treatment of Cryptosporidiosis in Human Immunodeficiency Virus Infected Adults: An Experimental Medicine, Randomized, Double-blind, Placebo-controlled Phase 2a Trial.

• DOI: 10.1093/cid/ciaa421
• 发表时间: 2021-07-15
• 期刊: Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
• 作者: Iroh Tam P;Arnold SLM;Barrett LK;Chen CR;Conrad TM;Douglas E;Gordon MA;Hebert D;Henrion M;Hermann D;Hollingsworth B;Houpt E;Jere KC;Lindblad R;Love MS;Makhaza L;McNamara CW;Nedi W;Nyirenda J;Operario DJ;Phulusa J;Quinnan GV;Sawyer LA;Thole H;Toto N;Winter A;Van Voorhis WC
• 通讯作者: Van Voorhis WC

Detection of Pathogens of Acute Febrile Illness Using Polymerase Chain Reaction from Dried Blood Spots.

• DOI: 10.4269/ajtmh.21-0814
• 发表时间: 2021-11-08
• 期刊: The American journal of tropical medicine and hygiene
• 作者: Grundy B;Panzner U;Liu J;Jeon HJ;Im J;von Kalckreuth V;Konings F;Pak GD;Cruz Espinoza LM;Bassiahi AS;Gasmelseed N;Rakotozandrindrainy R;Stroup S;Houpt ER;Marks F
• 通讯作者: Marks F

Pathogen Detection Using Metagenomic Next-Generation Sequencing of Plasma Samples from Patients with Sepsis in Uganda.

• DOI: 10.1128/spectrum.04312-22
• 发表时间: 2023-02-14
• 期刊: Microbiology spectrum
• 影响因子: 3.7