The Nanopore-based Ion Selective Electrode Vial

基于纳米孔的离子选择电极瓶

• 批准号: 10325420
• 负责人: Sean German
• 金额: $ 35万
• 依托单位: ELECTRONIC BIOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-20 至 2023-09-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10325420
• 关键词: Anion Gap; Biological; Biological Assay; Biological Markers; Biological Sciences; Blood; Blood Tests; Blood specimen; Brain; Buffers; Calcium; Calibration; Carbon Dioxide; Charge; Chelating Agents; Chemicals; Chemistry; Chronic Kidney Failure; Collection; Complete Blood Count; Dependence; Detection; Development; Devices; Diagnosis; Electrochemistry; Electrodes; Electrolytes; Electronics; Elements; Emergency Medicine; Ensure; Enzyme Electrodes; Enzymes; Equation; Equilibrium; Evaluation; Filtration; Fingers; Fluid Balance; Food Energy; Foundations; German population; Glass; Gold; Health; Home; Individual; Ion Exchange; Ion-Selective Electrodes; Ions; Iron; Laboratories; Maintenance; Manuals; Measurable; Measurement; Measures; Medical; Membrane; Metabolic; Methodology; Molecular; Monitor; Muscle Contraction; Nanotechnology; Nutrient; Patient Care; Patient-Focused Outcomes; Patients; Performance; Pharmaceutical Preparations; Phase; Physicians; Physiological; Plasma; Polymers; Potassium; Potassium Chloride; Process; Reader; Sampling; Side; Signal Transduction; Small Business Technology Transfer Research; Sodium; Specialist; Structure; System; Systems Development; Systems Integration; Techniques; Technology; Technology Assessment; Temperature; Testing; Thyroid Gland; Time; Traction; Tube; Universities; Utah; Vial device; Vitamin B Complex; Waste Products; Whole Blood; Work; base; care outcomes; cost; crystallinity; design; electric impedance; electrical measurement; electrical potential; experience; heart rhythm; home test; improved; miniaturize; nanopore; negative affect; point of care; point of care testing; professor; programs; prototype; response; sensor; theories

Project Summary An electrolyte panel is a blood test that measures the levels of electrolytes and carbon dioxide in your blood. Electrolytes are chemicals found naturally in the body, such as potassium, calcium, sodium, and are needed to keep the body's balance of fluids at the proper level and to maintain normal functions, such as heart rhythm, muscle contraction, and brain function. CO2 is a waste product made when the body breaks down food for energy, CO2 helps your blood stay at the right pH. Physicians may order an electrolyte panel as part of a regular health examination, to check on or diagnose a medical condition, and/or assess whether medications are negatively affecting one electrolyte levels. Electrolyte panels are currently typically carried out as laboratory test, where a blood sample is sent out to be characterized via manual ion selective electrodes (ISEs). This process takes a significant amount of time, is cost inefficient, and has issues associated with the fragility and maintenance of manual ISEs. And, while there has been the development of point-of-care or handheld electrolyte panel systems, these current systems have failed to gain widespread use or traction, due to issues with stability, storability, accuracy, and inability to be combined with other assays for comprehensive blood work analyses, i.e. complete blood count (CBC), chemistry (basic metabolic) panel, thyroid panel, nutrient tests for levels of vital nutrients, such as iron or B vitamins, enzyme markers, and STDs. Recently however, Professor Henry S. White at the University of Utah has developed a new nanopore-based ISE technology that solves the predominant issues of conventional ISEs, can be multiplexed to a high degree, can be mass fabricated and can be easily combined with other nanopore-based characterization techniques for complete blood work analyses on a single device. During this Phase 1 STTR program, Electronic BioSciences will develop and expand upon Professor White’s nanopore-based ISE technology, developing a storable, highly stable, rapid, and accurate electrolyte panel POC testing vial. The utility of this device will be demonstrated characterizing electrolyte abnormalities in blood samples from chronic kidney disease patients.

项目摘要 电解质面板是一种血液测试，测量血液中电解质和二氧化碳的水平。 电解质是人体内天然存在的化学物质，如钾、钙、钠， 保持身体的液体平衡在适当的水平，并维持正常的功能，如心脏节律， 肌肉收缩和大脑功能二氧化碳是人体分解食物时产生的废物， 能量，二氧化碳帮助你的血液保持在正确的pH值。医生可能会订购电解质面板作为常规治疗的一部分。 健康检查，检查或诊断医疗状况，和/或评估药物是否 负面地影响一种电解质水平。电解质板目前通常作为实验室测试进行， 其中血液样品被送出以通过手动离子选择性电极（ISE）进行表征。这个过程 需要大量的时间，成本效率低，并且存在与脆弱性和维护相关的问题 手动ISE。而且，虽然已经发展了即时护理或手持电解质面板， 系统，由于稳定性问题， 可储存性、准确性，以及无法与其他检测方法结合进行综合血液分析，即 全血细胞计数（CBC），化学（基本代谢）面板，甲状腺面板，营养测试的水平，重要的 营养素，如铁或B族维生素、酶标记物和性传播疾病。然而，最近，亨利教授S.白色 犹他州大学的研究人员开发了一种新的基于纳米孔的伊势技术，该技术解决了 传统ISE的问题，可以高度复用，可以大规模制造，并且可以容易地 与其他基于纳米孔的表征技术相结合，在单个设备上进行完整的血液工作分析 设备.在这个第一阶段STTR计划，电子生物科学将发展和扩大教授 白色的基于纳米孔的伊势技术，开发可储存、高度稳定、快速、精确的电解质 平板POC测试瓶。该装置的实用性将被证明是表征电解质异常， 从慢性肾病患者的血液样本中提取。