The Nanopore-based Ion Selective Electrode Vial

项目摘要 电解液测试板是一种血液测试，可以测量血液中电解质和二氧化碳的水平。 电解质是人体内天然存在的化学物质，如钾、钙、钠，是 将身体的液体平衡保持在适当的水平，并保持正常的功能，如心律， 肌肉收缩和大脑功能。二氧化碳是一种废物，当人体分解食物以 能量，二氧化碳帮助你的血液保持在合适的PH值。医生可以订购电解液面板作为常规治疗的一部分 健康检查，检查或诊断身体状况，和/或评估药物是否 对一种电解液水平产生负面影响。电解液电池板目前通常作为实验室测试进行， 血液样本通过手动离子选择电极(ISE)送出进行鉴定。这一过程 耗费大量时间，成本效益低，并且存在与脆弱性和维护相关的问题 手动ISES。虽然已经有了护理点式或手持电解液面板的发展 由于稳定性问题，这些目前的系统未能获得广泛的使用或牵引力， 可储存性、准确性以及不能与其他分析方法结合进行全面的血液分析，例如 全血计数(CBC)，化学(基础代谢)板，甲状腺板，营养测试 营养素，如铁或维生素B、酶标记物和性传播疾病。然而，最近，亨利·S·怀特教授 已经开发出一种新的基于纳米孔的ISE技术，该技术解决了主要的 传统ISE的问题可以高度多路复用，可以批量制造，并且可以很容易地 与其他基于纳米孔的表征技术相结合，在单个 设备。在这个第一阶段的STTR计划中，电子生物科学将在教授的基础上发展和扩展 White的基于纳米孔的ISE技术，开发一种可储存、高度稳定、快速和精确的电解液 面板POC测试瓶。该设备的用途将被证明表征电解质异常。 慢性肾病患者的血液样本。

Project Summary An electrolyte panel is a blood test that measures the levels of electrolytes and carbon dioxide in your blood. Electrolytes are chemicals found naturally in the body, such as potassium, calcium, sodium, and are needed to keep the body's balance of fluids at the proper level and to maintain normal functions, such as heart rhythm, muscle contraction, and brain function. CO2 is a waste product made when the body breaks down food for energy, CO2 helps your blood stay at the right pH. Physicians may order an electrolyte panel as part of a regular health examination, to check on or diagnose a medical condition, and/or assess whether medications are negatively affecting one electrolyte levels. Electrolyte panels are currently typically carried out as laboratory test, where a blood sample is sent out to be characterized via manual ion selective electrodes (ISEs). This process takes a significant amount of time, is cost inefficient, and has issues associated with the fragility and maintenance of manual ISEs. And, while there has been the development of point-of-care or handheld electrolyte panel systems, these current systems have failed to gain widespread use or traction, due to issues with stability, storability, accuracy, and inability to be combined with other assays for comprehensive blood work analyses, i.e. complete blood count (CBC), chemistry (basic metabolic) panel, thyroid panel, nutrient tests for levels of vital nutrients, such as iron or B vitamins, enzyme markers, and STDs. Recently however, Professor Henry S. White at the University of Utah has developed a new nanopore-based ISE technology that solves the predominant issues of conventional ISEs, can be multiplexed to a high degree, can be mass fabricated and can be easily combined with other nanopore-based characterization techniques for complete blood work analyses on a single device. During this Phase 1 STTR program, Electronic BioSciences will develop and expand upon Professor White’s nanopore-based ISE technology, developing a storable, highly stable, rapid, and accurate electrolyte panel POC testing vial. The utility of this device will be demonstrated characterizing electrolyte abnormalities in blood samples from chronic kidney disease patients.