BioRepository and Precision Pathology Center

生物样本库和精准病理中心

• 批准号: 10323300
• 负责人: Shannon Jones McCall
• 金额: $ 17.61万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10323300
• 关键词: Accreditation; African American; Algorithmic Analysis; Algorithmic Software; American; Animal Model; Archives; Benign; Biological; Biological Assay; Biopsy; Bladder; Blood; Breast; Budgets; Cancer Biology; Cancer Center Support Grant; Cell Line; Cells; Characteristics; Clinical; Clinical Research; Clinical Trials; Colorectal; Complement; Consent; Cryopreservation; DNA; Data; Databases; Disease; Enrollment; Ensure; Event; Fostering; Future; Generations; Genomics; Goals; Health Service Area; Health system; Histology; Image Analysis; Immunohistochemistry; In Situ Hybridization; Infrastructure; Institutes; Instruction; International; Journals; Kidney; Laboratories; Leadership; Libraries; Link; Liquid substance; Lung; Malignant - descriptor; Malignant Neoplasms; Medicine; Microdissection; Minority; Minority Participation; Molecular; Monitor; Nature; Operative Surgical Procedures; Ovarian; Paraffin; Paraffin Tissue; Participant; Pathologist; Pathology; Patients; Phase; Population; Primary Cell Cultures; Prostate; Proteomics; Protocols documentation; Publications; RNA; Reporting; Research; Research Institute; Research Personnel; Research Support; Resource Sharing; Retrieval; Retrospective Studies; Sampling; Schedule; Services; Shipping; Site; Societies; Solid; Source; Technology; The Cancer Genome Atlas; Time; Tissue Banks; Tissue Microarray; Tissue Sample; Tissues; Translational Research; Universities; Vertebral column; Veterinary Pathology; Visualization; Work; base; biobank; cancer cell; clinical trial enrollment; cohort; college; community engagement; ethical legal social implication; health equity; improved; laser capture microdissection; member; microbiome; patient derived xenograft model; patient oriented; precision oncology; programs; quantitative imaging; repository; response; sample collection; standard of care; tissue archive; tissue processing; tumor; underserved minority; waiver; whole slide imaging

ABSTRACT – BIOREPOSITORY & PRECISION PATHOLOGY CENTER SHARED RESOURCE The BioRepository & Precision Pathology Center (BRPC) provides patient tissue and blood-based research support for the Duke Cancer Institute (DCI). Major service areas are Biobanking (BRPC broad consent, tissue, blood and fluid sample procurement, processing, storage and distribution including for the BRPC’s patient- derived xenograft (PDX) and primary cell culture (PCC) service lines, tumor enrichment through macro/microdissection, DNA/RNA extraction), Regulatory (protocol review, scope of work creation, budgeting, integration with broad consent if possible), and ImmunoHistology (traditional histology, single and multiplexed immunohistochemistry, in situ hybridization, tissue microarray creation, whole slide imaging and image analysis). Regarding the Biobanking service, the BRPC administers Duke’s largest broad consent protocol for specimen collection for research, which is becoming the backbone consent for new microbiome initiatives and has consented >4,700 patients since 2012. Of these patients, 17% are African American and 4% other non- Caucasian, supporting research into cancers of underserved minorities. The BRPC also operates a College of American Pathologists’ (CAP)-accredited biorepository of normal and diseased samples representing a range of solid and hematolymphoid pathologies. Banking activities occur in conjunction with blood draws, standard-of- care biopsy and surgical procedures and clinical trial-supported tissue acquisitions. BRPC prioritizes activities based on instructions of DCI’s disease-site group leaders. Often a single biobanking event can simultaneously supply multiple ongoing research efforts, prepare a PDX model, prepare PCCs, and stock the biobank for future needs. BRPC biospecimens also are being used in Phase III of the NCI’s CPTAC project. The BRPC also serves as the administrative and regulatory backbone for the DCI’s Personalized Cancer Medicine Initiative (PCMI), which links genomic information, clinical annotation, and biospecimens. The gateway to the paraffin tissue archives in the Department of Pathology, BRPC provides regulatory support, cohort identification, and pathologist review for retrospective studies using those archives. BRPC rapidly retrieves and prepares patients’ paraffin archival tissue samples for submission to clinical trial sponsors. Finally, the BRPC provides specialized tissue processing and experimental services including basic histology, veterinary pathology support for animal models, immunohistochemistry, in situ hybridization assays, tissue microarray creation, tumor enrichment by microdissection and laser capture microdissection, whole slide imaging and image analysis algorithms. BRPC biospecimens contributed to the Cancer Genome Atlas Project have supported numerous high-impact publications in Cell, Nature, Cancer Cell, and Cell Reports. In 2018, the BRPC provided services to 177 investigators, 58% of whom were DCI members who accounted for 92% of total usage and represented all 8 DCI Research Programs. Use of BRPC by DCI Members, contributed to 170 publications over the current project period, of which 54 were in high impact journals.

摘要 - 生物验证和精密病理中心共享资源 生物疗法与精密病理中心（BRPC）提供患者组织和基于血液的研究 支持杜克癌症研究所（DCI）。主要的服务领域是生物群（BRPC广泛的同意，组织， 血液和流体样品采购，加工，存储和分布，包括BRPC患者 衍生的异种移植（PDX）和原代细胞培养（PCC）使用线，通过 宏/微分解，DNA/RNA提取），监管（协议审查，工作范围，预算范围，预算， 如果可能的话，请与广泛的同意以及免疫组织学（传统的组织学，单一和多重的组织学 免疫组织化学，原位杂交，组织微阵列创建，整个幻灯片成像和图像分析）。 关于生物库服务，BRPC管理Duke最大的标本广泛同意协议 研究收集，这已成为新微生物组计划的骨干同意，并已有 自2012年以来，同意> 4,700名患者。在这些患者中，17％是非裔美国人，其他4％的其他非美国人 高加索人支持对服务不足少数民族的癌症的研究。 BRPC还经营着一所大学 美国病理学家（CAP）认可的正常样本和解散样品的生物座位，代表一系列 固体和血小淋巴样病变。银行活动与抽血结合，标准 - 护理活检和外科手术以及临床试验的组织采集。 BRPC优先考虑活动 根据DCI疾病部门领导人的指示。通常，一个生物群体可以轻松 提供多项正在进行的研究工作，准备PDX模型，准备PCC并为生物库库存以备未来 需要。 NCI的CPTAC项目的第三阶段也使用了BRPC生物测量。 BRPC也服务 作为DCI个性化癌症医学倡议（PCMI）的行政和监管主干， 它可以连接基因组信息，临床注释和生物测量。通往石蜡组织的门户 病理学系的档案，BRPC提供监管支持，队列识别和 病理学家审查了使用这些档案的回顾性研究。 BRPC迅速检索并为患者做准备 石蜡档案组织样品以征服临床试验发起人。最后，BRPC提供专业 组织加工和实验服务，包括基本组织学，兽医病理学支持动物 模型，免疫组织化学，原位杂交测定，组织微阵列创建，肿瘤富集 显微解剖和激光捕获显微解剖，整个幻灯片成像和图像分析算法。 BRPC 为癌症基因组图集项目做出了贡献的生物测量支持了许多高影响力 细胞，自然，癌细胞和细胞报告中的出版物。 2018年，BRPC为177名调查人员提供了服务，其中58％是DCI成员 占总用法的92％，代表了所有8个DCI研究计划。 DCI成员使用BRPC， 在当前的项目期间为170个出版物做出了贡献，其中54个在高影响期刊中。