BioRepository and Precision Pathology Center

生物样本库和精准病理中心

• 批准号: 10323300
• 负责人: Shannon Jones McCall
• 金额: $ 17.61万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10323300
• 关键词: Accreditation; African American; Algorithmic Analysis; Algorithmic Software; American; Animal Model; Archives; Benign; Biological; Biological Assay; Biopsy; Bladder; Blood; Breast; Budgets; Cancer Biology; Cancer Center Support Grant; Cell Line; Cells; Characteristics; Clinical; Clinical Research; Clinical Trials; Colorectal; Complement; Consent; Cryopreservation; DNA; Data; Databases; Disease; Enrollment; Ensure; Event; Fostering; Future; Generations; Genomics; Goals; Health Service Area; Health system; Histology; Image Analysis; Immunohistochemistry; In Situ Hybridization; Infrastructure; Institutes; Instruction; International; Journals; Kidney; Laboratories; Leadership; Libraries; Link; Liquid substance; Lung; Malignant - descriptor; Malignant Neoplasms; Medicine; Microdissection; Minority; Minority Participation; Molecular; Monitor; Nature; Operative Surgical Procedures; Ovarian; Paraffin; Paraffin Tissue; Participant; Pathologist; Pathology; Patients; Phase; Population; Primary Cell Cultures; Prostate; Proteomics; Protocols documentation; Publications; RNA; Reporting; Research; Research Institute; Research Personnel; Research Support; Resource Sharing; Retrieval; Retrospective Studies; Sampling; Schedule; Services; Shipping; Site; Societies; Solid; Source; Technology; The Cancer Genome Atlas; Time; Tissue Banks; Tissue Microarray; Tissue Sample; Tissues; Translational Research; Universities; Vertebral column; Veterinary Pathology; Visualization; Work; base; biobank; cancer cell; clinical trial enrollment; cohort; college; community engagement; ethical legal social implication; health equity; improved; laser capture microdissection; member; microbiome; patient derived xenograft model; patient oriented; precision oncology; programs; quantitative imaging; repository; response; sample collection; standard of care; tissue archive; tissue processing; tumor; underserved minority; waiver; whole slide imaging

ABSTRACT – BIOREPOSITORY & PRECISION PATHOLOGY CENTER SHARED RESOURCE The BioRepository & Precision Pathology Center (BRPC) provides patient tissue and blood-based research support for the Duke Cancer Institute (DCI). Major service areas are Biobanking (BRPC broad consent, tissue, blood and fluid sample procurement, processing, storage and distribution including for the BRPC’s patient- derived xenograft (PDX) and primary cell culture (PCC) service lines, tumor enrichment through macro/microdissection, DNA/RNA extraction), Regulatory (protocol review, scope of work creation, budgeting, integration with broad consent if possible), and ImmunoHistology (traditional histology, single and multiplexed immunohistochemistry, in situ hybridization, tissue microarray creation, whole slide imaging and image analysis). Regarding the Biobanking service, the BRPC administers Duke’s largest broad consent protocol for specimen collection for research, which is becoming the backbone consent for new microbiome initiatives and has consented >4,700 patients since 2012. Of these patients, 17% are African American and 4% other non- Caucasian, supporting research into cancers of underserved minorities. The BRPC also operates a College of American Pathologists’ (CAP)-accredited biorepository of normal and diseased samples representing a range of solid and hematolymphoid pathologies. Banking activities occur in conjunction with blood draws, standard-of- care biopsy and surgical procedures and clinical trial-supported tissue acquisitions. BRPC prioritizes activities based on instructions of DCI’s disease-site group leaders. Often a single biobanking event can simultaneously supply multiple ongoing research efforts, prepare a PDX model, prepare PCCs, and stock the biobank for future needs. BRPC biospecimens also are being used in Phase III of the NCI’s CPTAC project. The BRPC also serves as the administrative and regulatory backbone for the DCI’s Personalized Cancer Medicine Initiative (PCMI), which links genomic information, clinical annotation, and biospecimens. The gateway to the paraffin tissue archives in the Department of Pathology, BRPC provides regulatory support, cohort identification, and pathologist review for retrospective studies using those archives. BRPC rapidly retrieves and prepares patients’ paraffin archival tissue samples for submission to clinical trial sponsors. Finally, the BRPC provides specialized tissue processing and experimental services including basic histology, veterinary pathology support for animal models, immunohistochemistry, in situ hybridization assays, tissue microarray creation, tumor enrichment by microdissection and laser capture microdissection, whole slide imaging and image analysis algorithms. BRPC biospecimens contributed to the Cancer Genome Atlas Project have supported numerous high-impact publications in Cell, Nature, Cancer Cell, and Cell Reports. In 2018, the BRPC provided services to 177 investigators, 58% of whom were DCI members who accounted for 92% of total usage and represented all 8 DCI Research Programs. Use of BRPC by DCI Members, contributed to 170 publications over the current project period, of which 54 were in high impact journals.

摘要 – 生物样本库和精准病理中心共享资源 生物储存库和精密病理学中心 (BRPC) 提供基于患者组织和血液的研究 对杜克癌症研究所 (DCI) 的支持。主要服务领域是生物样本库（BRPC 广泛同意、组织、 血液和体液样本的采购、处理、储存和分发，包括 BRPC 患者的血液和体液样本的采集、处理、储存和分发 衍生异种移植（PDX）和原代细胞培养（PCC）服务线，通过肿瘤富集 宏观/显微解剖、DNA/RNA 提取）、监管（方案审查、工作创建范围、预算、 如果可能的话，在获得广泛同意的情况下进行整合）和免疫组织学（传统组织学，单一和多重组织学） 免疫组织化学、原位杂交、组织微阵列创建、全玻片成像和图像分析）。 关于生物样本库服务，BRPC 管理杜克大学最大的样本广泛同意协议 研究收集，这正在成为新的微生物组计划的主要同意，并且已经 自 2012 年以来，同意超过 4,700 名患者。在这些患者中，17% 是非裔美国人，4% 是其他非裔美国人 白种人，支持对服务不足的少数族裔的癌症研究。 BRPC 还运营一所学院 美国病理学家 (CAP) 认可的生物样本库，包含代表一系列正常和患病样本的样本 实体病和血淋巴病。银行活动与抽血同时进行，标准 护理活检和外科手术以及临床试验支持的组织采集。 BRPC 确定活动的优先顺序 根据 DCI 疾病现场小组领导的指示。通常，单个生物样本库事件可以同时进行 提供多项正在进行的研究工作、准备 PDX 模型、准备 PCC 并为未来储备生物样本库 需要。 BRPC 生物样本也被用于 NCI 的 CPTAC 项目的第三阶段。 BRPC 还服务于 作为 DCI 个性化癌症医学计划 (PCMI) 的行政和监管支柱， 它将基因组信息、临床注释和生物样本联系起来。通往石蜡组织的大门 BRPC 在病理科档案室提供监管支持、队列识别和 病理学家使用这些档案进行回顾性研究审查。 BRPC 快速检索并准备患者的信息 石蜡档案组织样本提交给临床试验申办者。最后，BRPC 提供了专门的 组织加工和实验服务，包括基础组织学、动物病理学支持 模型、免疫组织化学、原位杂交测定、组织微阵列创建、肿瘤富集 显微切割和激光捕获显微切割、全玻片成像和图像分析算法。 BRPC 为癌症基因组图谱项目贡献的生物样本支持了许多高影响力 在《Cell》、《Nature》、《Cancer Cell》和《Cell Reports》上发表论文。 2018 年，BRPC 为 177 名研究者提供了服务，其中 58% 为 DCI 成员，占 占总使用量的 92%，代表所有 8 个 DCI 研究计划。 DCI 成员对 BRPC 的使用， 在当前项目期间发表了 170 篇出版物，其中 54 篇发表在高影响力期刊上。