Core D: Mouse Genetics and Genomics Core

总结/摘要 该计划中的所有项目将广泛使用基因操作小鼠作为主要的 实验平台小鼠遗传学和基因组学核心（核心D）是 为所有4个项目以及科学核心B和C提供关键支持的计划。核心将利用 在爱荷华州大学建立的基因编辑设施的基础设施，用于产生遗传 使用CRISPR-Cas9基因组编辑和爱荷华州研究所基因组学部门的改变小鼠模型 人类遗传学（IIHG），以支持全基因组转录组分析。核心的目标是：1） 提供转基因和基因敲除动物的集中育种、基因分型和质量控制， 研究人员在该计划项目，2）产生新的转基因小鼠模型需要在拟议的 项目2和项目4的预算期间（以及项目3的未来），以及3）执行和分析全球 项目1-4中提出的转录组研究。

Summary/Abstract All projects in this program will make extensive use of genetically manipulated mice as the primary experimental platform. The Mouse Genetics and Genomics Core (Core D) is an essential component of the Program providing critical support to all 4 projects and to scientific cores B and C. The Core will capitalize on the infrastructure established at the University of Iowa Gene Editing Facility for the generation of genetically altered mouse models using CRISPR-Cas9 genome editing and in the Genomics Division of the Iowa Institute of Human Genetics (IIHG) to support genome wide transcriptome analysis. The goals of the Core are to: 1) provide centralized breeding, genotyping and quality control of transgenic and knockout animals for use by investigators in the program project, 2) generate new transgenic mouse models needed during the proposed budget period for Projects 2 and 4 (and in the future for Project 3), and 3) perform and analyze global transcriptome studies proposed within Projects 1-4.