TAS::75 0849::TAS INCREMENTAL FUNDING

塔斯马尼亚州::75 0849::塔斯马尼亚州增量资金

• 批准号: 10329736
• 负责人: LEONARD FREEDMAN
• 金额: $ 0.35万
• 依托单位: LEIDOS BIOMEDICAL RESEARCH, INC.
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-31 至 2021-08-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10329736
• 关键词: Adherence; Aliquot; Animals; Biological; Blood; Blood specimen; Breast Cancer Risk Factor; CCR; Cancerous; Cell Culture Techniques; Cell Proliferation; Cells; Cervical; Clinical; Clinical Trials; Collection; Colon Carcinoma; Communication; DNA; Data Analyses; Databases; Diet; Differentiation and Growth; Flavonoids; Food; Freezing; Funding; Future; Generations; Genistein; Human; Human Resources; Imaging technology; In Vitro; Indole-3-Carbinol; Inflammation; Inflammatory; Joints; Laboratories; Leukocytes; Liquid substance; Maintenance; Measurement; Messenger RNA; Monitor; National Surgical Adjuvant Breast and Bowel Project; Natural regeneration; Nitrogen; Normal Cell; Physiological; Plasma; Process; Proteomics; Research; Resveratrol; Retrieval; Risk Behaviors; Rodent Model; Role; Sampling; Scientist; Services; Shipping; Specific qualifier value; Specimen; Structure of nail of toe; Sulfur; Technology; Testing; Tissue Sample; Tumor Stem Cells; Vial device; Visit; Woman; bean; biobank; men; neoplastic cell; repository; statistical center; stem cell differentiation; stem cells; transcriptomics; tumor; tumor behavior

Support is part of a joint research initiative with CCR (Farrar Laboratory) to examine the role of various food components as modifiers of the behavior of tumor stem cells and ultimately on tumor regeneration. These in vitro studies use cell culture technologies to evaluate the influence of physiologically relevant concentrations of food components on the differentiation of cancerous and non-cancerous stem cells. Several biological measurements are made to evaluate the impact of indole 3-carbinol, genistein, resveratrol, allyl sulfur, etc. on stem cell differentiation and growth potential. This support is part of a joint research initiative with CCR (Drs. Timothy Veenstra and Thomas Turbyville Laboratories) to examine use proteomic technologies to evaluate the role of various food components as modifiers of breast cancer risk and tumor behavior. Both in vitro studies using cells in culture and animal studies will be used to evaluate food components. Initially studies will focus on genistein as a modifier of both normal and tumor cells in culture. Future studies will evaluate the influence of indole 3-carbinol, resveratrol on shifts in proteomics and how these relate to cell proliferation. This support is part of a joint research initiative with CCR (Colburn Laboratory) to examine the role of various food components as modifiers of inflammation and ultimately the impact on colon cancer. Animal studies which incorporate imaging technologies are used to monitor the impact of diet. Several biological measurements are made to evaluate the impact of indole 3-carbinol, genistein, resveratrol, flavonoids in beans, etc. on the inflammatory process. Fisher Bio Services receives clinical samples from contributing labs, aliquots specified samples to construct reference sets, logs identifiers for each sample, into their database, maintains freezers all these samples and sends out reference sets to scientists approved for testing. This will be an ongoing project as long as samples remain in the freezers. Storage and retrieval as needed of blood and cervical cell samples that were collected during clinical trial � ALTS. Cervical vials are stored in a cold room. Other blood and cervical samples are stored in liquid nitrogen freezers. This support is to reimburse Frederick for completing transcriptomic (mRNA) analysis from samples from human being and rodent models. Blood and tissue samples will be made available to those conducting the transcriptomic analysis. Most of the samples provided will require initial extraction. The data from these analyses are provided to us electronically for independent analysis. The SELECT Biorepository is a collection of blood, toenails, white cells, and plasma from the SELECT clinical trial involving over 35,000 men. The ongoing activities include processing blood on 2500 men seen on transition visits, storage of all the bloods since the study initiation in 2000, weekly generation of BSI files, ongoing communication with SELECT Statistical Center personnel, shipping samples fro the adherence sub-study, DNA extraction for roughly 400 specimens. The NSABP Repository for STAR includes ongoing storage of specimens and the maintenance of the freezers that store blood, white cells and plasma on over 19,000 women on the STAR clinical trial. The tasks involve regular checks of specimens and freezers, accession of specimens, record storage, and interaction with the NSABP.

支持是与CCR（Farrar实验室）联合研究计划的一部分，以研究各种食物成分作为肿瘤干细胞行为修饰剂的作用，并最终影响肿瘤再生。这些体外研究使用细胞培养技术来评估食物成分的生理相关浓度对癌性和非癌性干细胞分化的影响。采用生物学方法评价吲哚-3-甲醇、染料木素、白藜芦醇、烯丙基硫等对干细胞分化和生长潜能的影响。这项支持是与CCR（Timothy Veenstra博士和托马斯特比维尔实验室）联合研究计划的一部分，旨在研究使用蛋白质组学技术评估各种食物成分作为乳腺癌风险和肿瘤行为调节剂的作用。使用培养细胞的体外研究和动物研究都将用于评估食品成分。最初的研究将集中在染料木黄酮作为一种修饰剂的正常和肿瘤细胞的文化。未来的研究将评估吲哚-3-甲醇、白藜芦醇对蛋白质组学变化的影响，以及这些变化与细胞增殖的关系。这项支持是与CCR（科尔本实验室）联合研究计划的一部分，旨在研究各种食物成分作为炎症调节剂的作用，并最终对结肠癌的影响。结合成像技术的动物研究用于监测饮食的影响。进行了几种生物学测量以评估吲哚3-甲醇、染料木素、白藜芦醇、豆类中的类黄酮等对炎症过程的影响。Fisher Bio Services从贡献实验室接收临床样本，等分指定样本以构建参考集，将每个样本的标识符记录到其数据库中，保存所有这些样本的冷冻箱，并将参考集发送给批准进行检测的科学家。只要样品仍在冰柜中，这将是一个持续的项目。根据需要储存和检索在临床试验期间收集的血液和宫颈细胞样本。宫颈小瓶储存在冷藏室中。其他血液和宫颈样本储存在液氮冷冻箱中。这项支持是为了补偿Frederick完成人类和啮齿动物模型样本的转录组学（mRNA）分析。血液和组织样本将提供给进行转录组分析的人员。所提供的大多数样品都需要进行初步提取。这些分析的数据以电子方式提供给我们进行独立分析。SELECT Biorepository是来自SELECT临床试验的血液、脚趾甲、白色细胞和血浆的集合，该临床试验涉及超过35，000名男性。正在进行的活动包括处理2500例接受过渡访视的男性的血液，储存自2000年研究开始以来的所有血液，每周生成BSI文件，与SELECT统计中心人员进行持续沟通，运输依从性子研究的样本，提取约400份标本的DNA。星星的NSABP储存库包括正在进行的标本储存和对储存星星临床试验中19 000多名妇女的血液、白色细胞和血浆的冰柜的维护。这些任务包括定期检查标本和冷冻箱、标本登记、记录储存以及与国家生物安全局的互动。