TAS::75 0849::TAS INCREMENTAL FUNDING

塔斯马尼亚州::75 0849::塔斯马尼亚州增量资金

• 批准号: 10329736
• 负责人: LEONARD FREEDMAN
• 金额: $ 0.35万
• 依托单位: LEIDOS BIOMEDICAL RESEARCH, INC.
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-31 至 2021-08-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10329736
• 关键词: Adherence; Aliquot; Animals; Biological; Blood; Blood specimen; Breast Cancer Risk Factor; CCR; Cancerous; Cell Culture Techniques; Cell Proliferation; Cells; Cervical; Clinical; Clinical Trials; Collection; Colon Carcinoma; Communication; DNA; Data Analyses; Databases; Diet; Differentiation and Growth; Flavonoids; Food; Freezing; Funding; Future; Generations; Genistein; Human; Human Resources; Imaging technology; In Vitro; Indole-3-Carbinol; Inflammation; Inflammatory; Joints; Laboratories; Leukocytes; Liquid substance; Maintenance; Measurement; Messenger RNA; Monitor; National Surgical Adjuvant Breast and Bowel Project; Natural regeneration; Nitrogen; Normal Cell; Physiological; Plasma; Process; Proteomics; Research; Resveratrol; Retrieval; Risk Behaviors; Rodent Model; Role; Sampling; Scientist; Services; Shipping; Specific qualifier value; Specimen; Structure of nail of toe; Sulfur; Technology; Testing; Tissue Sample; Tumor Stem Cells; Vial device; Visit; Woman; bean; biobank; men; neoplastic cell; repository; statistical center; stem cell differentiation; stem cells; transcriptomics; tumor; tumor behavior

Support is part of a joint research initiative with CCR (Farrar Laboratory) to examine the role of various food components as modifiers of the behavior of tumor stem cells and ultimately on tumor regeneration. These in vitro studies use cell culture technologies to evaluate the influence of physiologically relevant concentrations of food components on the differentiation of cancerous and non-cancerous stem cells. Several biological measurements are made to evaluate the impact of indole 3-carbinol, genistein, resveratrol, allyl sulfur, etc. on stem cell differentiation and growth potential. This support is part of a joint research initiative with CCR (Drs. Timothy Veenstra and Thomas Turbyville Laboratories) to examine use proteomic technologies to evaluate the role of various food components as modifiers of breast cancer risk and tumor behavior. Both in vitro studies using cells in culture and animal studies will be used to evaluate food components. Initially studies will focus on genistein as a modifier of both normal and tumor cells in culture. Future studies will evaluate the influence of indole 3-carbinol, resveratrol on shifts in proteomics and how these relate to cell proliferation. This support is part of a joint research initiative with CCR (Colburn Laboratory) to examine the role of various food components as modifiers of inflammation and ultimately the impact on colon cancer. Animal studies which incorporate imaging technologies are used to monitor the impact of diet. Several biological measurements are made to evaluate the impact of indole 3-carbinol, genistein, resveratrol, flavonoids in beans, etc. on the inflammatory process. Fisher Bio Services receives clinical samples from contributing labs, aliquots specified samples to construct reference sets, logs identifiers for each sample, into their database, maintains freezers all these samples and sends out reference sets to scientists approved for testing. This will be an ongoing project as long as samples remain in the freezers. Storage and retrieval as needed of blood and cervical cell samples that were collected during clinical trial � ALTS. Cervical vials are stored in a cold room. Other blood and cervical samples are stored in liquid nitrogen freezers. This support is to reimburse Frederick for completing transcriptomic (mRNA) analysis from samples from human being and rodent models. Blood and tissue samples will be made available to those conducting the transcriptomic analysis. Most of the samples provided will require initial extraction. The data from these analyses are provided to us electronically for independent analysis. The SELECT Biorepository is a collection of blood, toenails, white cells, and plasma from the SELECT clinical trial involving over 35,000 men. The ongoing activities include processing blood on 2500 men seen on transition visits, storage of all the bloods since the study initiation in 2000, weekly generation of BSI files, ongoing communication with SELECT Statistical Center personnel, shipping samples fro the adherence sub-study, DNA extraction for roughly 400 specimens. The NSABP Repository for STAR includes ongoing storage of specimens and the maintenance of the freezers that store blood, white cells and plasma on over 19,000 women on the STAR clinical trial. The tasks involve regular checks of specimens and freezers, accession of specimens, record storage, and interaction with the NSABP.

这项支持是与 CCR（Farrar 实验室）联合研究计划的一部分，旨在检查各种食物成分作为肿瘤干细胞行为调节剂以及最终对肿瘤再生的作用。这些体外研究使用细胞培养技术来评估食品成分的生理相关浓度对癌性和非癌性干细胞分化的影响。进行了多项生物学测量来评估吲哚3-甲醇、染料木黄酮、白藜芦醇、烯丙基硫等对干细胞分化和生长潜力的影响。这项支持是与 CCR（Timothy Veenstra 博士和 Thomas Turbyville Laboratories）联合研究计划的一部分，该计划旨在研究使用蛋白质组学技术来评估各种食品成分作为乳腺癌风险和肿瘤行为调节剂的作用。使用培养细胞的体外研究和动物研究都将用于评估食品成分。最初的研究将集中于金雀异黄素作为培养物中正常细胞和肿瘤细胞的修饰剂。未来的研究将评估吲哚 3-甲醇、白藜芦醇对蛋白质组学变化的影响以及它们与细胞增殖的关系。这项支持是与 CCR（科尔伯恩实验室）联合研究计划的一部分，旨在研究各种食物成分作为炎症调节剂的作用以及最终对结肠癌的影响。结合成像技术的动物研究用于监测饮食的影响。通过多项生物学测量来评估吲哚 3-甲醇、染料木黄酮、白藜芦醇、豆类中的类黄酮等对炎症过程的影响。 Fisher Bio Services 从贡献实验室接收临床样本，等分指定样本以构建参考集，将每个样本的标识符记录到数据库中，维护冷冻所有这些样本并将参考集发送给批准进行测试的科学家。只要样品仍留在冰箱中，这将是一个持续的项目。根据需要存储和检索临床试验期间收集的血液和宫颈细胞样本 – ALTS。宫颈瓶储存在冷藏室中。其他血液和宫颈样本储存在液氮冷冻柜中。这项支持是为了补偿 Frederick 完成人类和啮齿动物模型样本的转录组 (mRNA) 分析。血液和组织样本将提供给进行转录组分析的人员。提供的大多数样品都需要进行初步提取。这些分析的数据以电子方式提供给我们进行独立分析。 SELECT 生物储存库收集了 SELECT 临床试验的血液、脚趾甲、白细胞和血浆，涉及超过 35,000 名男性。正在进行的活动包括处理 2500 名过渡访问男性的血液、储存自 2000 年研究启动以来的所有血液、每周生成 BSI 文件、与 SELECT 统计中心人员持续沟通、运送依从性子研究的样本、提取大约 400 个样本的 DNA。 STAR 的 NSABP 存储库包括样本的持续存储和冰箱的维护，这些冰箱储存了 STAR 临床试验中超过 19,000 名女性的血液、白细胞和血浆。这些任务包括定期检查标本和冰箱、标本加入、记录存储以及与 NSABP 的互动。