TAS::75 0849::TAS INCREMENTAL FUNDING

塔斯马尼亚州::75 0849::塔斯马尼亚州增量资金

• 批准号: 10329736
• 负责人: LEONARD FREEDMAN
• 金额: $ 0.35万
• 依托单位: LEIDOS BIOMEDICAL RESEARCH, INC.
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-31 至 2021-08-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10329736
• 关键词: Adherence; Aliquot; Animals; Biological; Blood; Blood specimen; Breast Cancer Risk Factor; CCR; Cancerous; Cell Culture Techniques; Cell Proliferation; Cells; Cervical; Clinical; Clinical Trials; Collection; Colon Carcinoma; Communication; DNA; Data Analyses; Databases; Diet; Differentiation and Growth; Flavonoids; Food; Freezing; Funding; Future; Generations; Genistein; Human; Human Resources; Imaging technology; In Vitro; Indole-3-Carbinol; Inflammation; Inflammatory; Joints; Laboratories; Leukocytes; Liquid substance; Maintenance; Measurement; Messenger RNA; Monitor; National Surgical Adjuvant Breast and Bowel Project; Natural regeneration; Nitrogen; Normal Cell; Physiological; Plasma; Process; Proteomics; Research; Resveratrol; Retrieval; Risk Behaviors; Rodent Model; Role; Sampling; Scientist; Services; Shipping; Specific qualifier value; Specimen; Structure of nail of toe; Sulfur; Technology; Testing; Tissue Sample; Tumor Stem Cells; Vial device; Visit; Woman; bean; biobank; men; neoplastic cell; repository; statistical center; stem cell differentiation; stem cells; transcriptomics; tumor; tumor behavior

Support is part of a joint research initiative with CCR (Farrar Laboratory) to examine the role of various food components as modifiers of the behavior of tumor stem cells and ultimately on tumor regeneration. These in vitro studies use cell culture technologies to evaluate the influence of physiologically relevant concentrations of food components on the differentiation of cancerous and non-cancerous stem cells. Several biological measurements are made to evaluate the impact of indole 3-carbinol, genistein, resveratrol, allyl sulfur, etc. on stem cell differentiation and growth potential. This support is part of a joint research initiative with CCR (Drs. Timothy Veenstra and Thomas Turbyville Laboratories) to examine use proteomic technologies to evaluate the role of various food components as modifiers of breast cancer risk and tumor behavior. Both in vitro studies using cells in culture and animal studies will be used to evaluate food components. Initially studies will focus on genistein as a modifier of both normal and tumor cells in culture. Future studies will evaluate the influence of indole 3-carbinol, resveratrol on shifts in proteomics and how these relate to cell proliferation. This support is part of a joint research initiative with CCR (Colburn Laboratory) to examine the role of various food components as modifiers of inflammation and ultimately the impact on colon cancer. Animal studies which incorporate imaging technologies are used to monitor the impact of diet. Several biological measurements are made to evaluate the impact of indole 3-carbinol, genistein, resveratrol, flavonoids in beans, etc. on the inflammatory process. Fisher Bio Services receives clinical samples from contributing labs, aliquots specified samples to construct reference sets, logs identifiers for each sample, into their database, maintains freezers all these samples and sends out reference sets to scientists approved for testing. This will be an ongoing project as long as samples remain in the freezers. Storage and retrieval as needed of blood and cervical cell samples that were collected during clinical trial � ALTS. Cervical vials are stored in a cold room. Other blood and cervical samples are stored in liquid nitrogen freezers. This support is to reimburse Frederick for completing transcriptomic (mRNA) analysis from samples from human being and rodent models. Blood and tissue samples will be made available to those conducting the transcriptomic analysis. Most of the samples provided will require initial extraction. The data from these analyses are provided to us electronically for independent analysis. The SELECT Biorepository is a collection of blood, toenails, white cells, and plasma from the SELECT clinical trial involving over 35,000 men. The ongoing activities include processing blood on 2500 men seen on transition visits, storage of all the bloods since the study initiation in 2000, weekly generation of BSI files, ongoing communication with SELECT Statistical Center personnel, shipping samples fro the adherence sub-study, DNA extraction for roughly 400 specimens. The NSABP Repository for STAR includes ongoing storage of specimens and the maintenance of the freezers that store blood, white cells and plasma on over 19,000 women on the STAR clinical trial. The tasks involve regular checks of specimens and freezers, accession of specimens, record storage, and interaction with the NSABP.

支持是与CCR(Farrar实验室)联合研究计划的一部分，目的是研究各种食物成分作为肿瘤干细胞行为调节剂的作用，并最终影响肿瘤再生。这些体外研究使用细胞培养技术来评估生理上相关的食物成分浓度对癌症和非癌症干细胞分化的影响。对吲哚-3-甲醇、染料木素、白藜芦醇、烯丙基硫等对干细胞分化和生长潜能的影响进行了多项生物学测量。这项支持是与CCR(Timothy Veenstra博士和Thomas Turbyville实验室)联合开展的一项研究计划的一部分，该计划旨在利用蛋白质组学技术来评估各种食品成分作为乳腺癌风险和肿瘤行为修饰物的作用。使用培养细胞的体外研究和动物研究都将被用来评估食物成分。最初的研究将集中在金雀异黄素作为正常细胞和肿瘤细胞培养的修饰剂。未来的研究将评估吲哚3-甲醇、白藜芦醇对蛋白质组学变化的影响，以及这些变化与细胞增殖的关系。这项支持是与CCR(科尔本实验室)联合研究计划的一部分，目的是研究各种食物成分作为炎症调节剂的作用，最终对结肠癌的影响。结合成像技术的动物研究被用来监测饮食的影响。对吲哚-3-甲醇、染料木素、白藜芦醇、豆类黄酮类化合物等在炎症过程中的作用进行了多项生物学测量。Fisher Bio Services从贡献实验室接收临床样本，等量指定样本以构建参考集，将每个样本的识别符记录到他们的数据库中，维护所有这些样本，并将参考集发送给经批准进行测试的科学家。只要样品还在冰柜里，这将是一个正在进行的项目。根据需要存储和检索在�ALTS临床试验期间收集的血液和宫颈细胞样本。宫颈试管存放在冷藏室中。其他血液和宫颈样本保存在液氮冷冻箱中。这项支持是为了补偿弗雷德里克完成了从人类和啮齿动物模型样本中进行的转录(MRNA)分析。血液和组织样本将提供给进行转录分析的人。所提供的大部分样品都需要初步提取。这些分析的数据以电子方式提供给我们，供我们进行独立分析。精选生物资料库是从精选临床试验中收集的血液、脚趾甲、白细胞和血浆，涉及超过35,000名男性。正在进行的活动包括处理2500名在过渡时期访问中看到的男子的血液，储存自2000年研究开始以来的所有血液，每周生成BSI文件，与选定的统计中心人员进行持续沟通，为依从性分研究运送样本，为大约400个样本提取DNA。STAR的NSABP存储库包括对STAR临床试验中19,000多名女性的样本进行持续存储，以及对存储血液、白细胞和血浆的冷冻箱进行维护。这些任务包括定期检查标本和冰柜、加入标本、记录存储以及与NSABP的互动。