Membrane Anchored-Probes for Sensing Analytes at the Cell Surface

用于感测细胞表面分析物的膜锚定探针

• 批准号: 10459398
• 负责人: Mirna El Khatib
• 金额: $ 14.89万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-02 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10459398
• 关键词: Affect; Area; Award; Benign; Bone Marrow; Calibration; Cancer Biology; Cell Culture Techniques; Cell Cycle; Cell Proliferation; Cell Survival; Cell membrane; Cell surface; Cells; Cessation of life; Confocal Microscopy; Development; Disease Progression; Drops; Elements; Endocytosis; Environment; Environmental Risk Factor; Extracellular Space; Foundations; Goals; Hematopoietic Stem Cell Transplantation; Hematopoietic stem cells; Hydrophobicity; Image; Imaging technology; In Vitro; Label; Laboratories; Learning; Leukemic Cell; Location; Malignant - descriptor; Measurement; Measures; Membrane; Metalloporphyrins; Methods; MicroRNAs; Microscopy; Modification; Molecular; Molecular Probes; Monitor; National Heart, Lung, and Blood Institute; Nutrient; Oxygen; Oxygen Consumption; Phase; Phototoxicity; Play; Porphyrins; Proliferating; Property; Recurrence; Research Personnel; Research Project Grants; Resistance; Role; Signaling Molecule; Solid; Specificity; Tail; Time; Toxic effect; Training; arm; base; biological research; biomedical imaging; cellular imaging; chemotherapy; design; effective therapy; experimental study; extracellular; hematopoietic stem cell fate; hydrophilicity; imaging probe; improved; in vivo; interest; leukemia; leukemic stem cell; leukemic transformation; phosphorescence; pre-clinical research; prevent; quantitative imaging; sensor; serial imaging; skills; stem cell niche; stem cells; synthetic construct; therapy outcome; tool; two-photon

Project Summary. Mirna El Khatib is a synthetic organic chemist, whose goal is to transition into the area of biomedical imaging with applications in cancer biology. An NHLBI K25 award will help her to accomplish this transition and simultaneously will lead to exploration and development of a new class of imaging probe, namely Membrane-Anchored Probes (MAP) for oxygen. Longitudinal imaging is an indispensible tool for learning about progression of disease and outcomes of therapies. The first step in this proposal is to develop a general approach to membrane-tethered molecular sensors. Membrane-anchored probes (MAP) offer a unique opportunity to longitudinally image concentrations of nutrients and signaling molecules, in this case oxygen, in the immediate environment of the cell. When used in vivo such probes will remain longer in the region of interest, compared to sensors dissolved in the extracellular milieu. The new MAP for oxygen will be applied towards longitudinal oxygen measurements in the niche of transplanted hematopoietic stem cells (HSCs) and leukemic cells (LCs). This will unravel why HSCs choose to localize in specific regions of the bone marrow (BM) and why leukemic stem cells (LSCs) evade death during chemotherapy.

项目总结。Mirna El Khatib是一名合成有机化学家，她的目标是过渡到