A novel passive sampler to assess personal exposures to airborne biological agents

一种新型被动采样器，用于评估个人接触空气中生物制剂的情况

• 批准号: 10647398
• 负责人: GEDIMINAS MAINELIS
• 金额: $ 22.18万
• 依托单位: RUTGERS, THE STATE UNIV OF N.J.
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2025-08-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10647398
• 关键词: novel; passive; sampler; assess; personal

A novel passive sampler to assess personal exposures to airborne biological agents This grant application responds to PAR-18-798: NIOSH Exploratory/Developmental Grant Program (R21). We propose developing and testing a qualitatively new personal sampling device to monitor personal exposures to bioaerosols without needing a pump or power source while being lightweight, unobtrusive, and cheap. These advances are enabled by using novel materials and innovative sampler design. In addition, this novel personal sampling concept is strongly supported by preliminary data. We hypothesize that Personal Rutgers Electrostatic Passive Sampler (P-REPS) will have a sampling performance comparable to existing active personal samplers while offering light weight, small footprint, low cost, absence of pumps or power supplies, and sampling timeframes as long as days and even weeks. The sampler will help us better understand personal exposures to airborne biological agents and the transmission of those agents in occupational environments. P-REPS will use an electrostatic field created by specially configured poly(vinylidene fluoride) (PVDF) film, a permanently polarized ferroelectric polymer, to capture bioaerosol particles. The PVDF film will be wound in a spiral configuration so that the film surfaces with opposing polarization face one another and create a strong electrostatic field. The electrostatic field between the film layers and its fringing effect will attract bioaerosol and deposit them onto the film for post-sampling elution and analysis. The film will be secured in a 3D-printed frame, protective housing, and a slotted umbrella-shaped cap. Once the sampler is manufactured, it will be tested in chamber studies against an active personal sampler using several airborne microorganisms. The organisms captured by both samplers will be examined using microscopy, culturing, and ATP analysis and their quantity will be compared. The data will be used to calculate the Equivalent Sampling Rate (ESR) of P-REPS – a flow rate at which a filter sampler would capture the same number of microorganisms. Next, P-REPS will be tested against a reference sampler in several field environments with different microorganism concentrations. Here, both samplers will be placed on rotating manikins and also worn by study personnel. Our goal is to achieve ESR similar to or better than the flow rate of an active personal filter-based sampler, such as the IOM sampler. The proposal responds to NIOSH Strategic Plan’s “Immune, Infectious, and Dermal Disease Prevention” cross-sector and sectors of National Agriculture, Forestry, and Fishing; Healthcare; Services; Transportation, Warehousing, and Utilities. This proposal responds to the NIOSH r2P initiative by developing an innovative personal sampler to determine personal exposures to bioaerosols, especially over long time frames, thus leading to worker health protection. The output of the study will be a prototype P-REPS for determining personal exposures to respiratory agents in occupational environments. We expect that intermediate outcomes will include a patent, citations in the literature, and, finally, the adaptation of the sampler in work environments. The end outcome is expected to be a better understanding of personal exposure to bioaerosols in workplaces.

一种评估个人暴露于空气中生物制剂的新型被动采样器 此补助金申请响应PAR-18-798：NIOSH探索/发展补助金计划（R21）。我们 建议开发和测试一种新的个人采样装置，以监测个人接触 生物气溶胶不需要泵或电源，同时重量轻、不显眼且便宜。这些 通过使用新的材料和创新的采样器设计实现了进步。此外，这部小说的个人 初步数据有力地支持了抽样概念。我们假设个人罗格斯静电 被动采样器（P-REPS）的采样性能将与现有的主动个人采样器相当 同时提供重量轻、占地面积小、成本低、没有泵或电源以及采样 时间跨度长达数天甚至数周采样器将帮助我们更好地了解个人暴露于 空气传播的生物制剂以及这些制剂在职业环境中的传播。 P-REPS将使用一种由特殊配置的聚偏氟乙烯（PVDF）薄膜产生的静电场， 永久极化的铁电聚合物，以捕获生物气溶胶颗粒。PVDF薄膜将缠绕在 螺旋配置，使得具有相反偏振的膜表面彼此面对，并产生强的 静电场膜层之间的静电场及其边缘效应将吸引生物气溶胶， 将它们存款在膜上用于后采样洗脱和分析。这部电影将被固定在一个3D打印的框架中， 保护外壳和开槽的伞形帽。一旦采样器被制造出来， 使用几种空气中的微生物对主动个人采样器进行室内研究。生物体 将使用显微镜、培养和ATP分析来检查两个采样器捕获的细菌及其数量 将被比较。这些数据将用于计算P-REPS - a流的等效采样率（ESR） 过滤取样器捕获相同数量微生物的速率。接下来，将测试P-REPS 在不同微生物浓度的几个现场环境中与参考采样器进行比较。在这里， 两个采样器将放置在旋转人体模型上，研究人员也将佩戴。我们的目标是实现ESR 类似于或优于基于有源个人过滤器的采样器（例如IOM采样器）的流速。 该提案响应了NIOSH战略计划的“免疫，传染病和皮肤病预防” 国家农业、林业和渔业的跨部门和部门;医疗保健;服务;运输， 仓储和公用事业。该提案响应NIOSH r2 P倡议，开发了一种创新的 个人采样器，以确定个人暴露于生物气溶胶，特别是在很长一段时间内，从而导致 工人健康保护。研究的结果将是一个原型P-REPS，用于确定个人 职业环境中呼吸道病原体的暴露。我们预计中期成果将包括 专利，文献中的引用，以及最后，采样器在工作环境中的适应。年底 预计成果将是更好地了解工作场所生物气溶胶的个人接触情况。