Design/Advanced Electrostatic Sampler for Total Bioaerosols

设计/先进的总生物气溶胶静电采样器

• 批准号: 7017277
• 负责人: GEDIMINAS MAINELIS
• 金额: $ 17.56万
• 依托单位: RUTGERS, THE STATE UNIV OF N.J.
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7017277
• 关键词: Fungi; air pollution; air sampling /monitoring; bacteria; biohazard detection; biomedical equipment development; environmental exposure; field study; hydropathy; ionic bond; microorganism; monitoring device; occupational hazard; polymerase chain reaction

DESCRIPTION (provided by applicant): To improve assessment and control to airborne microorganism exposure, and to protect the populations and resources at risk from airborne microbial agents advanced bioaerosol detection and sensing systems are needed. Commonly used bioaerosol samplers often measure viable bioaerosols which represent only a fraction of airborne microbial flora. In this research, responding to PA-04-030 "NIOSH Exploratory Developmental Grant Program (R21)", we propose to design, construct and evaluate a novel bioaerosol sampler which will improve our ability to determine exposures to total (viable and non-viable) airborne microorganisms. The proposed sampler will be compact, have low power consumption and, most importantly, will feature very high air sample concentration rate (1 million and higher). Such a high sample concentration rate is currently achieve by few other samplers and will significantly improve our ability to detect low airborne microorganism concentrations. In the proposed sampler, the airborne particles will be electrostatically deposited on a superhydrophobic surface ("Lotus leaf " type) from which they will be removed and collected by tiny (10-50 mu L) rolling liquid droplets, e.g. a phenomenon known as self-cleaning. The liquid droplets containing removed microorganisms will then be available for analysis by modern microbiological techniques, such as Real-Time Polymerase Chain Reaction (RT-PCR). The new sampler's overall performance (sample collection, extraction, and its subsequent analysis by RT-PCR) when collecting test bacteria and fungi will be evaluated in laboratory and field settings and against other samplers. The development of the electrostatic collector with superhydrophobic collection surface will improve our ability to determine exposure to airborne microorganisms in residential, occupational and environmental settings, thus improving our ability to protect populations are risk. The use of small collection liquid quantities will make the sampler compatible with various "laboratories on a chip" and may lead to near real-time determination of airborne microbial contaminants. In addition, use of electrostatic technique as a particle removal mechanism will enable collection and detection of airborne viruses, such as SARS or monkey pox. The research findings and practical recommendations developed in this research will be disseminated to stakeholders through internet, national and international conferences and workshops.

描述(由申请人提供)：为了改进对空气中微生物暴露的评估和控制，并保护处于危险中的种群和资源免受空气中微生物制剂的影响，需要先进的生物气溶胶检测和传感系统。常用的生物气溶胶采样器通常测量活的生物气溶胶，这些生物气溶胶只占空气中微生物菌群的一小部分。在这项研究中，响应PA-04-030“NIOSH探索性发展资助计划(R21)”，我们建议设计、建造和评估一种新型的生物气溶胶采样器，该采样器将提高我们确定空气中总的(活的和不活的)微生物暴露的能力。拟议的采样器将紧凑，具有低功耗，最重要的是，将具有非常高的空气样本浓缩率(100万或更高)。目前，很少有其他采样器能够达到如此高的样品浓缩率，这将显著提高我们检测空气中低浓度微生物的能力。在拟议的采样器中，空气中的颗粒物将被静电沉积在超疏水表面(“荷叶”型)上，它们将被微小的(10-50亩L)滚动液滴清除并收集，例如，一种被称为自清洁的现象。然后，含有被去除的微生物的液滴将被用于现代微生物技术的分析，例如实时聚合酶链式反应(RT-PCR)。新采样器在采集测试细菌和真菌时的整体性能(样品采集、提取及其随后的RT-PCR分析)将在实验室和现场环境中进行评估，并与其他采样器进行比较。具有超疏水收集表面的静电收集器的开发将提高我们确定住宅、职业和环境环境中空气中微生物暴露的能力，从而提高我们保护危险人群的能力。少量采集液的使用将使取样器与各种“芯片实验室”兼容，并可能导致对空气中微生物污染物的近乎实时的测定。此外，使用静电技术作为微粒去除机制，可以收集和检测空气传播的病毒，如SARS或猴痘。这项研究的研究结果和实际建议将通过互联网、国家和国际会议和研讨会向利益攸关方传播。