Genome Editing Test Section

基因组编辑测试部分

基本信息

批准号：
10650354
负责人：
JONATHAN A GREEN
金额：
$ 104.45万
依托单位：
UNIVERSITY OF MISSOURI-COLUMBIA
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-06-01 至 2024-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10650354
关键词：
Address Affect Amino Acid Substitution Animal Model Antibodies Bladder Chemicals Cochlea Collaborations Ensure Epigenetic Process Evaluation Family suidae Gene Delivery Genes Genetic Recombination Genome Goals Individual Inhalation Injections Innate Immune Response Intramuscular Lipids Messenger RNA Methods Modeling Modification Nature Outcome Pathway interactions Physiological Procedures Process Production Proteome Reaction Reagent Reporter Reporting Research Resources Risk Role Route Safety Services Sinus Site Spinal System Technology Testing Therapeutic Time Tissues Toxic effect Translations United States National Institutes of Health Validation Vertebral column Viral Viral Vector Virus adaptive immune response base comparative design efficacy testing experience genome editing genome integrity hazard in vivo evaluation intraperitoneal member nanoparticle neoantigens new technology novel nuclease particle programs response safety testing somatic cell gene editing subcutaneous success tool transcriptome transgene expression vector viral gene delivery

项目摘要

Project Summary -Genome Editing Testing Section The role of the Genome Editing Testing Section will be to validate unique reporter models created by the Animal Model Production Section and to test novel delivery methods and reagents provided by other members of the Somatic Cell Genome Editing (SCGE) Consortium. Additionally, the Genome Editing Testing Section will be responsible for creating detailed standard operating procedures (SOPs) and comprehensive reports for testing new genome editing technologies. It is essential to have a well- established pipeline for the validation and testing of new genome editing technology to facilitate proper refinement of the tools while ensuring the safety of the technology. The Genome Editing Testing Section will streamline the testing process while maintaining a comprehensive and rigorous comparative analysis of new genome editing tools and delivery systems. Our group is ideally suited to provide this testing due to our unique composition of individuals that are experienced with pig production, swine biomedical models, operate successful labs that routinely test cutting-edge genome editing technology, and are associated with world-renowned National Swine Resource and Research Center (NSRRC). The Genome Editing Testing Section will have a significant impact on the overall MU Swine Testing Center for Genome Editing Technologies’ ability to accurately assess new technologies and further facilitate the ability to use genome editing in therapeutic circumstances.

项目摘要 - 基因组编辑测试部分基因组编辑测试部分的作用将是验证由动物模型生产部分，并测试其他提供的新型输送方法和试剂体细胞基因组编辑（SCGE）联盟的成员。此外，基因组编辑测试部分将负责创建详细的标准操作程序（SOP）和测试新基因组编辑技术的综合报告。拥有一个很好的建立的验证和测试新基因组编辑技术的管道以促进适当在确保技术安全性的同时，对工具进行了改进。基因组编辑测试部分将简化测试过程，同时维护全面，严格的比较分析新的基因组编辑工具和输送系统。由于我们的猪生产，猪生物医学模型经验丰富的个体的独特组成，经常经常测试尖端基因组编辑技术的成功实验室，并与世界知名的国家猪资源和研究中心（NSRRC）。基因组编辑测试部分将对基因组编辑的整体MU猪测试中心产生重大影响技术能够准确评估新技术并进一步促进使用基因组的能力在治疗情况下进行编辑。