Development of a novel multipurpose model to propagate and study the tick transmission cycle of relapsing fever spirochetes from Eurasia.
开发一种新型多用途模型,用于繁殖和研究欧亚大陆回归热螺旋体的蜱传播周期。
基本信息
- 批准号:10651550
- 负责人:
- 金额:$ 20.06万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2023
- 资助国家:美国
- 起止时间:2023-07-05 至 2025-06-30
- 项目状态:未结题
- 来源:
- 关键词:AddressAsiaBacteriaBacterial GenomeBloodBorreliaChildChromosomesClimateComparative Genomic AnalysisComplexCountryDataDevelopmentDiseaseGene RearrangementGenesGenetic RecombinationGenomeGenome StabilityGenomicsGoalsGrowthHealthIn VitroKnowledgeLife Cycle StagesLiteratureLongevityMaintenanceMammalsMiddle EastModelingMolecularMolecular ProbesMorbidity - disease rateNatural SourceOrder SpirochaetalesOrnithodorosPathogenesisPhylogenetic AnalysisPlasmidsPoliticsPositioning AttributePublic HealthPublicationsPublishingRelapsing FeverRepliconReportingResearchResource-limited settingResourcesSouthern BlottingSystemTestingTicksTimeUkrainechicken eggcost effectiveeggepidemiology studygenome annotationgenome resourcegenomic toolsimprovedlow income countrymodel developmentmortalityneglectnovelpathogenpathogen genomicspoor communitiesrelapsing fever borreliatick bitetick transmissiontransmission processvectorvector competencevector tickvector-borne pathogen
项目摘要
Project Summary
Relapsing fever (RF) spirochetes are a group of neglected vector-borne pathogens that are a significant cause
of child morbidity and mortality. The current approaches to grow and assess tick transmission of RF spirochetes
are impractical in resource limited settings where they are prevalent. For example, propagating spirochetes in
culture medium is expensive and labor intensive. Moreover, prolonged in vitro propagation in medium causes
DNA rearrangements of their already exceedingly complex genomes, and this results in diminished vector
colonization and pathogenesis. There is a critical need for cost effective practical approaches to grow and study
RF spirochetes from neglected regions of the globe. This project builds on our prior our publication reporting the
first isolation of Borrelia caucasica, which is part of the Persica species complex and is distributed through
Eurasia and the Middle East. Moreover, our recent advances RF spirochete genomics addressed a major
limitation in the field and resulted in complete chromosome and plasmid assembled and annotated genomes.
The genomics tools we developed can now be utilized to characterize a model for the Persica complex species.
Interestingly, historical literature suggested that embryonated chicken eggs (ECEs) could be infected with RF
spirochetes by tick bite and the bacteria grew in this system. However, at the time molecular and genomics
resources were not available to characterize this model and knowledge gaps remain about the pathogens
genomic stability in ECEs. Leveraging recent advances in RF spirochete genomics, the ECE model can now be
fully developed to study Persica complex species. In this study, two aims are proposed. The first aim is to utilize
genomics resources for RF spirochetes and evaluate plasmid diversity and identify unique plasmid markers of
B. caucasica isolates through a comparative genomic analysis. The first objective is to complete reference quality
genomes assemblies of B. caucasica isolates and perform a comparative genomics analysis to identify plasmid
markers. The second objective is to develop a panel of specific probes needed to evaluate plasmid retention and
rearrangements during the B. caucasica life cycle. In the second aim, we will assess genomic changes of B.
caucasica using the tick – ECE model. The first objective is to develop the B. caucasica model in ECEs and
evaluate spirochete growth, tick acquisition, and subsequent tick transmission of spirochetes to naïve eggs. The
second objective is to assess plasmid stability and the ability of B. caucasica to remain infectious after passage
through ECEs. The establishment of the ECE model will result in a cost-effective approach to amplify RF
spirochetes in the absence of culture medium. Moreover, the development of this model offers a practical system
to study the transmission cycle and vector competence of RF spirochetes in resource limited settings. With the
emergence and health burden of RF spirochetes in low-income countries, it is important to develop systems to
propagate and study these neglected pathogens.
项目总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Job E Lopez其他文献
Job E Lopez的其他文献
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{{ truncateString('Job E Lopez', 18)}}的其他基金
Defining the ecology of tick-borne relapsing fever Borrelia turicatae in Austin, Texas.
定义德克萨斯州奥斯汀蜱传回归热疏螺旋体的生态学。
- 批准号:
9895076 - 财政年份:2020
- 资助金额:
$ 20.06万 - 项目类别:
Identification of essential factors contributing to vector colonization and transmission of relapsing fever spirochetes
回归热螺旋体媒介定植和传播的重要因素的鉴定
- 批准号:
10347317 - 财政年份:2018
- 资助金额:
$ 20.06万 - 项目类别:
Identification of essential factors contributing to vector colonization and transmission of relapsing fever spirochetes
回归热螺旋体媒介定植和传播的重要因素的鉴定
- 批准号:
10112814 - 财政年份:2018
- 资助金额:
$ 20.06万 - 项目类别:
Relapsing Fever Spirochete Protein Production within the Vector
载体内回归热螺旋体蛋白的产生
- 批准号:
8908201 - 财政年份:2013
- 资助金额:
$ 20.06万 - 项目类别:
Relapsing fever spirochete protein production within the vector
回归热螺旋体蛋白在载体内的产生
- 批准号:
8583219 - 财政年份:2013
- 资助金额:
$ 20.06万 - 项目类别:
Identifying Borrelia turicatae genes needed for colonization and transmission fro
鉴定Borrelia turicatae定植和传播所需的基因
- 批准号:
8418749 - 财政年份:2012
- 资助金额:
$ 20.06万 - 项目类别:
Identifying Borrelia turicatae genes needed for colonization and transmission fro
鉴定Borrelia turicatae定植和传播所需的基因
- 批准号:
8188983 - 财政年份:2012
- 资助金额:
$ 20.06万 - 项目类别:
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