Structural Biology Core
结构生物学核心
基本信息
- 批准号:10650875
- 负责人:
- 金额:$ 102.28万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-06-22 至 2027-03-31
- 项目状态:未结题
- 来源:
- 关键词:3-DimensionalAchievementAddressAffectAmino AcidsArchitectureBehaviorBindingBiologicalBiologyChemicalsCollaborationsCollectionCommunitiesComplexConsultationsCoupledCryo-electron tomographyCryoelectron MicroscopyDataData CollectionDatabasesDeuteriumDevelopmentEducational workshopEnvironmentEvaluationExperimental DesignsFluorineFutureGoalsHIVHIV-1HydrogenIndividualLaboratoriesLigandsMacromolecular ComplexesMass Spectrum AnalysisMeasurementMethodsMicroscopyMolecularMolecular ConformationMolecular Mechanisms of ActionMolecular ProbesMolecular StructureNMR SpectroscopyNegative StainingNuclear Magnetic ResonanceNucleic AcidsOperating RoomsPhasePlayProteinsRegulationResearchResearch PersonnelResolutionResourcesRoleSamplingServicesStructureTechniquesTechnologyTimeTitanTrainingUnited States National Institutes of HealthValidationViralViral ProteinsVirusVirus Replicationantiretroviral therapycrosslinkdesignimage processinginhibitor therapyinsightinstrumentationinterestmacromolecular assemblymacromoleculemembernew technologynovelparticleprotein complexprotein functionsmall moleculestructural biologysynergismtandem mass spectrometrytechnology developmenttoolvideoconferencevoltage
项目摘要
ABSTRACT, CORE 2
Deciphering the molecular mechanisms underlying key steps of the HIV-1 replication cycle is essential to fully
describe the biology of the virus, its interplay with host components, and for developing novel antiretroviral
therapies. Toward this goal, structural biology plays an important role, as an atomic-level understanding of
macromolecular structure and dynamics, and how alterations in structure affect function, provides mechanistic
insights into the workings of biological macromolecules. The structural biology core (Core 2) will contribute
complementary expertise, instrumentation, and resources for studying key steps of the viral replication cycle, as
described within individual projects, including viral entry/uncoating, integration, and maturation/assembly.
Core 2 includes: hydrogen deuterium exchange coupled to mass spectrometry (HDX-MS) and cross-linking mass
spectrometry (XL-MS), nuclear magnetic resonance (NMR) spectroscopy, single-particle cryo-electron
microscopy (cryo-EM), and cryo-electron tomography (cryo-ET). HDX-MS and XL-MS methods have emerged
as powerful tools to probe protein interactions with ligands, co-regulatory proteins, and nucleic acids in solution
to yield insights into macromolecular behavior on a residue-by-residue level. NMR spectroscopy enables
determining high-resolution structures of small-to-medium size proteins and nucleic acids, probing
macromolecular interactions at atomic resolution and studying conformational dynamics. Single-particle cryo-
EM yields an understanding of macromolecular structure and dynamics for large assemblies, often with less
material and more rapidly than with traditional methods. Cryo-ET and sub-tomogram averaging (STA) is ideally
suited to studying irregular objects such as non-icosahedral protein lattices, where having 3D volumetric
information for each unique assembly, prior to ensemble averaging, is necessary to gain insight into local and
long-range structural perturbations.
Core 2 will apply existing tools to address specific questions described within individual Projects, and each
laboratory will also develop novel technologies to meet the needs of proposed research goals. The Core has
established collaborations with most current B-HIVE investigators, has developed workflows for new projects
that may emerge during the studies, and will provide ongoing consultation and training to B-HIVE members as
projects develop. The established tools provide complementary and synergistic expertise toward the broad
research goals of the center, while the training and dissemination module aligns with the broader impacts of the
NIH.
摘要,核心2
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Dmitry Lyumkis其他文献
Dmitry Lyumkis的其他文献
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{{ truncateString('Dmitry Lyumkis', 18)}}的其他基金
Structural basis for activity of and resistance to HIV integrase inhibitors
HIV整合酶抑制剂的活性和耐药性的结构基础
- 批准号:
9753903 - 财政年份:2017
- 资助金额:
$ 102.28万 - 项目类别:
Structural basis for activity of and resistance to HIV integrase inhibitors
HIV整合酶抑制剂的活性和耐药性的结构基础
- 批准号:
10551720 - 财政年份:2017
- 资助金额:
$ 102.28万 - 项目类别:
Structural basis for activity of and resistance to HIV integrase inhibitors
HIV整合酶抑制剂的活性和耐药性的结构基础
- 批准号:
10238819 - 财政年份:2017
- 资助金额:
$ 102.28万 - 项目类别:
Structural basis for activity of and resistance to HIV integrase inhibitors
HIV整合酶抑制剂的活性和耐药性的结构基础
- 批准号:
10661078 - 财政年份:2017
- 资助金额:
$ 102.28万 - 项目类别:
Breaking Barriers in Structural Biology: Novel CryoEM Methods and Applications
打破结构生物学的障碍:新颖的冷冻电镜方法和应用
- 批准号:
9002750 - 财政年份:2015
- 资助金额:
$ 102.28万 - 项目类别:
Breaking Barriers in Structural Biology: Novel CryoEM Methods and Applications
打破结构生物学的障碍:新颖的冷冻电镜方法和应用
- 批准号:
9349372 - 财政年份:2015
- 资助金额:
$ 102.28万 - 项目类别:
Breaking Barriers in Structural Biology: Novel CryoEM Methods and Applications
打破结构生物学的障碍:新颖的冷冻电镜方法和应用
- 批准号:
9561928 - 财政年份:2015
- 资助金额:
$ 102.28万 - 项目类别:
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