Imaging Structure and Function of Photoreceptors

项目总结/摘要 人类的视觉始于光感受器收集并对光线做出反应。正常的感光功能是 这是正常视力所必需的，但在体内评估这些过程的技术有限。新的光学模式 这些快速、特异和非侵入性的方法有望大大扩展我们更准确监测的能力， 和完全的光感受器 这项研究利用了我实验室开发的独特的自适应光学OCT仪器， 结合用于亚细胞图像配准和相敏检测的自定义算法， 测量单个光感受器的解剖学和生理学特性。我们将利用这项技术， 研究了三个具体的目标，量化光感受器的光谱敏感性曲线，表达 色觉缺陷中的光感受器光谱类型，以及色觉缺陷中光感受器损失的时间动态。 视网膜色素变性患者。本研究的长期目标是建立高分辨率、高特异性的 光学技术作为探测视网膜细胞结构和生理过程的有效工具 规模由此产生的在体内研究细胞的能力将改善对以下疾病的早期检测和治疗监测： 影响视网膜的疾病。

Project Summary/Abstract Human vision starts when photoreceptors collect and respond to light. Normal photoreceptor function is essential for normal vision, yet techniques to assess these processes in vivo are limited. New optical modalities that are rapid, specific, and non‐invasive promise to greatly expand our capability to monitor more accurately and completely photoreceptors. This study takes advantage of unique adaptive‐optics OCT instrumentation developed in my laboratory in conjunction with custom algorithms for sub‐cellular image registration and phase‐sensitive detection to measure anatomical and physiological properties of individual photoreceptors. We will use this technique to investigate three specific aims that quantify the spectral sensitivity profiles of photoreceptors, the expression of photoreceptor spectral types in color vision deficiencies, and the temporal dynamics of photoreceptor loss in retinitis pigmentosa patients. The long term goal of this research is to establish high resolution, high specificity optical techniques as valid tools for probing structure and physiologic processes of the retina at the cellular scale. The resulting ability to study cells in vivo will improve early detection of and treatment monitoring for diseases that impact the retina.