Imaging Structure and Function of Photoreceptors

项目摘要/摘要 当光感受器收集光并对光做出反应时，人类的视觉就开始了。正常的光感受器功能是 对于正常的视力来说是必不可少的，然而在体内评估这些过程的技术是有限的。新的光学模式 这是快速、具体和非侵入性的承诺，可以极大地扩展我们更准确地监控的能力 和完全的光感受器。 这项研究利用了我的实验室在#年开发的独特的自适应光学OCT仪器 结合用于亚细胞图像配准和相敏检测的自定义算法 测量单个光感受器的解剖和生理特性。我们将使用此技术来 研究量化光感受器光谱敏感性分布的三个特定目标，表达 色觉缺陷的光感受器光谱类型和光感受器丢失的时间动力学 视网膜色素变性患者。这项研究的长期目标是建立高分辨率、高特异性 光学技术是探测视网膜细胞结构和生理过程的有效工具 比例。由此产生的在体内研究细胞的能力将改善早期发现和治疗监测 影响视网膜的疾病。

Project Summary/Abstract Human vision starts when photoreceptors collect and respond to light. Normal photoreceptor function is essential for normal vision, yet techniques to assess these processes in vivo are limited. New optical modalities that are rapid, specific, and non‐invasive promise to greatly expand our capability to monitor more accurately and completely photoreceptors. This study takes advantage of unique adaptive‐optics OCT instrumentation developed in my laboratory in conjunction with custom algorithms for sub‐cellular image registration and phase‐sensitive detection to measure anatomical and physiological properties of individual photoreceptors. We will use this technique to investigate three specific aims that quantify the spectral sensitivity profiles of photoreceptors, the expression of photoreceptor spectral types in color vision deficiencies, and the temporal dynamics of photoreceptor loss in retinitis pigmentosa patients. The long term goal of this research is to establish high resolution, high specificity optical techniques as valid tools for probing structure and physiologic processes of the retina at the cellular scale. The resulting ability to study cells in vivo will improve early detection of and treatment monitoring for diseases that impact the retina.