Single Cell Genomics Core

核心C：单细胞基因组学(SCG)核心 摘要 现在高通量的基因组、蛋白质组、代谢和免疫图谱技术 提供丰富的数据，可以挖掘这些数据来询问基本的生物过程、变化 细胞或生物种群，以及疾病的分子基础，从 从整个有机体到单个细胞。此类数据的生成和分析现在是 生命科学中的研究方法和问题。数量生物学中心 (CQB)将首先形成核，然后成长为这种湿板凳上的“组学研究”与 达特茅斯大学的生物信息学。单细胞基因组学(SCG)已成为 了解涉及复杂生物系统的疾病，包括癌症、神经退行性变 免疫/自身免疫性疾病。SCG核心的总体目标是开发一个集中的 CQB内部的资源，用于支持个别项目负责人和达特茅斯社区。这个 这个核心的目标是实施、维护和改进所需的技术，以进行 SCG以严格和质量控制的方式进行实验。SCG核心将直接工作 在Cobre的领导下，项目负责人、导师和研究人员一起设计强大的实验 并将实施严格的质量控制和样品追踪方法(目标1)，以确保 生成高质量、可重现的数据。SCG核心还将维护设备和 能够分离、捕获、文库制备和测序DNA和RNA的专业知识 科布雷研究人员和整个达特茅斯研究界的单一牢房(目标2)。 最后，与分析核心(核心B)合作，SCG核心将提供 用于分析和可视化这些单细胞数据的计算基础设施和专业知识， 加快从实验设计到生物学洞察的进程(目标3)。SCG核心将 与时俱进，作为专业知识和技术的中心 全校的调查人员。

Core C: Single Cell Genomics (SCG) Core ABSTRACT High-throughput genomic, proteomic, metabolomic, and immune profiling technologies now provide a wealth of data that can be mined to interrogate basic biological process, changes in cellular or organismal populations, and the molecular bases of disease, at levels ranging from the whole organism to single cells. The generation and analysis of such data is now a pillar of research approaches and problems in the life sciences. The Center for Quantitative Biology (CQB) will first nucleate and then grow this fusion of wet bench “omics research” with bioinformatics at Dartmouth. Single Cell Genomics (SCG) has become an essential tool to understand diseases involving complex biological systems including cancer, neurodegenerative and immune/autoimmune diseases. The overall goal of the SCG core is to develop a centralized resource within the CQB to support individual project leaders and the Dartmouth community. The goal of this core is to implement, maintain and improve upon the technologies required to conduct SCG experiments in a rigorous and quality controlled manner. The SCG Core will work directly with COBRE leadership, project leaders, mentors and investigators to design robust experiments and will implement stringent quality control and sample tracking methodologies (Aim 1) to ensure generation of high quality, reproducible data. The SCG core will also maintain equipment and expertise to enable isolation, capture, library preparation and sequencing of DNA and RNA from single cells for COBRE investigators and the Dartmouth research community at large (Aim 2). Finally, in collaboration with the Analytics Core (Core B), the SCG Core will provide the computational infrastructure and expertise to analyze and visualize these single cell data, expediting the process from experimental design to biological insight (Aim 3). The SCG core will stay abreast of advancements in the field, serving as a hub of expertise and technology to investigators campus-wide.