Single Cell Genomics Core

单细胞基因组核心

• 批准号: 10663283
• 负责人: MICHAEL L WHITFIELD
• 金额: $ 31.87万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10663283
• 关键词: Antibiotic Resistance; Autoimmune Diseases; Autoimmunity; Bar Codes; Bioinformatics; Biological; Biological Process; Biological Sciences; Biology; Cancer Biology; Cell Separation; Cells; Centers of Research Excellence; Chemistry; Chromium; Chronobiology; Clinical; Collaborations; Communication; Communities; Computer software; Consultations; Cutaneous Melanoma; DNA sequencing; Data; Data Analyses; Databases; Development; Discipline; Disease; Dissociation; Educational process of instructing; Educational workshop; Ensure; Equipment; Event; Experimental Designs; Foundations; Funding; Gene Expression; Generations; Genomics; Goals; Grant; Health; Heterogeneity; Human Resources; Immune; Immune response; Immune system; Immunology; Immunotherapy; Individual; International; Internet; Laboratories; Leadership; Libraries; Link; Malignant Neoplasms; Management Information Systems; Mentors; Methodology; Methods; Molecular; Nerve Degeneration; Nucleic Acids; Organism; Patients; Phenotype; Population; Preparation; Privatization; Procedures; Process; Proteomics; Protocols documentation; Publishing; Quality Control; RNA; Reproducibility; Research; Research Personnel; Resistance; Resources; Sampling; Services; Skin; Standardization; System; Systemic Scleroderma; Technology; Time; Tissues; Translating; United States National Institutes of Health; Videoconferencing; Visualization; Whole Organism; Work; base; body system; cell type; clinically relevant; complex biological systems; computer infrastructure; data analysis pipeline; data pipeline; data quality; design; digital; epigenome; experience; experimental study; improved; innovation; insight; machine learning model; mathematical model; meetings; metabolomics; outcome prediction; protocol development; single-cell RNA sequencing; success; therapy resistant; tool; transcriptome sequencing; tumor; tumor progression

Core C: Single Cell Genomics (SCG) Core ABSTRACT High-throughput genomic, proteomic, metabolomic, and immune profiling technologies now provide a wealth of data that can be mined to interrogate basic biological process, changes in cellular or organismal populations, and the molecular bases of disease, at levels ranging from the whole organism to single cells. The generation and analysis of such data is now a pillar of research approaches and problems in the life sciences. The Center for Quantitative Biology (CQB) will first nucleate and then grow this fusion of wet bench “omics research” with bioinformatics at Dartmouth. Single Cell Genomics (SCG) has become an essential tool to understand diseases involving complex biological systems including cancer, neurodegenerative and immune/autoimmune diseases. The overall goal of the SCG core is to develop a centralized resource within the CQB to support individual project leaders and the Dartmouth community. The goal of this core is to implement, maintain and improve upon the technologies required to conduct SCG experiments in a rigorous and quality controlled manner. The SCG Core will work directly with COBRE leadership, project leaders, mentors and investigators to design robust experiments and will implement stringent quality control and sample tracking methodologies (Aim 1) to ensure generation of high quality, reproducible data. The SCG core will also maintain equipment and expertise to enable isolation, capture, library preparation and sequencing of DNA and RNA from single cells for COBRE investigators and the Dartmouth research community at large (Aim 2). Finally, in collaboration with the Analytics Core (Core B), the SCG Core will provide the computational infrastructure and expertise to analyze and visualize these single cell data, expediting the process from experimental design to biological insight (Aim 3). The SCG core will stay abreast of advancements in the field, serving as a hub of expertise and technology to investigators campus-wide.

核心 C：单细胞基因组学 (SCG) 核心 抽象的 现在的高通量基因组、蛋白质组、代谢组和免疫分析技术 提供丰富的数据，可以挖掘这些数据来询问基本的生物过程、变化 细胞或有机体群体，以及疾病的分子基础，其水平范围从 整个有机体到单个细胞。此类数据的生成和分析现在已成为 生命科学研究方法和问题。定量生物学中心 （CQB）将首先将湿台“组学研究”与 达特茅斯生物信息学。单细胞基因组学 (SCG) 已成为重要工具 了解涉及复杂生物系统的疾病，包括癌症、神经退行性疾病 和免疫/自身免疫性疾病。 SCG核心的总体目标是开发一个中心化的 CQB 内的资源，用于支持个人项目领导者和达特茅斯社区。这 该核心的目标是实施、维护和改进进行 SCG 以严格且质量受控的方式进行实验。 SCG核心将直接工作 与 COBRE 领导层、项目负责人、导师和研究人员一起设计稳健的实验 并将实施严格的质量控制和样品跟踪方法（目标 1），以确保 生成高质量、可重复的数据。 SCG核心还将维护设备和 专业知识，可实现 DNA 和 RNA 的分离、捕获、文库制备和测序 为 COBRE 研究人员和整个达特茅斯研究界提供单细胞（目标 2）。 最后，SCG 核心将与分析核心（核心 B）合作，提供 用于分析和可视化这些单细胞数据的计算基础设施和专业知识， 加快从实验设计到生物学洞察的过程（目标 3）。 SCG 核心将 紧跟该领域的进步，作为专业知识和技术的中心 调查员遍布校园。