Single Cell Genomics Core
单细胞基因组核心
基本信息
- 批准号:10663283
- 负责人:
- 金额:$ 31.87万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2019
- 资助国家:美国
- 起止时间:2019-08-01 至 2024-06-30
- 项目状态:已结题
- 来源:
- 关键词:Antibiotic ResistanceAutoimmune DiseasesAutoimmunityBar CodesBioinformaticsBiologicalBiological ProcessBiological SciencesBiologyCancer BiologyCell SeparationCellsCenters of Research ExcellenceChemistryChromiumChronobiologyClinicalCollaborationsCommunicationCommunitiesComputer softwareConsultationsCutaneous MelanomaDNA sequencingDataData AnalysesDatabasesDevelopmentDisciplineDiseaseDissociationEducational process of instructingEducational workshopEnsureEquipmentEventExperimental DesignsFoundationsFundingGene ExpressionGenerationsGenomicsGoalsGrantHealthHeterogeneityHuman ResourcesImmuneImmune responseImmune systemImmunologyImmunotherapyIndividualInternationalInternetLaboratoriesLeadershipLibrariesLinkMalignant NeoplasmsManagement Information SystemsMentorsMethodologyMethodsMolecularNerve DegenerationNucleic AcidsOrganismPatientsPhenotypePopulationPreparationPrivatizationProceduresProcessProteomicsProtocols documentationPublishingQuality ControlRNAReproducibilityResearchResearch PersonnelResistanceResourcesSamplingServicesSkinStandardizationSystemSystemic SclerodermaTechnologyTimeTissuesTranslatingUnited States National Institutes of HealthVideoconferencingVisualizationWhole OrganismWorkbasebody systemcell typeclinically relevantcomplex biological systemscomputer infrastructuredata analysis pipelinedata pipelinedata qualitydesigndigitalepigenomeexperienceexperimental studyimprovedinnovationinsightmachine learning modelmathematical modelmeetingsmetabolomicsoutcome predictionprotocol developmentsingle-cell RNA sequencingsuccesstherapy resistanttooltranscriptome sequencingtumortumor progression
项目摘要
Core C: Single Cell Genomics (SCG) Core
ABSTRACT
High-throughput genomic, proteomic, metabolomic, and immune profiling technologies now
provide a wealth of data that can be mined to interrogate basic biological process, changes in
cellular or organismal populations, and the molecular bases of disease, at levels ranging from the
whole organism to single cells. The generation and analysis of such data is now a pillar of
research approaches and problems in the life sciences. The Center for Quantitative Biology
(CQB) will first nucleate and then grow this fusion of wet bench “omics research” with
bioinformatics at Dartmouth. Single Cell Genomics (SCG) has become an essential tool to
understand diseases involving complex biological systems including cancer, neurodegenerative
and immune/autoimmune diseases. The overall goal of the SCG core is to develop a centralized
resource within the CQB to support individual project leaders and the Dartmouth community. The
goal of this core is to implement, maintain and improve upon the technologies required to conduct
SCG experiments in a rigorous and quality controlled manner. The SCG Core will work directly
with COBRE leadership, project leaders, mentors and investigators to design robust experiments
and will implement stringent quality control and sample tracking methodologies (Aim 1) to ensure
generation of high quality, reproducible data. The SCG core will also maintain equipment and
expertise to enable isolation, capture, library preparation and sequencing of DNA and RNA from
single cells for COBRE investigators and the Dartmouth research community at large (Aim 2).
Finally, in collaboration with the Analytics Core (Core B), the SCG Core will provide the
computational infrastructure and expertise to analyze and visualize these single cell data,
expediting the process from experimental design to biological insight (Aim 3). The SCG core will
stay abreast of advancements in the field, serving as a hub of expertise and technology to
investigators campus-wide.
核心C:单细胞基因组学(SCG)核心
摘要
高通量基因组学、蛋白质组学、代谢组学和免疫分析技术现在
提供了丰富的数据,可以挖掘询问基本的生物过程,
细胞或有机体群体,以及疾病的分子基础,水平从
整个生物体到单个细胞。这些数据的生成和分析现在是
生命科学的研究方法和问题。定量生物学中心
(CQB)将首先形成核,然后发展这种融合湿台“组学研究”与
达特茅斯的生物信息学单细胞基因组学(SCG)已成为一种重要的工具,
了解涉及复杂生物系统的疾病,包括癌症,神经退行性疾病,
和免疫/自身免疫性疾病。SCG核心的总体目标是开发一个集中的
CQB内部的资源,以支持个人项目负责人和达特茅斯社区。的
该核心的目标是实施、维护和改进执行所需的技术
SCG实验以严格和质量控制的方式进行。SCG核心将直接
与COBRE领导层、项目负责人、导师和研究人员一起设计强大的实验
并将实施严格的质量控制和样本跟踪方法(目标1),以确保
生成高质量、可再现的数据。SCG核心还将维护设备,
专业知识,使分离,捕获,文库制备和测序的DNA和RNA从
为COBRE研究人员和达特茅斯研究团体提供单细胞(目标2)。
最后,SCG核心将与分析核心(核心B)合作,提供
计算基础设施和专业知识来分析和可视化这些单细胞数据,
加快从实验设计到生物学洞察的过程(目标3)。SCG核心将
与该领域的进步保持同步,作为专业知识和技术的中心,
全校范围内的调查员
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
MICHAEL L WHITFIELD其他文献
MICHAEL L WHITFIELD的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('MICHAEL L WHITFIELD', 18)}}的其他基金
Center for Quantitative Biology Administrative Core
定量生物学中心行政核心
- 批准号:
10434070 - 财政年份:2019
- 资助金额:
$ 31.87万 - 项目类别:
Center for Quantitative Biology: A focus on "omics", from organisms to single cells Supplement 2
定量生物学中心:关注“组学”,从有机体到单细胞补充2
- 批准号:
10853928 - 财政年份:2019
- 资助金额:
$ 31.87万 - 项目类别:
Center for Quantitative Biology: A focus on "omics", from organisms to single cells
定量生物学中心:关注“组学”,从有机体到单细胞
- 批准号:
10212411 - 财政年份:2019
- 资助金额:
$ 31.87万 - 项目类别:
Center for Quantitative Biology Administrative Core
定量生物学中心行政核心
- 批准号:
10212412 - 财政年份:2019
- 资助金额:
$ 31.87万 - 项目类别:
Center for Quantitative Biology Administrative Core
定量生物学中心行政核心
- 批准号:
10663279 - 财政年份:2019
- 资助金额:
$ 31.87万 - 项目类别:
SARS-CoV-2 Surveillance Studies and Genome Sequencing in Rural New England
新英格兰农村地区的 SARS-CoV-2 监测研究和基因组测序
- 批准号:
10381159 - 财政年份:2019
- 资助金额:
$ 31.87万 - 项目类别:
Center for Quantitative Biology: A focus on "omics", from organisms to single cells
定量生物学中心:关注“组学”,从有机体到单细胞
- 批准号:
10434069 - 财政年份:2019
- 资助金额:
$ 31.87万 - 项目类别:
Enabling single molecule spatial transcriptomics with the Vizgen MERSCOPE in situ hybridization solution at Dartmouth and beyond
在达特茅斯及其他地区使用 Vizgen MERSCOPE 原位杂交解决方案实现单分子空间转录组学
- 批准号:
10581931 - 财政年份:2019
- 资助金额:
$ 31.87万 - 项目类别:
Center for Quantitative Biology: A focus on "omics", from organisms to single cells
定量生物学中心:关注“组学”,从有机体到单细胞
- 批准号:
10663278 - 财政年份:2019
- 资助金额:
$ 31.87万 - 项目类别:
相似国自然基金
Autoimmune diseases therapies: variations on the microbiome in rheumatoid arthritis
- 批准号:31171277
- 批准年份:2011
- 资助金额:60.0 万元
- 项目类别:面上项目
相似海外基金
Effects of maternal immune activation on autoimmune diseases in offsprings
母体免疫激活对后代自身免疫性疾病的影响
- 批准号:
23H02155 - 财政年份:2023
- 资助金额:
$ 31.87万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Autoantibodies and antibody-secreting cells in neurological autoimmune diseases: from biology to therapy
神经性自身免疫性疾病中的自身抗体和抗体分泌细胞:从生物学到治疗
- 批准号:
479128 - 财政年份:2023
- 资助金额:
$ 31.87万 - 项目类别:
Operating Grants
IPP: AUTOIMMUNE DISEASES STATISTICAL AND CLINICAL COORDINATING CENTER (ADSCCC)
IPP:自身免疫性疾病统计和临床协调中心 (ADSCCC)
- 批准号:
10788032 - 财政年份:2023
- 资助金额:
$ 31.87万 - 项目类别:
Biomarkers of vascular endothelial dysfunction in systemic autoimmune diseases: analysis of circulating microRNAs
系统性自身免疫性疾病中血管内皮功能障碍的生物标志物:循环 microRNA 分析
- 批准号:
23K14742 - 财政年份:2023
- 资助金额:
$ 31.87万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
NOVEL HUMORAL AND CELLULAR BIOMARKERS OF AUTOIMMUNE DISEASES CAUSED BY IMMUNOTHERAPY
免疫治疗引起的自身免疫性疾病的新型体液和细胞生物标志物
- 批准号:
10593224 - 财政年份:2023
- 资助金额:
$ 31.87万 - 项目类别:
Structural mechanisms of autoimmune diseases targeting cys-loop receptors
针对半胱氨酸环受体的自身免疫性疾病的结构机制
- 批准号:
10864719 - 财政年份:2023
- 资助金额:
$ 31.87万 - 项目类别:
Developing non-immunosuppressive immune-based therapeutics for targeted treatment of autoimmune diseases
开发非免疫抑制性免疫疗法来靶向治疗自身免疫性疾病
- 批准号:
10586562 - 财政年份:2023
- 资助金额:
$ 31.87万 - 项目类别:
Regulation of autoimmune diseases by PTPN22 phosphatase
PTPN22磷酸酶对自身免疫性疾病的调节
- 批准号:
23K06589 - 财政年份:2023
- 资助金额:
$ 31.87万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Decipher and target GABA metabolism and GABA receptor-mediated signaling in autoimmune diseases
破译并靶向自身免疫性疾病中的 GABA 代谢和 GABA 受体介导的信号传导
- 批准号:
10623380 - 财政年份:2023
- 资助金额:
$ 31.87万 - 项目类别:
Targeting the long isoform of the prolactin receptor to treat autoimmune diseases and B-cell malignancies
靶向催乳素受体的长亚型来治疗自身免疫性疾病和 B 细胞恶性肿瘤
- 批准号:
10735148 - 财政年份:2023
- 资助金额:
$ 31.87万 - 项目类别: