Synaptic and dendritic physiology in the prefrontal cortex
前额皮质的突触和树突生理学
基本信息
- 批准号:10532203
- 负责人:
- 金额:$ 50.63万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2010
- 资助国家:美国
- 起止时间:2010-01-06 至 2024-11-30
- 项目状态:已结题
- 来源:
- 关键词:AnimalsApicalAreaAttention deficit hyperactivity disorderBehaviorCell NucleusCellsCognitiveComplexDataDendritesDistalElectrophysiology (science)Functional disorderGoalsImageInterneuronsMediatingMental disordersMusPhotonsPhysiologyPlayPopulationPrefrontal CortexPrimatesPropertyPyramidal CellsRodentRoleSchizophreniaShapesSignal TransductionSynapsesTestingThalamic NucleiThalamic structureTransgenic MiceWhole-Cell RecordingsWorkcell typecognitive functionexperimental studyinsightneuronal cell bodyneuropsychiatric disordernoveloptogeneticssensory cortexsensory systemtherapeutic targettwo photon microscopytwo-photon
项目摘要
PROJECT SUMMARY
Interactions between the thalamus and prefrontal cortex (PFC) are important for cognitive function in animals
ranging from rodents to primates. The importance of these long-range networks is highlighted by multiple
neuropsychiatric diseases, including schizophrenia and ADHD. However, most of what we know about
thalamo-cortical circuits comes from sensory systems, where primary thalamic inputs arrive in layer 4 (L4). In
contrast, the mouse PFC is an agranular area that lacks L4, and instead receives higher-order thalamic inputs
directly to superficial layers. We recently discovered that the PFC makes reciprocal connections with both the
mediodorsal (MD) and ventromedial (VM) thalamus. These thalamic nuclei support distinct behaviors, but the
cellular, synaptic and circuit mechanisms for their interactions with PFC are poorly understood. We found MD
strongly drives layer 2/3 (L2/3) pyramidal cells, whereas VM inputs contact the dendrites of a subset of L5
pyramidal cells. Interestingly, both inputs also robustly engage inhibitory networks to drive local inhibition
mediated by GABAergic interneurons. The goal of this proposal is to assess how thalamic inputs engage
different populations of superficial interneurons to mediate inhibition in the PFC. In Specific Aim 1, we use
optogenetics and electrophysiology to study how thalamic inputs drive multiple classes of interneurons in
superficial layers. Our preliminary data suggests that MD and VM engage complementary populations of
interneurons located in different sub-layers. In Specific Aim 2, we then use conditional optogenetics to study
how these interneurons contact excitatory and inhibitory cells within and across layers. Our preliminary data
indicate that the interneurons contacted by MD and VM participate in distinct inhibitory and disinhibitory circuits
across multiple layers. Lastly, in Specific Aim 3, we combine 1-photon optogenetics with 2-photon microscopy
to study how specific populations of interneurons mediate the suppression of dendritic Ca2+ spikes. Our
preliminary data reveal that a sub-population of superficial interneurons mediates robust feed-forward inhibition
in the dendrites. Together, the results from our experiments will answer fundamental questions about the
organization of thalamo-cortical circuits and interneurons in the PFC. They will also help identify potential
therapeutic targets for the many neuropsychiatric disorders that arise from disrupted circuitry within the PFC.
项目总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Adam G Carter其他文献
Adam G Carter的其他文献
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{{ truncateString('Adam G Carter', 18)}}的其他基金
Thalamus in the middle: computations in multi-regional neural circuits
中间的丘脑:多区域神经回路的计算
- 批准号:
10546504 - 财政年份:2022
- 资助金额:
$ 50.63万 - 项目类别:
Impact of subcortical inputs on frontal cortex via thalamus
皮层下输入通过丘脑对额叶皮层的影响
- 批准号:
10294403 - 财政年份:2022
- 资助金额:
$ 50.63万 - 项目类别:
Thalamus in the middle: computations in multi-regional neural circuits
中间的丘脑:多区域神经回路的计算
- 批准号:
10294397 - 财政年份:2022
- 资助金额:
$ 50.63万 - 项目类别:
Impact of subcortical inputs on frontal cortex via thalamus
皮层下输入通过丘脑对额叶皮层的影响
- 批准号:
10546514 - 财政年份:2022
- 资助金额:
$ 50.63万 - 项目类别:
The impact of drugs of abuse on striatal circuits
滥用药物对纹状体回路的影响
- 批准号:
8767134 - 财政年份:2014
- 资助金额:
$ 50.63万 - 项目类别:
The impact of drugs of abuse on striatal circuits
滥用药物对纹状体回路的影响
- 批准号:
9068059 - 财政年份:2014
- 资助金额:
$ 50.63万 - 项目类别:
Dendritic physiology and calcium signaling in pyramidal neurons of the prefrontal
前额叶锥体神经元的树突生理学和钙信号传导
- 批准号:
7783980 - 财政年份:2010
- 资助金额:
$ 50.63万 - 项目类别:
Synaptic and dendritic physiology in the prefrontal cortex
前额皮质的突触和树突生理学
- 批准号:
8990978 - 财政年份:2010
- 资助金额:
$ 50.63万 - 项目类别:
Synaptic and Dendritic Physiology in the Prefrontal Cortex
前额皮质的突触和树突生理学
- 批准号:
8402157 - 财政年份:2010
- 资助金额:
$ 50.63万 - 项目类别:
Dendritic physiology and calcium signaling in pyramidal neurons of the prefrontal
前额叶锥体神经元的树突生理学和钙信号传导
- 批准号:
8206675 - 财政年份:2010
- 资助金额:
$ 50.63万 - 项目类别:
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