Synaptic transmission at retinal ribbon synapses
视网膜带突触的突触传递
基本信息
- 批准号:10544542
- 负责人:
- 金额:$ 46.74万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-01-01 至 2024-12-31
- 项目状态:已结题
- 来源:
- 关键词:Action PotentialsAffectAfferent NeuronsAmacrine CellsAnimal ModelAnimalsAnionsAuditory systemBindingBiological AssayBlindnessCalciumCellsChargeCommunicationCoupledDarknessDataDiseaseDockingElectric CapacitanceElectrophysiology (science)ElectroretinographyExcisionExhibitsExocytosisEyeGeneticGlutamate TransporterGlutamatesGrantHair CellsHearingImageIndividualInheritedInner Hair CellsInvestigationKineticsKnockout MiceLaboratoriesLabyrinthLocationMeasuresMembraneMembrane PotentialsMental DepressionMental HealthModelingMonitorMorphologyMovementMultiprotein ComplexesMusNervous SystemNeuronsNeurotransmittersOrganellesPhotoreceptorsPhysiologicalPhysiologyProcessPropertyProteinsRecoveryRetinaRodRoleSensorySiteSpeedStructureSynapsesSynaptic TransmissionSynaptic VesiclesTestingTransgenic OrganismsTravelUncertaintyVertebrate PhotoreceptorsVesicleVisionVisual SystemZebrafishcell typedeafnesselectrical propertyexperimental studyimaging studyknockout animalmeternervous system disorderneurotransmissionneurotransmitter releasepresynapticpresynaptic neuronsrecruitribbon synapsesensory stimulustransmission processvesicular releasevoltage clamp
项目摘要
In sensory cells of the eye and inner ear the neurotransmitter glutamate is released at active zones in a graded
and continuous manner. These cells have evolved specialized structures known as synaptic ribbons. These
proteinaceous structures tether synaptic vesicles near active zones. Based on their location, abundance of
tethered vesicles, and properties of the sensory neurons in which they are found, these organelles have long
been thought to be important for maintaining the continuous release of glutamate. Several other functions have
also been ascribed to ribbons. However, recent evidence casts doubt on these ideas. The focus of this grant is
to understand the role of synaptic ribbons in sensory synaptic transmission using animal models that lack
Ribeye, a protein that is both the most abundant within the ribbon and not found anywhere else. Lack of Ribeye
leads to loss of membrane associated synaptic ribbons, without loss of other presynaptic proteins. Specific Aim 1
investigates how Ribeye removal and loss of synaptic ribbons affects neurotransmitter release from
photoreceptors. Specific Aim 2 looks at the properties of synaptic release in bipolar cells from the same animals.
In Specific Aim 3, we will image single synaptic vesicles in cells lacking Ribeye to measure rates of vesicle
movement and replenishment to test the role of the ribbon in these processes. Understanding ribbon function
may provide clues to help understand diseases that specifically affect vision and hearing. In addition, the
fundamental understanding of presynaptic processes in these specialized neurons will have broader implications
for neuronal communication in general and thus, may contribute to our understanding of various aspects of
mental health and neurological disorders.
在眼睛和内耳的感觉细胞中,神经递质谷氨酸在活动区以分级的方式释放
和连续不断的态度。这些细胞进化出了称为突触带的特殊结构。这些
蛋白结构系住活动区附近的突触小泡。根据他们的位置,丰富的
系留的小泡,以及发现它们的感觉神经元的特性,这些细胞器有很长的
一直被认为是维持谷氨酸持续释放的重要因素。其他几个函数具有
也被归因于丝带。然而,最近的证据让人对这些想法产生了怀疑。这笔赠款的重点是
使用缺乏突触的动物模型来了解突触带在感觉突触传递中的作用
Ribeye,一种在丝带中含量最丰富的蛋白质,在其他地方找不到。缺少Ribeye
导致膜相关突触带的丢失,而不损失其他突触前蛋白。具体目标1
研究核眼的去除和突触带的丢失如何影响神经递质的释放。
光感受器。特定目标2着眼于同一动物双极细胞中突触释放的特性。
在特定的目标3中,我们将成像缺少Ribeye的细胞中的单个突触小泡来测量小泡的速率
移动和补给,以测试丝带在这些过程中的作用。了解功能区功能
可能会提供线索,帮助理解专门影响视力和听力的疾病。此外,
对这些特殊神经元突触前过程的基本了解将具有更广泛的意义
从而有助于我们理解神经的各个方面。
精神健康和神经紊乱。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
WALLACE B THORESON其他文献
WALLACE B THORESON的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('WALLACE B THORESON', 18)}}的其他基金
Synaptic transmission at retinal ribbon synapses
视网膜带突触的突触传递
- 批准号:
10322145 - 财政年份:2021
- 资助金额:
$ 46.74万 - 项目类别:
Synaptic transmission at retinal ribbon synapses
视网膜带突触的突触传递
- 批准号:
10154012 - 财政年份:2021
- 资助金额:
$ 46.74万 - 项目类别:
CHLORIDE REGULATION OF PHOTORECEPTOR NEUROTRANSMISSION
光感受器神经传递的氯化物调节
- 批准号:
2888443 - 财政年份:1996
- 资助金额:
$ 46.74万 - 项目类别:
CHLORIDE REGULATION OF PHOTORECEPTOR NEUROTRANSMISSION
光感受器神经传递的氯化物调节
- 批准号:
2711106 - 财政年份:1996
- 资助金额:
$ 46.74万 - 项目类别:
相似海外基金
How Does Particle Material Properties Insoluble and Partially Soluble Affect Sensory Perception Of Fat based Products
不溶性和部分可溶的颗粒材料特性如何影响脂肪基产品的感官知觉
- 批准号:
BB/Z514391/1 - 财政年份:2024
- 资助金额:
$ 46.74万 - 项目类别:
Training Grant
BRC-BIO: Establishing Astrangia poculata as a study system to understand how multi-partner symbiotic interactions affect pathogen response in cnidarians
BRC-BIO:建立 Astrangia poculata 作为研究系统,以了解多伙伴共生相互作用如何影响刺胞动物的病原体反应
- 批准号:
2312555 - 财政年份:2024
- 资助金额:
$ 46.74万 - 项目类别:
Standard Grant
RII Track-4:NSF: From the Ground Up to the Air Above Coastal Dunes: How Groundwater and Evaporation Affect the Mechanism of Wind Erosion
RII Track-4:NSF:从地面到沿海沙丘上方的空气:地下水和蒸发如何影响风蚀机制
- 批准号:
2327346 - 财政年份:2024
- 资助金额:
$ 46.74万 - 项目类别:
Standard Grant
Graduating in Austerity: Do Welfare Cuts Affect the Career Path of University Students?
紧缩毕业:福利削减会影响大学生的职业道路吗?
- 批准号:
ES/Z502595/1 - 财政年份:2024
- 资助金额:
$ 46.74万 - 项目类别:
Fellowship
Insecure lives and the policy disconnect: How multiple insecurities affect Levelling Up and what joined-up policy can do to help
不安全的生活和政策脱节:多种不安全因素如何影响升级以及联合政策可以提供哪些帮助
- 批准号:
ES/Z000149/1 - 财政年份:2024
- 资助金额:
$ 46.74万 - 项目类别:
Research Grant
感性個人差指標 Affect-X の構築とビスポークAIサービスの基盤確立
建立个人敏感度指数 Affect-X 并为定制人工智能服务奠定基础
- 批准号:
23K24936 - 财政年份:2024
- 资助金额:
$ 46.74万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
How does metal binding affect the function of proteins targeted by a devastating pathogen of cereal crops?
金属结合如何影响谷类作物毁灭性病原体靶向的蛋白质的功能?
- 批准号:
2901648 - 财政年份:2024
- 资助金额:
$ 46.74万 - 项目类别:
Studentship
Investigating how double-negative T cells affect anti-leukemic and GvHD-inducing activities of conventional T cells
研究双阴性 T 细胞如何影响传统 T 细胞的抗白血病和 GvHD 诱导活性
- 批准号:
488039 - 财政年份:2023
- 资助金额:
$ 46.74万 - 项目类别:
Operating Grants
New Tendencies of French Film Theory: Representation, Body, Affect
法国电影理论新动向:再现、身体、情感
- 批准号:
23K00129 - 财政年份:2023
- 资助金额:
$ 46.74万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The Protruding Void: Mystical Affect in Samuel Beckett's Prose
突出的虚空:塞缪尔·贝克特散文中的神秘影响
- 批准号:
2883985 - 财政年份:2023
- 资助金额:
$ 46.74万 - 项目类别:
Studentship