Toward a Three-Dimensional Brian Organoid Model to Evaluate Neuro Technology Device

项目摘要/摘要 植入式神经技术，如微电极阵列，为改进提供了实质性的希望 许多神经生成性疾病的状况。然而，植入后不久，异物 发生反应，研究人员认为这会降低这些细胞的稳定性和寿命 设备。已建立的生物标记物，如胶质酸、纤维蛋白和星形胶质细胞增生症等 然而，由于研究了机械失配以评估对设备的响应状态 大脑中的免疫反应还不是很清楚。星形胶质细胞在 大脑的免疫系统，最近，转录组分析证实了钙通道的活性 反应性星形胶质细胞可能是许多神经退行性疾病的潜在生物标记物 论文项目，我将研究星形胶质细胞的反应性与设备之间的相关性 功能。初步研究表明，钙通道异构体对不同的 刺激以诱导反应状态。我将使用组织共培养方法诱导三种类型的 炎性星形细胞模型，并表征每个模型对探测材料硬度的反应性， 装置的特点和细胞结构、代谢和病理生理遗传学的变化 表情。我的目标是阐明神经异物反应中的这一途径，这将给 研究人员成为种植体治疗研究的潜在生物标记物 神经科技。我概述了到目前为止所做的工作、当前和未来的培训战略 学术、专业和教育进步，因为它与本项目相关并与 这是我的职业目标。

Project Summary/Abstract Implantable neurotechnology such as microelectrode arrays offers substantial promise to improve the condition of many neurogenerative diseases. However, shortly after implantation, foreign body response occurs, which is what researchers believe decreases stability and longevity of these devices. Established biomarkers such as glial acid fibrillary protein and astrogliosis and stimuli such as mechanical mismatch are studied to assess the state of response to the devices, however immune response in the brain is not well understood. Astrocytes play an important role in the brain’s immune system and recently, transcriptome analysis has confirmed calcium channel activity of reactive astrocytes to be a potential biomarker in many neurodegenerative diseases, in this dissertation project, I will investigate the correlation between astroglial reactivity and device features. Preliminary studies have demonstrated that calcium channel isoforms respond to different stimuli to induce the reactive state. I will use tissue co-culture methods to induce three types of inflammatory astrocytic models, and characterize each model’s reactivity to probe material stiffness, device features and change in cytoarchitecture, metabolism and pathophysiological genetic expression. I aim to elucidate that pathway in neuro foreign body response, which will give researchers a potential biomarker to target for therapeutic research concerning implantable neurotechnology. I have outlined the work done thus far, current and future training strategies for academic, professional, and educational advancement, as it relates to this project and aligns with this my career goals.