Implementation and Evaluation of Two SARS-CoV-2 Assays for Companion Animal Testing to Expand Vet-LIRN COVID-19 Capacity

项目摘要 严重急性呼吸综合征冠状病毒2(SARS-CoV-2)是一种人畜共患疾病，在 除了在人类大流行中的重要性外，它还造成了溢出感染并继续 在水貂、白尾鹿和仓鼠中传播。具有不同特征的新病毒变异体 不断涌现，具有不同的特征，在某些情况下，这些变体能够 感染原本不容易感染的动物物种。因此，正在进行的监控 通过Vet-LIRN对于早期识别动物体内的传播是必不可少的。考虑到那个同伴 如果SARS-CoV-2能够传播，动物生活在8500万个美国家庭中 在宠物之间或宠物和人类之间，可能会产生巨大的公共健康后果。 以及对野生动物种群的影响。该提案的目标是评估和验证 包括验证环介导的等温扩增(LAMP)在内的新测试方法 在狗和猫的各种样本矩阵中，并执行严格的 市面上有售的酶联免疫吸附试验试剂盒。我们的具体目标包括：目标1：评估 商用比色灯法定性检测4例SARS-CoV-2RNA 从狗和猫身上收集的矩阵。目标2：验证一种商业化的用于 狗和猫在培养传播SARS-CoV-2的能力时产生SARS-CoV-2抗体 血清学检测。这些目标将提供技术人员培训、设备和专业知识，以 为狗和猫的SARS-CoV-2检测提供能力。

Project Summary Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a zoonotic disease that, in addition to its importance in the human pandemic, has caused spillover infection and onward spread in mink, white-tailed deer, and hamsters. New viral variants with different characteristics are constantly emerging, with different characteristics and in some cases, these variants are able to infect species of animals that were not originally susceptible. Therefore, ongoing surveillance through Vet-LIRN is essential for early recognition of spread within animals. Given that companion animals reside in 85 million US households, if SARS-CoV-2 were to gain the ability to spread amongst pets or between pets and humans, there could be immense public health consequence and consequence to wildlife populations. The objective of this proposal is to evaluate and validate novel testing methodologies including validating loop-mediated isothermal amplification (LAMP) in a variety of sample matrices in dogs and cats, and to perform rigorous evaluation of a commercially available ELISA kit. Our specific aims include: Aim 1: Assess the validity of a commercial colorimetric LAMP assay for the qualitative detection of SARS-CoV-2 RNA in 4 novel matrices collected from both dogs and cats. Aim 2: Validate a commercially available ELISA for SARS-CoV-2 antibodies in dogs and cats while building capacity to conduct SARS-CoV-2 serological testing. These aims will provide technician training, equipment, and expertise to provide capacity for SARS-CoV-2 testing in dogs and cats.