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Automated Nucleic Acid Extraction and Purification System to Build Vet-LIRN Testing Capacity

自动化核酸提取和纯化系统，构建 Vet-LIRN 检测能力

基本信息

批准号：
10820696
负责人：
Stephen Douglas Cole
金额：
$ 2.43万
依托单位：
UNIVERSITY OF PENNSYLVANIA
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-09-01 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10820696
关键词：
Automated Nucleic Acid Extraction Purification

项目摘要

There is a public health need for veterinary laboratories to have significant capacity to test animal diagnostic specimens and food/feed samples rapidly and accurately for the presence of important pathogens such as Salmonella, Campylobacter, Listeria and SARS-CoV-2. The FDA Veterinary Laboratory Investigation and Response Network (Vet-LIRN) is a group of veterinary laboratories tasked with protecting the health of humans by being able to rapidly and robustly respond to outbreaks associated with animals and animal products. This equipment-only grant requests support for the purchase of a KingFisher Duo Prime semi-automated nucleic acid extraction system. This equipment will expand the capacity of a Vet-LIRN member laboratory (Ryan Small Animal Hospiital Clinical Microbiology Laboratory at the University of Pennsylvania’s) to perform additional molecular assays for case investigations and enhance their capability to handle additional specimens when surge capacity is potentially needed. It will also the laboratory to more fully engage with proficiency testing and interlaboratory comparison exercises developed by Vet-LIRN. Assays to be developed or implemented using thie equipment by the laboratory will include detection of Salmonella, Campylobacter, Listeria and imipenemase genes from carbapenem-resistant Enterobacterales.

公共卫生需要兽医实验室具备相当大的检测能力快速准确地检测动物诊断标本和食品/饲料样品中是否存在重要的病原体如沙门氏菌、弯曲杆菌、李斯特菌和SARS-CoV-2。FDA的兽医实验室调查和反应网络（Vet-LIRN）是一个兽医组织，负责保护人类健康的实验室，能够快速、有力地应对与动物和动物产品有关的疫情。这种只提供设备的补助金请求支持购买KingFisher Duo Prime半自动核酸萃取系统该设备将扩大Vet-LIRN成员实验室的能力 (Ryan小动物医院临床微生物学实验室宾夕法尼亚州的）进行额外的分子检测的情况下，调查和提高当可能需要浪涌能力时，其处理额外样品的能力。它将此外，实验室更充分地参与能力验证和实验室间 Vet-LIRN开发的比较练习。待开发或实施的试验使用实验室的设备将包括沙门氏菌、弯曲杆菌、李斯特菌的检测和来自碳青霉烯类耐药肠球菌目的亚胺培南酶基因。