Automated Nucleic Acid Extraction and Purification System to Build Vet-LIRN Testing Capacity

自动化核酸提取和纯化系统，构建 Vet-LIRN 检测能力

• 批准号: 10820696
• 负责人: Stephen Douglas Cole
• 金额: $ 2.43万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10820696
• 关键词: Automated; Nucleic; Acid; Extraction; Purification

There is a public health need for veterinary laboratories to have significant capacity to test animal diagnostic specimens and food/feed samples rapidly and accurately for the presence of important pathogens such as Salmonella, Campylobacter, Listeria and SARS-CoV-2. The FDA Veterinary Laboratory Investigation and Response Network (Vet-LIRN) is a group of veterinary laboratories tasked with protecting the health of humans by being able to rapidly and robustly respond to outbreaks associated with animals and animal products. This equipment-only grant requests support for the purchase of a KingFisher Duo Prime semi-automated nucleic acid extraction system. This equipment will expand the capacity of a Vet-LIRN member laboratory (Ryan Small Animal Hospiital Clinical Microbiology Laboratory at the University of Pennsylvania’s) to perform additional molecular assays for case investigations and enhance their capability to handle additional specimens when surge capacity is potentially needed. It will also the laboratory to more fully engage with proficiency testing and interlaboratory comparison exercises developed by Vet-LIRN. Assays to be developed or implemented using thie equipment by the laboratory will include detection of Salmonella, Campylobacter, Listeria and imipenemase genes from carbapenem-resistant Enterobacterales.

出于公共卫生的考虑，兽医实验室需要具备强大的检测能力