Core 2: Heterogeneity of Aging

核心2：老龄化的异质性

• 批准号: 10665585
• 负责人: Christian Michael Metallo
• 金额: $ 20.93万
• 依托单位: SALK INSTITUTE FOR BIOLOGICAL STUDIES
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-30 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10665585
• 关键词: Address; Age; Aging; Algorithms; Artificial Intelligence; Biology of Aging; Cell Aging; Cell model; Cell physiology; Cells; Collaborations; Disease; Ensure; Epigenetic Process; Experimental Designs; Functional disorder; Generations; Genome; Goals; Heterogeneity; Human; Impairment; Individual; Investigation; Knowledge; Leadership; Machine Learning; Mass Spectrum Analysis; Measures; Methods; Modeling; Molecular; Mosaicism; Organ; Organelles; Organoids; Pathology; Process; Proteomics; Records; Research; Research Personnel; Research Project Grants; Research Support; Resolution; Resources; Role; Running; Services; Shock; Site; System; Techniques; Technology; Tissues; Training; Work; age related; aged; cell type; data acquisition; experience; functional decline; high resolution imaging; image processing; imaging modality; imaging system; innovation; instrumentation; metabolomics; multimodality; multiple omics; new technology; next generation sequencing; novel; protein function; sequencing platform; single cell sequencing; temporal measurement; transcriptome sequencing; transcriptomics; virtual

PROJECT SUMMARY – Heterogeneity of Aging Core In line with the pioneering work of Nathan Shock, it is clear that aged tissues accumulate cellular heterogeneity or mosaicism. This heterogeneity is likely a cause of aging due to impairments in both intercellular interactions and the coordination of tissue function. The Heterogeneity of Aging Core (Heterogeneity Core) within the San Diego Nathan Shock Center of Excellence in Basic Biology of Aging (SD-NSC) will enable investigators to probe the heterogeneity of aging over a broad range of scales (from molecules to organelles to single cells and tissues) by providing access to a diverse suite of state-of-the-art instrumentation and analytical technologies, as well as experienced Core staff. The Core will provide specific resources for studying key processes implicated in aging and disease at high resolution, including single-cell next-generation sequencing platforms, high-resolution imaging systems, and mass spectrometry approaches to measure proteomic and metabolomics signatures of aging in cells and tissues, with an emphasis on cell-cell heterogeneity and heterogeneity across tissues. These technologies are rapidly evolving and will continue to do so over the coming years. Utilizing established Core resources with proven track records for staying current with evolving technologies is the most effective way to ensure new innovations in analytical technologies are available to the greatest breadth of aging researchers. The Heterogeneity Core provides specific support for researchers in the aging field by: 1) providing access to specific scientific services, advice, and expertise, 2) developing and disseminating novel methods for correlative data acquisitions, and 3) running on-site and virtual training sessions. The Heterogeneity Core is a critical component in the pipeline of research resources that our SD-NSC will create. The value of this Core is bolstered by the generation of age-equivalent induced cell types and organoids by the Human Cell Models of Aging Core, and novel machine-learning capabilities in the Integrative Models of Aging Core. Together we will provide researchers in the basic biology of aging field with the resources necessary to make key discoveries into the mechanisms by which we age. The Heterogeneity Core will enable studies into the cell-cell and tissue heterogeneity of aging and, ultimately, the contributions and mechanisms by which heterogeneity causes the degeneration and dysfunction that characterizes aging.

项目总结-老化堆芯的异质性 与Nathan Shock的开创性工作一致，很明显，衰老组织积累了细胞异质性 或马赛克。这种异质性很可能是由于细胞间相互作用受损而导致衰老的原因 以及组织功能的协调。老年核心的异质性（异质性核心）在圣 Diego Nathan Shock衰老基础生物学卓越中心（SD-NSC）将使研究人员能够 在广泛的尺度上探索衰老的异质性（从分子到细胞器到单细胞， 组织）通过提供对各种最先进的仪器和分析技术的使用， 以及经验丰富的核心员工。核心将为研究关键过程提供具体资源 涉及衰老和疾病的高分辨率，包括单细胞下一代测序平台， 高分辨率成像系统和质谱方法来测量蛋白质组学和代谢组学 细胞和组织中衰老的特征，强调细胞间异质性和跨 组织中这些技术正在迅速发展，并将在未来几年继续发展。利用 在与不断发展的技术保持同步方面， 有效的方法，以确保分析技术的新创新可供最广泛的 老年研究者Heterocyclic Core通过以下方式为老龄化领域的研究人员提供具体支持： 提供获得具体科学服务、咨询和专门知识的机会，2）开发和传播新的 相关数据采集的方法，以及3）运行现场和虚拟培训课程。的 Heterocycle Core是我们SD-NSC将创建的研究资源管道中的关键组成部分。 该核心的价值得到了年龄等效诱导细胞类型和类器官的生成的支持， 衰老核心的人类细胞模型，以及衰老综合模型中的新型机器学习能力 核心我们将共同为衰老基础生物学领域的研究人员提供必要的资源， 对我们衰老的机制有重大发现。Heterocyclic Core将使以下研究成为可能 衰老的细胞-细胞和组织异质性，以及最终的贡献和机制， 异质性导致老化的退化和功能障碍。