Center on Probes for Molecular Mechanotechnology

分子机械技术探针中心

基本信息

  • 批准号:
    10629919
  • 负责人:
  • 金额:
    $ 146.8万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2023
  • 资助国家:
    美国
  • 起止时间:
    2023-08-10 至 2028-07-31
  • 项目状态:
    未结题

项目摘要

Project summary The proposed Center on Probes for Molecular Mechanotechnology (CPMM) will work to develop and optimize technologies to enable the study of mechanobiology and mechanotransduction pathways in living cells. The CPMM includes three highly synergistic Technology Development Projects (TDPs) that will be led by Alexa Mattheyses, Khalid Salaita, and Yonggang Ke who have a strong track record of jointly publishing and working together to developed tension probe technologies. In TDP#1: High resolution probes for mechanobiology, we will create “indestructible” probes that can push the limits of spatial and orientation resolution for the DNA tension probe technology. Tension-PAINT imaging will be refined to achieve realtime 20 nm spatial mapping of forces and to combine this with immunostaining to map the proteins that assemble within proximity to mechanically active receptors. Force orientation will be mapped using fluorescence polarization methods with turn-key commercial microscopes. In TDP#2: Probes for mechanical tagging, we will develop methods of force-induced tagging. The central design feature is a DNA probe that mediates a binding event or dissociation event at threshold levels of force. Cells are tagged based on the magnitude and frequency of mechanical events generated by a cell surface receptors. This TDP will lead to high-throughput analysis of cells using flow cytometry and will also allow for proteomic analysis to open the door to “mechanomics”. Under TDP#3: Amplified force sensors, the central technology here is responsive DNA structures that amplify mechanical inputs. The CPMM has nine associated inaugural Driving Biomedical Projects (DBPs) led by a team of geographically diverse collaborators. DBPs #1-#4 are focused on mechanobiology of T cells and use CPMM tools to test the mechanosensor function of the T cell receptor (TCR) and the adhesion receptor LFA-1. DBP#5 focuses on the heterogeneity in cancer cells. DBP#6 and #7 target the mechanosensor responses of platelets. Finally, DBP#8 and #9 address fundamental questions of the role of mechanics in focal adhesions. Our prototype TDP technologies provide methods to measure molecular forces with the same ease and simplicity as that of immunostaining, flow cytometry, PCR and ELISA. But unlike these mainstream techniques, mechano-imaging, mechano-PCR, mechano-flow, and mechano-ELISA are not commercialized. Hence, the reagents and surface preparation protocols and data analysis routines have to be custom prepared by the end user. This can be challenging to the non-expert and is not routine. Therefore, the CPMM will integrate a strong Community Engagement (CE) component. CE activities will focus on hands-on training workshops, publication of methods articles, virtual seminar series, industry engagement, a strong web presence and engagement with three key mechanobiology conferences that will help accelerate adoption of the tension probe technology. These CE activities will ultimately lead to commercialization which will enable wide spread dissemination across the various cell biology communities.
项目总结

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Khalid S Salaita其他文献

Khalid S Salaita的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Khalid S Salaita', 18)}}的其他基金

Mechano-ID for tagging immune cells
用于标记免疫细胞的 Mechano-ID
  • 批准号:
    10608815
  • 财政年份:
    2022
  • 资助金额:
    $ 146.8万
  • 项目类别:
Rolosense: An innovative platform for automatic mobile phone readout of active SARS-CoV-2 particles (RADx-rad / SEED Administrative Supplement)
Rolosense:用于自动手机读取活性 SARS-CoV-2 颗粒的创新平台(RADx-rad / SEED 行政补充文件)
  • 批准号:
    10648924
  • 财政年份:
    2022
  • 资助金额:
    $ 146.8万
  • 项目类别:
Mechano-ID for tagging immune cells
用于标记免疫细胞的 Mechano-ID
  • 批准号:
    10664365
  • 财政年份:
    2022
  • 资助金额:
    $ 146.8万
  • 项目类别:
Rolosense: An innovative platform for automatic mobile phone readout of active SARS-CoV-2 particles
Rolosense:用于自动手机读取活性 SARS-CoV-2 颗粒的创新平台
  • 批准号:
    10321002
  • 财政年份:
    2020
  • 资助金额:
    $ 146.8万
  • 项目类别:
Rolosense: An innovative platform for automatic mobile phone readout of active SARS-CoV-2 particles
Rolosense:用于自动手机读取活性 SARS-CoV-2 颗粒的创新平台
  • 批准号:
    10264612
  • 财政年份:
    2020
  • 资助金额:
    $ 146.8万
  • 项目类别:
Catalytic Nanotherapies to Treat Lung Disease
治疗肺部疾病的催化纳米疗法
  • 批准号:
    9977246
  • 财政年份:
    2018
  • 资助金额:
    $ 146.8万
  • 项目类别:
Catalytic Nanotherapies to Treat Lung Disease
治疗肺部疾病的催化纳米疗法
  • 批准号:
    10169812
  • 财政年份:
    2018
  • 资助金额:
    $ 146.8万
  • 项目类别:
Catalytic Nanotherapies to Treat Lung Disease
治疗肺部疾病的催化纳米疗法
  • 批准号:
    10227119
  • 财政年份:
    2018
  • 资助金额:
    $ 146.8万
  • 项目类别:
Catalytic Nanotherapies to Treat Lung Disease
治疗肺部疾病的催化纳米疗法
  • 批准号:
    10463234
  • 财政年份:
    2018
  • 资助金额:
    $ 146.8万
  • 项目类别:
Developing a Bioanalytical Toolkit to Study the Mechanobiology of Juxtacrine Signaling
开发生物分析工具包来研究近分泌信号传导的力学生物学
  • 批准号:
    9894683
  • 财政年份:
    2017
  • 资助金额:
    $ 146.8万
  • 项目类别:

相似海外基金

SHINE: Origin and Evolution of Compressible Fluctuations in the Solar Wind and Their Role in Solar Wind Heating and Acceleration
SHINE:太阳风可压缩脉动的起源和演化及其在太阳风加热和加速中的作用
  • 批准号:
    2400967
  • 财政年份:
    2024
  • 资助金额:
    $ 146.8万
  • 项目类别:
    Standard Grant
Collaborative Research: FuSe: R3AP: Retunable, Reconfigurable, Racetrack-Memory Acceleration Platform
合作研究:FuSe:R3AP:可重调、可重新配置、赛道内存加速平台
  • 批准号:
    2328975
  • 财政年份:
    2024
  • 资助金额:
    $ 146.8万
  • 项目类别:
    Continuing Grant
EXCESS: The role of excess topography and peak ground acceleration on earthquake-preconditioning of landslides
过量:过量地形和峰值地面加速度对滑坡地震预处理的作用
  • 批准号:
    NE/Y000080/1
  • 财政年份:
    2024
  • 资助金额:
    $ 146.8万
  • 项目类别:
    Research Grant
Market Entry Acceleration of the Murb Wind Turbine into Remote Telecoms Power
默布风力涡轮机加速进入远程电信电力市场
  • 批准号:
    10112700
  • 财政年份:
    2024
  • 资助金额:
    $ 146.8万
  • 项目类别:
    Collaborative R&D
Collaborative Research: FuSe: R3AP: Retunable, Reconfigurable, Racetrack-Memory Acceleration Platform
合作研究:FuSe:R3AP:可重调、可重新配置、赛道内存加速平台
  • 批准号:
    2328973
  • 财政年份:
    2024
  • 资助金额:
    $ 146.8万
  • 项目类别:
    Continuing Grant
Collaborative Research: FuSe: R3AP: Retunable, Reconfigurable, Racetrack-Memory Acceleration Platform
合作研究:FuSe:R3AP:可重调、可重新配置、赛道内存加速平台
  • 批准号:
    2328972
  • 财政年份:
    2024
  • 资助金额:
    $ 146.8万
  • 项目类别:
    Continuing Grant
Collaborative Research: A new understanding of droplet breakup: hydrodynamic instability under complex acceleration
合作研究:对液滴破碎的新认识:复杂加速下的流体动力学不稳定性
  • 批准号:
    2332916
  • 财政年份:
    2024
  • 资助金额:
    $ 146.8万
  • 项目类别:
    Standard Grant
Collaborative Research: A new understanding of droplet breakup: hydrodynamic instability under complex acceleration
合作研究:对液滴破碎的新认识:复杂加速下的流体动力学不稳定性
  • 批准号:
    2332917
  • 财政年份:
    2024
  • 资助金额:
    $ 146.8万
  • 项目类别:
    Standard Grant
Collaborative Research: FuSe: R3AP: Retunable, Reconfigurable, Racetrack-Memory Acceleration Platform
合作研究:FuSe:R3AP:可重调、可重新配置、赛道内存加速平台
  • 批准号:
    2328974
  • 财政年份:
    2024
  • 资助金额:
    $ 146.8万
  • 项目类别:
    Continuing Grant
Radiation GRMHD with Non-Thermal Particle Acceleration: Next-Generation Models of Black Hole Accretion Flows and Jets
具有非热粒子加速的辐射 GRMHD:黑洞吸积流和喷流的下一代模型
  • 批准号:
    2307983
  • 财政年份:
    2023
  • 资助金额:
    $ 146.8万
  • 项目类别:
    Standard Grant
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了