Rolosense: An innovative platform for automatic mobile phone readout of active SARS-CoV-2 particles (RADx-rad / SEED Administrative Supplement)

Rolosense：用于自动手机读取活性 SARS-CoV-2 颗粒的创新平台（RADx-rad / SEED 行政补充文件）

• 批准号: 10648924
• 负责人: Khalid S Salaita
• 金额: $ 50万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-17 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10648924
• 关键词: 2019-nCoV; Administrative Supplement; Biological; Biological Assay; Car Phone; Enzymes; Funding; Goals; Ligand Binding; Pathway interactions; RADx Radical; RNA; Reproducibility; Resources; Risk; Sampling; Speed; Technology; Viral; Virus; commercialization; cost; innovation; particle; response

Project summary: The supplement application is in response to the RADx-rad announcement on the opportunity of additional resources and funding support to facilitate the pathway to commercialization. As part of this supplement we will pursue specific milestones to de-risk the Rolosense technology and further move it toward commercialization. A major goal will be to introduce multiplexing capabilities to simultaneously detect multiple viral targets from the same sample. Encoded Rolosense particles with unique virus-binding ligands will be employed to achieve this goal. Multiplexing to detect multiple viral targets will help distinguish the capabilities of Rolosense from that of the current state-of-the-art. Another goal is to increase the speed and robustness of the assay to facilitate direct sensing from breath condensate samples. This second goal will be pursued by further developing fuel-free Rolosense that eliminates the need for RNA and RNaseH enzyme in the assay. We see this as a critical step to reduce the cost of the assay and to increase rigor and reproducibility to achieve the commercialization goals without concern for RNaseA found in most biological samples.

项目概要： 补充申请是为了响应RADx-rad宣布的机会， 增加资源和资金支持，以促进商业化。一部分 我们将追求具体的里程碑，以降低Rolosense技术的风险， 进一步走向商业化。一个主要目标将是引入多路复用 能够同时检测来自同一样品的多个病毒靶标。编码 将采用具有独特病毒结合配体的Rolosense颗粒来实现这一目标。 多重检测多个病毒靶点将有助于区分Rolosense与 另一个目标是提高系统的速度和鲁棒性。 分析以促进从呼吸冷凝物样品的直接感测。第二个目标将是 通过进一步开发无需燃料的Rolosense，消除了对RNA和RNaseH的需求， 酶在检测中我们认为这是降低测定成本并增加测定效率的关键步骤。 在不考虑RNaseA的情况下实现商业化目标的严谨性和重现性 在大多数生物样本中。

项目成果

Rolosense: Mechanical detection of SARS-CoV-2 using a DNA-based motor.

Rolosense：使用基于 DNA 的电机对 SARS-CoV-2 进行机械检测。

• DOI: 10.1101/2023.02.27.530294
• 发表时间: 2023
• 期刊: bioRxiv : the preprint server for biology
• 作者: Piranej,Selma;Zhang,Luona;Bazrafshan,Alisina;Marin,Mariana;Melikyan,GregoryB;Salaita,Khalid
• 通讯作者: Salaita,Khalid

High-throughput microbead assay system with a portable, cost-effective Wi-Fi imaging module, and disposable multi-layered microfluidic cartridges for virus and microparticle detection, and tracking.

• DOI: 10.1007/s10544-023-00661-3
• 发表时间: 2023-06-07
• 期刊: BIOMEDICAL MICRODEVICES
• 影响因子: 2.8
• 作者: Castro, Jorge Manrique;Sommerhage, Frank;Khanna, Rishika;Childs, Andre;DeRoo, David;Rajaraman, Swaminathan
• 通讯作者: Rajaraman, Swaminathan