Molecular Mechanisms

项目摘要/抽象分子机制核心 宾宁顿/路易斯安那州NORC以“营养、肥胖和新陈代谢”为主题促进研究 生命中的健康“。在这一主题下，NORC分子机制核心寻求促进 通过为NORC成员提供获得现代生物医学研究技术的研究进展 通常超过单个实验室的能力，特别是那些刚开始调查的实验室 事业。了解构成肥胖和代谢的致病框架的分子病因学 疾病，捕捉基因表达的技术--生理和表型的驱动力--是 进行机械研究的主要必要性。 因此，分子机制核心提供了对基因评估技术的交钥匙访问 向NORC调查人员表达。这些范围从复杂RNA中的系统生物学水平的表达图谱 混合物、单细胞RNA测序以揭示基于基因的细胞组成和组织多样性 表达，到高倍显微镜和三维成像方法来定位基因产物，以及 确定表情随外部因素发生变化的位置。总之，这些方法提供了 一个关于细胞和组织功能如何受营养、肥胖或 代谢性疾病。 核心提供围绕一般实验工作流构建的服务，这些服务组织在三个特定的 目标：提供与分子相关的实验设计和统计方面的咨询服务 生物成像和功能基因组学技术(目标1)；专门知识和直接技术支持 实验(目标2)；分子生物成像和生物信息学的数据处理服务和生物信息学支持 功能基因组学实验(目标3)。所有三个目标中的服务都基于专业知识、技能和 在核心领导和员工方面有经验，严格的质量控制措施和仪器维护， 与核心用户的交互完全透明，并且可以灵活地定制协议以满足 NORC成员的个别项目。随着NORC成员需求的变化和技术的进步， CORE寻求将新的方法引入在线，以满足当前和未来NORC调查人员的需求。

PROJECT SUMMARY/ABSTRACT MOLECULAR MECHANISMS CORE The Pennington/Louisiana NORC promotes research under the theme of “Nutrition, Obesity, and Metabolic Health through the Lifespan”. Within that theme, the NORC Molecular Mechanisms Core seeks to facilitate research progress by providing NORC Members with access to modern biomedical research technology that typically exceed the capabilities of individual laboratories, particularly those of investigators at the start of their careers. To understand the molecular etiology that forms the pathogenic framework for obesity and metabolic disease, technologies that capture gene expression -- the driving force of physiology and phenotype -- are of primary necessity for conducting mechanistic research. Therefore, the Molecular Mechanisms Core provides turn-key access to technologies assessing gene expression to NORC investigators. These range from systems-biology level expression profiling in complex RNA mixtures, single cell RNA sequencing to reveal cellular composition and tissue diversity based on gene expression, to high power microscopy and three-dimensional imaging methods to locate gene products, and determine where expression changes occur in response to external factors. Together, these methods provide an integrated mechanistic perspective on how cell and tissue functions are affected by nutrition, obesity, or metabolic disease. The Core offers services built around the general experimental workflow that are organized in three Specific Aims: consulting services on the experimental design and statistical considerations pertaining to molecular bioimaging and functional genomics technology (Aim 1); expertise and direct technological support for experiments (Aim 2); and data processing services and bioinformatics support for molecular bioimaging and functional genomics experiments (Aim 3). Services in all three Aims are based on the expertise, skills, and experience present in the Core leadership and staff, strict quality control measures and instrument maintenance, full transparency in interaction with Core users, and the flexibility to customize protocols to suit the needs of individual NORC-member projects. As the needs of NORC members change and as technology moves ahead, the Core seeks to bring new methods online to serve the needs of current and future NORC investigators.