Molecular Mechanisms

项目摘要： 揭示疾病代谢基础的现代生物医学研究越来越依赖于 复杂技术的应用。在许多情况下，与采购和维护有关的费用 除了运行这些技术所需的技术专长外， 各实验室的能力-特别是那些刚开始职业生涯的研究人员的能力。因此，我们认为， COBRE分子机制核心的总体目标是为研究人员提供这样的 功能基因组学和分子生物成像领域的技术，从而帮助他们应用 在他们的研究项目中使用艺术方法。该核心力求通过两个具体目标实现这一目标： 具体目标1：为COBRE研究人员提供分子生物成像方面的指导支持， 功能基因组学技术现代生物医学技术的应用本身并不导致 有意义或有影响力的科学进步;它需要对特定技术的力量有清晰的理解 让科学从数据中诞生因此，该核心将作为知识和咨询资源， COBRE调查员，当涉及到生物成像和功能基因组学实验，并提供指导 使技术方法与每个项目的科学目标相匹配。因此，本目标的目标 是确保COBRE的调查人员，而不是把技术看作是一个可能无法克服的障碍， 事实上，他们可以满怀信心地接受这些新技术，以促进他们的科学。 具体目标2：为COBRE研究人员的实验提供直接的技术支持， 使用分子生物成像和功能基因组学方法。为了了解形成的分子病因学 疾病的致病框架，捕获基因表达变化的技术是主要的 进行机理研究的必要性，核心的关键技术是三维的 在整体组织中的基因表达成像，以及使用单细胞RNA评估基因表达 测序核心将为COBRE调查人员提供这些技术的交钥匙访问，以便 确保他们不会不必要地陷入实际操作的技术细节，而是能够完全集中精力 他们的研究项目的科学性，以及他们的整体生产力。 此外，核心，依靠其国家卫生研究院的建议审查专业知识，并确保实验符合 NIH的严谨性和重现性标准，从而有助于一个强有力的前提下，可以提供指导 COBRE研究人员需要一个有竞争力的环境来产生NIH拨款提案。

Project Summary: Modern biomedical research to uncover the metabolic basis of disease is increasingly dependent on the application of complex technology. In many cases, the costs associated with the acquisition and maintenance of necessary equipment, in addition to the technical expertise needed to run these technologies, typically exceed the capabilities of individual laboratories - particularly those of investigators at the start of their careers. Hence, the overarching goal for the COBRE Molecular Mechanisms Core is to provide investigators with such technologies in the realm of functional genomics and molecular bioimaging, and thus help them to apply state of the art approaches in their research projects. The Core seeks to achieve this goal in two Specific Aims: SPECIFIC AIM 1: To provide COBRE investigators with mentoring support concerning molecular bioimaging and functional genomics technology. Application of modern biomedical technology per se does not lead to meaningful or impactful scientific progress; it requires a clear understanding of the power of specific technologies for science to emerge from data. Therefore, the Core will serve as an intellectual and advisory resource for COBRE investigators when it comes to bioimaging and functional genomics experiments, and provide mentoring so that technological approaches match up with the scientific goal of each project. Hence, the goal for this Aim is to ensure that COBRE investigators, rather than viewing technology as a perhaps unsurmountable obstacle, can in fact embrace these new technologies with confidence to further their science. SPECIFIC AIM 2: To provide COBRE investigators with direct technological support for their experiments that use molecular bioimaging and functional genomics approaches. To understand the molecular etiology that forms the pathogenic framework for disease, technologies that capture gene expression changes are of primary necessity for conducting mechanistic research, and the key technologies for the Core are three-dimensional imaging of gene expression in whole-mount tissues, and assessment of gene expression using single-cell RNA sequencing. The Core will provide COBRE investigators with turn-key access to these technologies in order to ensure that they do not get needlessly bogged down in the hands-on technical minutiae, but can fully concentrate on the science of their research projects, and on their overall productivity. Furthermore, the Core, by relying on its NIH proposal review expertise, and by ensuring that experiments meet NIH rigor and reproducibility standards and thus contribute to a strong premise, can provide the mentoring environment needed for COBRE investigators to generate competitive NIH grant proposals.