COBRE: UNE MED CTR: CORE B: MOUSE GENOME ENGINEERING

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。这个Cobre提议的目标之一是建立一个三角的UNMC-Creighton-Boys town NRH作为公认的神经感觉发展卓越研究中心。随着这三个机构对人类健康的承诺，小鼠将成为未来这一主题生物医学研究的最重要的模式生物。因此，鼠标核心的存在和供应不仅将通过现场建立相关的鼠标模型使最初的Cobre调查人员受益，而且一旦最初的Cobre调查人员轮换离开，新的调查人员将被带上船，它将作为一个必要的招聘工具。我们预计，这些新兵也将专注于小鼠，使用基因操纵的小鼠来推进他们的研究。特别是，在Mouse方面经验有限的初级调查人员将从Mouse核心支持服务中受益匪浅。此外，我们期望这一建议的几个项目的表达谱实验将产生值得为各自的基因产生新的转基因和敲除小鼠模型的结果，以便研究它们在神经感觉发育中的功能。因此，我们相信，鼠标核心的提供将大大提高科布雷提案所促进的新的外部资金申请的竞争力。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. One goal of this COBRE proposal is to establish the triangle UNMC-Creighton-Boys Town NRH as a recognized Center of Research Excellence in Neurosensory Development. With the commitment of all three institutions to human health care, the mouse will be the paramount model organism for future biomedical research on this topic. The presence and availability of a Mouse Core will, therefore, not only benefit the initial COBRE Investigators through the on-site establishment of the pertinent mouse models, but will serve as an essential recruiting tool once the initial COBRE Investigators will rotate off and new Investigators will be brought aboard. We anticipate that such recruits will also focus on the mouse, use genetically manipulated mice to advance their research. In particular, junior Investigators with limited experience with mice would greatly benefit from Mouse Core support services. Furthermore, we expect that the expression profiling experiments of several projects of this proposal will yield results that merit the generation of new transgenic and knockout mouse models for the respective genes in order to study their function in neurosensory development. We are therefore convinced that the availability of a Mouse Core will significantly enhance the competitiveness of new applications for extramural funding that are fostered by this COBRE proposal.