Asthma and EC coupling in airway smooth muscle cells

气道平滑肌细胞中的哮喘和 EC 耦合

• 批准号: 6784096
• 负责人: SEAN M WILSON
• 金额: $ 7.25万
• 依托单位: UNIVERSITY OF MISSISSIPPI
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6784096
• 关键词: allergens; asthma; biological signal transduction; calcium channel; calcium flux; confocal scanning microscopy; immunocytochemistry; laboratory rat; muscle contraction; polymerase chain reaction; receptor coupling; receptor sensitivity; respiratory airway pressure; respiratory hypersensitivity; respiratory muscles; smooth muscle

DESCRIPTION (provided by applicant): Allergic asthma is the result of airway sensitization and subsequent challenge with allergen, which induces a reduction in gas exchange at the alveoli. Contributing to this impaired gas exchange there is a marked proliferation of airway smooth muscle cells (ASMCs) that also become hypersensitive to both specific (allergen) and nonspecific (acetylcholine) challenge. Given that increases in the intracellular Ca2+ concentration ([Ca2+]i) are integral to airway smooth muscle (ASM) contraction, the central hypothesis is that alterations in Ca2+ signaling are a component of asthma-induced hypersensitivity of ASM. A number of reports indicate that inflammatory agents cause [Ca2+]I to increase in ASMCs. However, the interaction between inflammatory mediators and/or allergic cytokines and increases in ASM cytosolic Ca2+ is unresolved. The two specific aims outlined in the proposal are designed to examine the interaction between inflammatory stimulation, asthma, and cytosolic Ca2+. Specific Aim 1 tests the hypothesis that allergen sensitization and challenge induced ASMC hypersensitivity is due to enhanced intracellular Ca2+ release and extracellular Ca2+ entry. [Ca2+]i will be measured in isolated ASMCs from control and ovalbumin allergen sensitized and challenged Brown-Norway rats, which mimics allergen induced asthma in humans using global Ca2+ imaging and real-time laser scanning confocal microscopy techniques. Changes in basal [Ca2+]i, Kd of Ca2+ increases, change in the spatial and temporal aspects of Ca2+ signaling, or changes in the rate and routes of Ca2+ entry and cytosolic Ca2+ removal with serotonin exposure will be determined. Specific Aim 2 tests the hypothesis that allergen sensitization and challenge induced ASM hypersensitivity is the result of a change in expression of gene products that directly alter Ca2+ signaling. Quantitative RT-PCR and immunohistochemistry techniques will be used to determine if allergen sensitization and challenge changes the expression of non-selective cation channels as well as changes the expression and spatial location of ryanodine and IP3 receptors. Experimental results from these two aims will provide critical pilot data towards the long-term objectives that are to understand the role of changes in Ca2+ signaling to asthma induced hypersensitivity and to elucidate the cell signaling pathways that lead to airway hyperresponsiveness.

描述（由申请方提供）：过敏性哮喘是气道致敏和随后用过敏原激发的结果，其诱导肺泡气体交换减少。 气道平滑肌细胞（ASMC）的显著增殖也导致这种受损的气体交换，所述ASMC也变得对特异性（变应原）和非特异性（乙酰胆碱）挑战过敏。 鉴于细胞内Ca 2+浓度（[Ca 2 +]i）的增加是气道平滑肌（ASM）收缩不可或缺的，中心假设是Ca 2+信号的改变是哮喘诱导的ASM超敏反应的一个组成部分。 许多报告表明，炎症因子导致ASMCs [Ca 2 +]I增加。 然而，炎症介质和/或过敏性细胞因子之间的相互作用和ASM胞质Ca 2+的增加是未解决的。 该提案中概述的两个具体目标旨在研究炎症刺激，哮喘和细胞溶质Ca 2+之间的相互作用。 特异性目的1检验了过敏原致敏和激发诱导ASMC超敏反应是由于细胞内Ca 2+释放和细胞外Ca 2+进入增强的假设。 [Ca2+]i将在来自对照和卵清蛋白过敏原致敏和激发的Brown-Norway大鼠的分离的ASMC中测量，所述Brown-Norway大鼠使用全局Ca 2+成像和实时激光扫描共聚焦显微镜技术模拟过敏原诱导的人类哮喘。 将确定基础[Ca 2 +]i、Ca 2+增加的Kd的变化、Ca 2+信号传导的空间和时间方面的变化或随着5-羟色胺暴露的Ca 2+进入和胞质Ca 2+去除的速率和途径的变化。 特异性目的2检验了过敏原致敏和激发诱导的ASM超敏反应是直接改变Ca 2+信号传导的基因产物表达变化的结果这一假设。 定量RT-PCR和免疫组织化学技术将用于确定过敏原致敏和激发是否改变非选择性阳离子通道的表达以及兰尼碱和IP 3受体的表达和空间位置。 这两个目标的实验结果将为长期目标提供关键的试点数据，即了解Ca 2+信号变化对哮喘诱导的超敏反应的作用，并阐明导致气道高反应性的细胞信号通路。

项目成果

Enhanced capacitative calcium entry and sarcoplasmic-reticulum calcium storage capacity with advanced age in murine mesenteric arterial smooth muscle cells.

随着年龄的增长，小鼠肠系膜动脉平滑肌细胞的钙进入能力和肌浆网钙储存能力增强。

• DOI: 10.1016/j.exger.2008.10.007
• 发表时间: 2009-03
• 期刊: EXPERIMENTAL GERONTOLOGY
• 影响因子: 3.9
• 作者: Goyal, Ravi;Angermann, Jeff E.;Ostrovskaya, Olga;Buchholz, John N.;Smith, Gregory D.;Wilson, Sean M.
• 通讯作者: Wilson, Sean M.