MISSISSIPPI COBRE: CORE C: IN VITRO PHARMACOLOGY CORE

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。目的：对天然产物进行科学探索以开发精神药物治疗是一个尚未充分研究的研究领域。体外药理学核心将提供一些与理解精神活性天然产物的作用机制相关的功能，这些机制可以导致新疗法的发展。具体来说，Core C将提供内部筛选化合物生物活性的能力，并确定作用位点和作用机制。这些核心功能依赖于使用最先进的技术以及经过时间考验的实验室方法。使用多标签阅读器技术，该核心将能够快速评估保存在密西西比大学储存库中的化合物和提取物的特定受体活性。对铅化合物作用的机制研究将利用细胞电生理学和成像技术进行评估。受体亲和力测定和动力学分析将完善核心C的技术曲目。表1给出了常规检测的概述。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objectives: The scientific exploration of natural products for the development of psychotropic therapies is an understudied area of research. The In Vitro Pharmacology Core will provide several functions related to understanding the mechanisms of actions of psychoactive natural products that can lead to the development of new therapies. Specifically, Core C will provide the ability in house to screen compounds for biological activity and determine the site(s) as well as mechanism(s) of action. These core functions are dependent on the use of state of the art technologies as well as time-tested laboratory methodologies. Using multi-label reader technologies, the core will be able to rapidly assess compounds and extracts held in the repositories at the University of Mississippi for specific receptor activities. Mechanistic studies into the actions of lead compounds will be assessed using cellular electrophysiology and imaging techniques. Receptor affinity determinations and kinetic analyses will round out Core C's repertoire of techniques. Table 1 presents an overview of the assays that are to be run routinely.